SWI5 (NM_001040011) Human Untagged Clone

CAT#: SC316346

SWI5 (untagged)-Human chromosome 9 open reading frame 119 (C9orf119)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SWI5
• Synonyms: C9orf119; SAE3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316346 representing NM_001040011.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGCGGCGTGGCCAGAGGGACCTGTGGCGTCACAACAAAAGCTGCGCACGCAACCGCTGTCCCCGC
  CCACCTCGGGAGAGGGGCGGGGCCGGCTTTCCTTGGGTGCGCGCGCAGCTTTCTGTGCGCCAGTTCACA
  CTCCGGGTCAGAGTTCCTGGCCCGGTGCACCTGAGAGGTCGCTCTCCGACTCCCGCGCTGGACCCTCTT
  GCGCCATTGAACCCCCTGATCCGGGGGCCTCGGACCCCAGGGCTCAGGAGATGGATCCAGTCCCTGGCG
  CTCCTACTTCCCAACTGCTCCTCCTCCCGCATCCCCACAGTACCCCGTCCTCACAGCGGGCTGTGGGTC
  CAATCAGACTTTCCCCTCGGATTCCTCTCTAGGACTGAACCAAGACTTACCCGAAGTTGCCGCGGGGCC
  TTCCGATCCCCTAGGCCATTGCCCAAGTCTGGCCAGGCTGATGGGACCAGCGAGGAGTCTCTGCACCTT
  GACATTCAGAAACTGAAGGAGAAGAGGGACATGCTGGACAAGGAGATCTCCCAGTTCGTATCTGAAGGC
  TACAGTGTGGATGAACTGGAGGACCACATTACCCAGCTTCACGAGTACAATGACATCAAGGATGTGGGG
  CAGATGCTGATGGGCAAACTAGCTGTGATCCGAGGTGTCACCACCAAAGAGTTGTATCCAGAGTTTGGG
  CTGGACATGAATGACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001040011
• Insert Size: 708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040011.1
• RefSeq Size: 1004 bp
• RefSeq ORF: 708 bp
• Locus ID: 375757
• UniProt ID: Q1ZZU3
• Cytogenetics: 9q34.11
• MW: 26.7 kDa
• Gene Summary: Component of the SWI5-SFR1 complex, a complex required for double-strand break repair via homologous recombination.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).