NEMF (NM_004713) Human Untagged Clone

CAT#: SC315149

NEMF (untagged)-Human nuclear export mediator factor (NEMF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEMF
• Synonyms: IDDSAPN; NY-CO-1; SDCCAG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315149 representing NM_004713.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGAGCCGCTTTAGCACCATTGACCTCCGCGCCGTACTCGCGGAGCTGAATGCTAGCTTGCTAGGA
  ATGAGAGTAAACAATGTTTATGATGTGGATAATAAGACATACCTTATTCGTCTTCAAAAACCGGACTTT
  AAAGCTACACTTTTACTTGAATCTGGCATACGAATTCATACAACAGAATTTGAGTGGCCTAAGAATATG
  ATGCCGTCTAGTTTTGCCATGAAGTGCCGAAAACATTTGAAGAGTCGGAGATTAGTCAGTGCAAAACAG
  CTTGGTGTGGATAGAATTGTAGATTTTCAATTTGGAAGTGATGAAGCTGCTTACCATTTAATCATTGAG
  CTCTATGATAGGGGGAACATTGTTCTTACAGATTATGAGTACGTAATTTTAAATATTCTAAGGTTTCGA
  ACTGATGAGGCAGATGATGTTAAATTTGCTGTTCGTGAACGCTATCCACTTGATCATGCTAGAGCTGCT
  GAACCTTTGCTTACTTTGGAAAGGTTGACTGAAATAGTAGCCAGCGCACCTAAGGGTGAACTACTGAAG
  AGGGTGCTTAACCCATTACTTCCCTATGGACCAGCTCTCATTGAACACTGTCTTTTAGAAAATGGATTC
  TCGGGTAATGTCAAAGTGGATGAAAAACTTGAAACTAAAGATATTGAAAAAGTACTTGTTTCTCTGCAG
  AAAGCAGAAGACTATATGAAAACAACATCCAACTTCAGTGGGAAGGGATATATCATTCAGAAAAGAGAA
  ATAAAACCATGCTTGGAAGCAGATAAACCAGTTGAAGACATACTGACGTATGAGGAATTTCATCCTTTC
  TTGTTTTCTCAACATTCACAATGTCCATATATAGAATTTGAATCATTTGACAAGGCGGTGGATGAATTT
  TATTCCAAGATAGAAGGCCAGAAAATTGACTTAAAAGCTTTACAACAGGAAAAGCAAGCATTGAAGAAA
  TTAGATAATGTTCGAAAGGATCACGAAAACAGATTGGAAGCTCTTCAGCAGGCTCAGGAAATAGACAAA
  CTGAAAGGAGAGCTCATAGAAATGAACCTACAAATAGTTGACAGAGCCATTCAGGTAGTTCGAAGTGCT
  TTAGCTAACCAGATAGATTGGACAGAAATTGGGTTAATTGTGAAAGAAGCCCAGGCTCAAGGAGACCCT
  GTTGCAAGTGCAATCAAAGAATTAAAACTACAAACAAACCATGTTACAATGCTGCTAAGAAATCCATAC
  TTGTTATCAGAGGAGGAAGATGATGATGTTGATGGTGACGTCAATGTTGAGAAAAATGAAACTGAACCA
  CCAAAAGGAAAAAAGAAAAAACAAAAGAATAAACAGCTGCAGAAGCCTCAGAAAAATAAGCCCTTACTT
  GTAGATGTTGATCTCAGCTTGTCAGCATATGCCAATGCCAAAAAGTATTATGATCACAAGAGATATGCT
  GCTAAGAAAACACAAAAGACTGTTGAAGCTGCTGAGAAGGCATTCAAGTCAGCAGAAAAGAAAACAAAG
  CAAACATTAAAAGAAGTTCAGACTGTTACCTCTATTCAAAAAGCAAGAAAAGTATATTGGTTTGAGAAA
