MARCHF11 (NM_001102562) Human Untagged Clone

CAT#: SC316932

41344 (untagged)-Human membrane-associated ring finger (C3HC4) 11 (MARCH11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARCHF11
• Synonyms: MARCH-XI; MARCH11; RNF226
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316932 representing NM_001102562.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGCTTTGAGGGCGGCCACGGCGGCAGTCGGTGTCGCGGGGCGGAGAGCGGGGACGCCGAGCCTCCC
  CCGCAACCTCCCCCGCCGCCGCCGCCGACGCCGCCGCCGGGAGAGCCGGCCCCGGTCCCCGCGGCCCCG
  CGCTACCTGCCGCCGCTGCCCGCGTCCCCCGAGACCCCCGAGCGCGCCGCGGGGCCAAGCGAGCCGCTA
  GGGGAGGTGGCCCCGCGGTGCAGGGGAGCGGACGAGCTGCCGCCTCCGCCCCTGCCCCTGCAGCCCGCC
  GGCCAGGAAGTGGCGGCGGCCGGCGACTCCGGGGAAGGTCCGAGGCGCCTCCCGGAGGCGGCAGCAGCG
  AAAGGCGGCCCCGGGGAGTCTGAGGCCGGCGCGGGCGGCGAGCGCGAGCGGCGGGGCGCCGGAGACCAG
  CCCGAGACGCGCTCGGTGTGCAGCAGCCGCAGCAGCAGCAGTGGCGGCGGCGACCAGCGCGCTGGGCAC
  CAGCACCAGCACCACCAGCCCATCTGCAAGATCTGCTTCCAGGGCGCGGAGCAGGGTGAGTTGTTGAAC
  CCCTGCCGATGTGATGGGTCAGTTCGGTATACACATCAGCTGTGCCTGCTAAAATGGATCAGTGAGAGA
  GGTTCCTGGACCTGTGAACTTTGCTGTTATAGATACCATGTTATAGCCATTAAAATGAAACAACCTTGC
  CAGTGGCAGAGCATTTCTATAACACTGGTTGAGAAAGTTCAGATGATTGCTGTAATCCTAGGATCCCTG
  TTCTTAATAGCCAGTGTGACTTGGCTCCTCTGGTCAGCCTTCAGCCCCTATGCAGTGTGGCAGAGGAAG
  GACATCCTTTTTCAGATCTGCTATGGAATGTATGGTTTTATGGATCTAGTGTGCATAGGTCTCATTGTT
  CATGAAGGAGCTGCAGTTTACAGAGTGTTTAAGCGCTGGCGAGCTGTGAATTTGCACTGGGATGTGTTA
  AATTATGACAAAGCCACAGACATCGAAGAAAGCAGCCGGGGAGAGTCTTCCACAAGTAGGACTTTGTGG
  TTGCCATTGACAGCTCTGCGGAACAGAAACTTGGTCCACCCAACTCAGTTAACCTCACCAAGGTTTCAG
  TGTGGCTATGTGTTATTGCACCTGTTCAATCGGATGAGGCCCCATGAAGACTTATCAGAAGATAACAGC
  TCGGGGGAGGTTGTGATGAGAGTGACTTCAGTGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001102562
• Insert Size: 1209 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102562.1
• RefSeq Size: 1528 bp
• RefSeq ORF: 1209 bp
• Locus ID: 441061
• UniProt ID: A6NNE9
• Cytogenetics: 5p15.1
• MW: 43.9 kDa
• Gene Summary: MARCH11 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). These enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their intracellular transport. March11 appears to have a role in ubiquitin-mediated protein sorting in the trans-Golgi network (TGN)-multivesicular body (MVB) transport pathway (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]