TMEM16A (ANO1) (NM_018043) Human Untagged Clone

CAT#: SC316851

ANO1 (untagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMEM16A
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_018043 edited
  ATGGACTACCACGAGGATGACAAGCGCTTCCGCAGGGAGGAGTACGAGGGCAACCTCCTG
  GAGGCGGGCCTGGAGCTGGAGCGGGACGAGGACACTAAAATCCACGGAGTCGGGTTTGTG
  AAAATCCATGCCCCCTGGAACGTGCTGTGCAGAGAGGCCGAGTTTCTGAAACTGAAGATG
  CCGACGAAGAAGATGTACCACATTAATGAGACCCGTGGCCTCCTGAAAAAAATCAACTCT
  GTGCTCCAGAAAATCACAGATCCCATCCAGCCCAAAGTGGCTGAGCACAGGCCCCAGACC
  ATGAAGAGACTCTCCTATCCCTTCTCCCGGGAGAAGCAGCATCTATTTGACTTGTCTGAT
  AAGGATTCCTTTTTCGACAGCAAAACCCGGAGCACGATTGTCTATGAGATCTTGAAGAGA
  ACGACGTGTACAAAGGCCAAGTACAGCATGGGCATCACGAGCCTGCTGGCCAATGGTGTG
  TACGCGGCTGCATACCCACTGCACGATGGAGACTACAACGGTGAAAACGTCGAGTTCAAC
  GACAGAAAACTCCTGTACGAAGAGTGGGCACGCTATGGAGTTTTCTATAAGTACCAGCCC
  ATCGACCTGGTCAGGAAGTATTTTGGGGAGAAGATCGGCCTGTACTTCGCCTGGCTGGGC
  GTGTACACCCAGATGCTCATCCCTGCCTCCATCGTGGGAATCATTGTCTTCCTGTACGGA
  TGCGCCACCATGGATGAAAACATCCCCAGCATGGAGATGTGTGACCAGAGACACAATATC
  ACCATGTGCCCGCTTTGCGACAAGACCTGCAGCTACTGGAAGATGAGCTCAGCCTGCGCC
  ACGGCCCGCGCCAGCCACCTCTTCGACAACCCCGCCACGGTCTTCTTCTCTGTCTTCATG
  GCCCTCTGGGCTGCCACCTTCATGGAGCACTGGAAGCGGAAACAGATGCGACTCAACTAC
  CGCTGGGACCTCACGGGCTTTGAAGAGGAAGAGGAGGCTGTCAAGGATCATCCTAGAGCT
  GAATACGAAGCCAGAGTCTTGGAGAAGTCTCTGAAGAAAGAGTCCAGAAACAAAGAGACT
  GACAAAGTGAAGCTGACATGGAGAGATCGGTTCCCAGCCTACCTCACTAACTTGGTCTCC
  ATCATCTTCATGATTGCAGTGACGTTTGCCATCGTCCTCGGCGTCATCATCTACAGGATC
  TCCATGGCCGCCGCCTTGGCCATGAACTCCTCCCCCTCCGTGCGGTCCAACATCCGGGTC
  ACAGTCACAGCCACCGCAGTCATCATCAACCTAGTGGTCATCATCCTCCTGGACGAGGTG
  TATGGCTGCATAGCCCGATGGCTCACCAAGATCGAGGTCCCAAAGACGGAGAAAAGCTTT
  GAGGAGAGGCTGATCTTCAAGGCTTTCCTGCTGAAGTTTGTGAATTCCTACACCCCCATC
  TTTTACGTGGCGTTCTTCAAAGGCCGGTTTGTTGGACGCCCGGGCGACTACGTGTACATT
  TTCCGTTCCTTCCGAATGGAAGAGTGTGCGCCAGGGGGCTGCCTGATGGAGCTATGCATC
  CAGCTCAGCATCATCATGCTGGGGAAACAGCTGATCCAGAACAACCTGTTCGAGATCGGC
  ATCCCGAAGATGAAGAAGCTCATCCGCTACCTGAAGCTGAAGCAGCAGAGCCCCCCTGAC
  CACGAGGAGTGTGTGAAGAGGAAACAGCGGTACGAGGTGGATTACAACCTGGAGCCCTTC
  GCGGGCCTCACCCCAGAGTACATGGAAATGATCATCCAGTTTGGCTTCGTCACCCTGTTT
  GTCGCCTCCTTCCCCCTGGCCCCACTGTTTGCGCTGCTGAACAACATCATCGAGATCCGC
  CTGGACGCCAAAAAGTTTGTCACTGAGCTCCGAAGGCCGGTAGCTGTCAGAGCCAAAGAC
  ATCGGAATCTGGTACAATATCCTCAGAGGCATTGGGAAGCTTGCTGTCATCATCAATGCC
  TTCGTGATCTCCTTCACGTCTGACTTCATCCCGCGCCTGGTGTACCTCTACATGTACAGT
  AAGAACGGGACCATGCACGGCTTCGTCAACCACACCCTCTCCTCCTTCAACGTCAGTGAC
  TTCCAGAACGGCACGGCCCCCAATGACCCCCTGGACCTGGGCTACGAGGTGCAGATCTGC
  AGGTATAAAGACTACCGAGAGCCGCCGTGGTCGGAAAACAAGTACGACATCTCCAAGGAC
  TTCTGGGCCGTCCTGGCAGCCCGGCTGGCGTTTGTCATCGTCTTCCAGAACCTGGTCATG
  TTCATGAGCGACTTTGTGGACTGGGTCATCCCGGACATCCCCAAGGACATCAGCCAGCAG
  ATCCACAAGGAGAAGGTGCTCATGGTGGAGCTGTTCATGCGGGAGGAGCAAGACAAGCAG
  CAGCTGCTGGAAACCTGGATGGAGAAGGAGCGGCAGAAGGACGAGCCGCCGTGCAACCAC
  CACAACACCAAAGCCTGCCCAGACAGCCTCGGCAGCCCAGCCCCCAGCCATGCCTACCAC
  GGGGGCGTCCTGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_018043
• Insert Size: 5200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: It is not a varient.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018043.4, NP_060513.4
• RefSeq Size: 4811 bp
• RefSeq ORF: 2883 bp
• Locus ID: 55107
• UniProt ID: Q5XXA6
• Cytogenetics: 11q13.3
• Domains: DUF590
• Protein Families: Transmembrane
• Gene Summary: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.