  TTTCTGTGGTTCATTAGCTCAGAGAACTATCTAATTATAGGTGGACGAGATCAGCAACAGAATGAAATA
  ATTGTGAAAAGATACTTGACACCAGGAGACATTTATGTACATGCTGATCTTCATGGAGCTACTAGCTGT
  GTAATTAAGAATCCAACAGGAGAACCCATCCCCCCACGGACCTTGACTGAAGCTGGCACAATGGCACTT
  TGCTACAGTGCTGCTTGGGATGCACGAGTTATCACTAGTGCTTGGTGGGTGTACCATCATCAGGTATCT
  AAAACAGCACCAACTGGAGAATATTTGACAACAGGAAGCTTCATGATAAGAGGAAAAAAGAATTTTCTT
  CCTCCCTCATATCTAATGATGGGGTTTAGCTTCCTTTTTAAGGTAGATGAGTCTTGTGTTTGGAGACAT
  CAGGGTGAACGAAAAGTCAGAGTACAGGATGAAGACATGGAGACACTGGCAAGTTGTACAAGTGAACTC
  ATATCAGAAGAAATGGAACAATTAGATGGAGGTGACACGAGCAGTGATGAGGATAAAGAAGAACATGAA
  ACTCCTGTGGAAGTAGAACTCATGACTCAGGTTGACCAAGAGGATATCACTCTTCAGAGTGGCAGAGAT
  GAACTAAATGAGGAGCTCATTCAGGAAGAAAGCTCTGAAGACGAAGGAGAATATGAAGAGGTTAGAAAA
  GATCAGGATTCTGTTGGTGAAATGAAGGATGAAGGGGAAGAGACATTAAATTATCCTGATACTACCATT
  GACTTGTCTCACCTTCAACCCCAAAGGTCCATCCAGAAATTGGCTTCAAAAGAGGAATCTTCTAATTCT
  AGTGACAGTAAATCACAGAGCCGGAGACATTTGTCAGCCAAGGAAAGAAGGGAAATGAAAAAGAAAAAA
  CTTCCAAGTGACTCAGGAGATTTAGAAGCGTTAGAGGGAAAGGATAAAGAAAAAGAAAGTACTGTACAC
  ATTGAAACTCATCAGAACACAAGCAAAAATGTTGCGGCTGTGCAGCCAATGAAACGAGGACAAAAGAGT
  AAAATGAAAAAAATGAAAGAAAAATACAAAGACCAGGATGAAGAAGACCGTGAACTTATCATGAAGTTG
  CTGGGGTCTGCAGGTTCAAACAAAGAAGAAAAAGGGAAGAAGGGGAAGAAAGGAAAAACAAAGGACGAA
  CCTGTGAAGAAACAGCCCCAGAAACCTAGAGGTGGACAGAGGGTCTCTGACAACATTAAGAAAGAAACT
  CCGTTCCTTGAGGTTATAACTCATGAGTTACAAGACTTTGCTGTAGATGATCCACATGATGACAAGGAA
  GAGCAAGATCTGGATCAACAGGGAAATGAGGAAAACCTATTTGATTCTTTGACAGGCCAGCCACATCCT
  GAAGATGTACTACTGTTTGCCATTCCAATATGTGCCCCTTACACCACCATGACAAACTACAAATATAAA
  GTGAAACTTACTCCTGGAGTGCAGAAAAAGGGAAAAGCTGCAAAAACAGCCTTGAATAGTTTCATGCAT
  TCCAAAGAAGCAACAGCAAGAGAAAAAGACTTATTCCGCAGCGTAAAGGACACAGATTTATCAAGAAAC
  ATTCCTGGCAAAGTGAAAGTGTCTGCACCCAATCTTCTGAACGTAAAAAGGAAATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004713
• Insert Size: 3231 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004713.2
• RefSeq Size: 4396 bp
• RefSeq ORF: 3231 bp
• Locus ID: 9147
• UniProt ID: O60524
• Cytogenetics: 14q21.3
• MW: 123 kDa
• Gene Summary: This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).