PDZD6 (INTU) (NM_015693) Human Untagged Clone

CAT#: SC314961

INTU (untagged)-Human inturned planar cell polarity effector homolog (Drosophila) (INTU)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDZD6
• Synonyms: CPLANE4; INT; OFD17; PDZD6; PDZK6; SRTD20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314961 representing NM_015693.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCTCTGTGGCTTCGTGCGATTCGCGTCCGAGCTCAGACGAGCTCCCTGGAGACCCCTCTTCACAA
  GAAGAAGATGAGGACTATGATTTTGAAGATCGGGTCAGCGACTCGGGTTCATATTCCTCAGCGAGTAGC
  GATTATGATGATCTTGAGCCTGAATGGCTGGACAGTGTGCAGAAAAATGGAGAGCTGTTTTATTTGGAA
  TTGAGTGAGGATGAAGAAGAAAGCCTCCTTCCTGAGACACCAACTGTGAACCATGTCAGGTTCAGTGAA
  AATGAGATTATCATTGAAGATGACTACAAAGAAAGAAAAAAGTATGAACCCAAACTCAAGCAGTTTACC
  AAAATTTTAAGAAGGAAAAGACTTTTACCCAAGCGCTGCAATAAAAAAAATAGCAATGACAATGGACCA
  GTATCCATTCTAAAGCATCAGTCCAATCAGAAGACAGGAGTCATTGTCCAACAGCGATACAAAGATGTG
  AATGTTTATGTAAACCCCAAAAAGCTAACTGTTATCAAAGCCAAAGAGCAGCTCAAGCTTCTGGAAGTG
  CTGGTTGGAATTATTCATCAGACCAAGTGGAGCTGGAGAAGAACCGGAAAGCAGGGTGATGGAGAGAGG
  CTTGTGGTTCATGGCCTGCTGCCAGGGGGATCTGCTATGAAGAGCGGTCAGGTACTCATTGGTGATGTC
  CTTGTTGCTGTGAATGATGTCGATGTTACTACTGAAAACATCGAGAGAGTTCTGTCTTGCATTCCTGGA
  CCTATGCAGGTGAAACTGACATTTGAAAATGCATATGATGTGAAAAGGGAGACGTCCCATCCAAGACAG
  AAAAAGACACAGTCCAACACAAGTGATTTAGTCAAGCTTCTCTGGGGAGAAGAGGTTGAAGGTATCCAG
  CAGAGTGGCCTAAACACTCCTCATATCATTATGTATCTCACACTACAGCTCGACTCAGAAACCTCAAAG
  GAAGAGCAGGAAATTCTTTATCATTATCCAATGTCTGAAGCATCTCAGAAACTTAAAAGTGTGAGAGGG
  ATTTTTCTCACACTCTGTGACATGCTGGAAAACGTAACTGGGACACAAGTTACTAGTTCATCCCTCCTT
  TTAAATGGAAAACAAATTCATGTGGCTTATTGGAAAGAATCTGACAAGTTGTTGCTAATTGGCCTGCCT
  GCTGAAGAAGTTCCTCTTCCTCGTCTAAGGAACATGATAGAAAATGTCATCCAAACCTTAAAATTTATG
  TATGGTTCTTTAGATAGTGCCTTTTGCCAGATTGAGAATGTTCCTCGTTTGGATCATTTTTTTAACTTG
  TTCTTTCAAAGAGCACTTCAGCCTGCGAAACTGCATTCCAGCGCCAGTCCCAGTGCTCAGCAGTACGAT
  GCTTCCAGTGCAGTACTTTTAGACAACCTCCCTGGAGTCCGGTGGCTCACACTTCCACTGGAAATCAAG
  ATGGAATTAGACATGGCATTAAGTGACTTGGAGGCTGCAGATTTTGCAGAACTGTCCGAGGATTACTAT
  GACATGAGGCGGCTGTATACAATTTTGGGGTCTTCTCTATTTTACAAGGGTTATTTGATATGCAGTCAT
  TTGCCCAAGGATGATCTTATTGATATTGCCGTATACTGTCGCCACTATTGCCTGCTGCCTTTAGCAGCA
  AAACAAAGAATTGGTCAGTTGATCATATGGAGAGAAGTGTTTCCTCAGCATCACCTCCGACCTTTGGCA
  GACTCAAGCACTGAAGTCTTTCCGGAACCTGAAGGAAGATATTTTTTGCTAGTTGTTGGCTTGAAACAT
  TATATGCTATGTGTACTATTAGAAGCTGGAGGTTGCGCATCCAAAGCTATTGGGAGTCCTGGACCAGAC
  TGTGTATATGTGGATCAAGTCAAAACAACTCTTCACCAGCTGGATGGAGTAGATTCTCGCATAGATGAA
  CGGCTAGCATCTTCTCCAGTCCCCTGTTTGTCTTGTGCTGACTGGTTCCTTACTGGATCACGTGAAAAA
  ACAGATAGCTTGACCACTTCGCCTATTCTCAGTAGGCTACAAGGTACTTCCAAAGTAGCAACTTCTCCA
  ACATGCAGAAGAACGCTTTTTGGTGACTATTCCTTAAAGACACGCAAGCCTAGTCCTTCCTGTAGTAGT
  GGAGGATCTGACAATGGTTGTGAAGGTGGAGAAGATGATGGCTTTAGCCCCCATACTACACCGGATGCA
  GTACGGAAGCAAAGAGAATCTCAGGGCTCTGATGGTTTAGAAGAAAGTGGGACCTTGCTTAAGGTCACT
  AAAAAGAAGTCTACTCTTCCAAATCCATTTCATTTGGGAAACTTGAAAAAGGACCTTCCAGAAAAAGAA
  TTAGAAATATATAACACAGTGAAACTGACATCTGGTCCTGAGAACACACTTTTCCACTACGTTGCCTTA
  GAAACAGTGCAAGGAATCTTTATTACTCCTACCCTTGAAGAGGTGGCACAGCTAAGTGGCTCTATCCAC
  CCTCAGCTAATAAAGAATTTCCATCAGTGTTGTCTTTCCATTCGTGCAGTTTTCCAACAGACATTGGTG
  GAAGAGAAAAAGAAAGGACTAAATAGTGGAGACCATTCAGATTCTGCAAAGTCAGTGTCTTCTCTTAAC
  CCTGTTAAAGAACATGGTGTGTTGTTTGAATGTTCACCTGGAAACTGGACTGATCAGAAAAAAGCACCA
  CCAGTTATGGCTTACTGGGTAGTAGGGAGACTTTTTCTTCATCCAAAACCTCAAGAACTTTATGTCTGT
  TTTCATGACTCAGTCACAGAAATTGCCATTGAAATAGCTTTTAAATTGTTCTTTGGGTTAACCTTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015693
• Insert Size: 2829 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015693.3
• RefSeq Size: 3275 bp
• RefSeq ORF: 2829 bp
• Locus ID: 27152
• UniProt ID: Q9ULD6
• Cytogenetics: 4q28.1
• MW: 105.6 kDa
• Gene Summary: Plays a key role in ciliogenesis and embryonic development. Regulator of cilia formation by controlling the organization of the apical actin cytoskeleton and the positioning of the basal bodies at the apical cell surface, which in turn is essential for the normal orientation of elongating ciliary microtubules. Plays a key role in definition of cell polarity via its role in ciliogenesis but not via conversion extension. Has an indirect effect on hedgehog signaling (By similarity). Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies (PubMed:27158779).[UniProtKB/Swiss-Prot Function]