APRIL (TNFSF13) (NM_003808) Human Untagged Clone

CAT#: SC312642

TNFSF13 (untagged)-Human tumor necrosis factor (ligand) superfamily, member 13 (TNFSF13), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APRIL
• Synonyms: APRIL; CD256; TALL-2; TALL2; TNLG7B; TRDL-1; UNQ383/PRO715; ZTNF2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003808 edited
  ATGCCAGCCTCATCTCCTTTCTTGCTAGCCCCCAAAGGGCCTCCAGGCAACATGGGGGGC
  CCAGTCAGAGAGCCGGCACTCTCAGTTGCCCTCTGGTTGAGTTGGGGGGCAGCTCTGGGG
  GCCGTGGCTTGTGCCATGGCTCTGCTGACCCAACAAACAGAGCTGCAGAGCCTCAGGAGA
  GAGGTGAGCCGGCTGCAGAGGACAGGAGGCCCCTCCCAGAATGGGGAAGGGTATCCCTGG
  CAGAGTCTCCCGGAGCAGAGTTCCGATGCCCTGGAAGCCTGGGAGAGTGGGGAGAGATCC
  CGGAAAAGGAGAGCAGTGCTCACCCAAAAACAGAAGAAGCAGCACTCTGTCCTGCACCTG
  GTTCCCATTAACGCCACCTCCAAGGATGACTCCGATGTGACAGAGGTGATGTGGCAACCA
  GCTCTTAGGCGTGGGAGAGGCCTACAGGCCCAAGGATATGGTGTCCGAATCCAGGATGCT
  GGAGTTTATCTGCTGTATAGCCAGGTCCTGTTTCAAGACGTGACTTTCACCATGGGTCAG
  GTGGTGTCTCGAGAAGGCCAAGGAAGGCAGGAGACTCTATTCCGATGTATAAGAAGTATG
  CCCTCCCACCCGGACCGGGCCTACAACAGCTGCTATAGCGCAGGTGTCTTCCATTTACAC
  CAAGGGGATATTCTGAGTGTCATAATTCCCCGGGCAAGGGCGAAACTTAACCTCTCTCCA
  CATGGAACCTTCCTGGGGTTTGTGAAACTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003808 unedited
  NGGTTAGAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGTTCCTCCTG
  GGTGTCACTGGCAGCCCTGTCCTTCCTAGAGGACTGGAACCTAATTCTCCTGAGGCTGAG
  GGAGGGTGGAGGGTCTCAAGGCAACGCTGGCCCCACGACGGAGTGCCAGGAGCACTAACA
  GTACCCTTAGCTTGCTTTCCTCCTCCCTCCTTTTTATTTTCAAGTTCCTTTTTATTTCTC
  CTTGCGTAACAACCTTCTTCCCTTCTGCACCACTGCCCGTACCCTTACCCGCCCCGCCAC
  CTCCTTGCTACCCCACTCTTGAAACCACAGCTGTTGGCAGGGTCCCCAGCTCATGCCAGC
  CTCATCTCCTTTCTTGCTAGCCCCCAAAGGGCCTCCAGGCAACATGGGGGGCCCAGTCAG
  AGAGCCGGCACTCTCAGTTGCCCTCTGGTTGAGTTGGGGGGCAGCTCTGGGGGCCGTGGC
  TTGTGCCATGGCTCTGCTGACCCAACAAACAGAGCTGCAGAGCCTCAGGAGAGAGGTGAG
  CCGGCTGCAGAGGACAGGAGGCCCCTCCCAGAATGGGGAAGGGTATCCCTGGCAGAGTCT
  CCCGGAGCAGAGTTCCGATGCCCTGGAAGCCTGGGAGAGTGGGGAGAGATCCCGGAAAAG
  GAGAGCAGTGCTCACCCAAAAACAGAAGAAGCAGCACTCTGTCCCTGCACTGGTTCCCCA
  TTACCGCCCACCTCCAGGATGACTCCGATGTGACAGAGGTGATGTGGCAACCANCTCTTA
  CGCGTGGCAGAAGCCCTCAGGCCCCAAGAATATGTGTTCCGATCCAGGATGCTGGNAGTT
  ATCTGCTGTATACCACGGCCCTGTTCCAGACGTGACTTTCACCTGGGTCCAGTGGTGGCT
  CGAGAAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003808 unedited
  CGTTGCGGTTGTTCACCTTGCTAGGACGCGGNGNCGAGCACTGTGGGNAGCGGGTCACAG
  GGNATGCCACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGA
  GTTTTTTTTTTTTTTTTTTTTTATCATTCATTCTATTTTATTTAACTTTAAGCAGGGCTT
  GATCAGAAAGAAGAGCTCAGAGGAGTGAGGCAACACCAACACCAGGGCGTACGATACACC
  CGAGGCTCCTCCGACACCAGTTCCAGCCCGGAAGGGCTTGGAATGGCCTGCCACGCCGGG
  GCAATGGAGCGCGTGGAAAGCCAAACCTTGGGACCCGAAGAGTAATTTGACTGCGAAGGC
  ACCGTCATCGTCGGGGCACCCCCTGTGGACGGGCGCATAATAGCTCCATCTCTGGAGATA
  GTGATGGAAACTGGGGTCCGATAGATGGAGAGAAGGACGTAGGATAAGGCTTCTTGTGGG
  GTGAAGAGGCATAGGGCACCTCGGAAGAGGTGTGGCATGGGAGAACCAGGGGTTTTCCCT
  AACAAAGGGCGGCTTCCTGGACTTCGCCAACCGGCGGGTGCTTTCCTCGAGCGGCCGTTA
  CAGGGGGGGGCTGTTCTGGATGCTTCCAGTGGGCCCCGGACCAGGTCTGGGGCNATGCGT
  GCGGGCCGGGTCCCCCGCCCCTTATTTTATACTGGCGGGAAGAATTCCGGAGCCTCCTTG
  GGGAACAAGGAAGCGAGGATTGTCGTGATATCCAAAGTTCTAGCGGCTGGGGCAATAGAA
  AAAGGCGAAAGATGGGTGAACCGGGGGGGCATACCCTAGGGAAAGAGCTCTCGGGACTCC
  CGGACACTGGTCCGCGCCCTTTAAAAATCCAAGGTCTTTGGCTGTGCTTCCAGAGTGGGG
  ACTGACCCCTGGTTCGTCAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003808
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003808.2, NP_003799.1
• RefSeq Size: 2276 bp
• RefSeq ORF: 753 bp
• Locus ID: 8741
• UniProt ID: O75888
• Cytogenetics: 17p13.1
• Domains: TNF
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• Gene Summary: The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]
  Transcript Variant: This variant (alpha) represents the longest transcript and encodes the longest isoform (alpha).