ACK1 (TNK2) (NM_001010938) Human Untagged Clone

CAT#: SC311417

TNK2 (untagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACK1
• Synonyms: ACK; ACK-1; ACK1; p21cdc42Hs
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001010938 edited
  ATGCTCGAGGCCCGGCCCCCCAGGACGCAGGGCAGTGACGCTGCCGGTGCCGCTGCGGGG
  CGGGGGCTGCGGGCGCTGCTTCTCTCCCTGACCGCAGCCGCTGGGATTTGGGGCTCCATG
  GGGGAGAGATCTGCTTACCAGCGCCTGGCTGGGGGCGAGGAGGGACCGCAGAGGCTGGGA
  GGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTGTCCGAGGTG
  CAGCTGCAACAGTACTTCCTGCGGCTCCGAGACGACCTCAACGTCACCCGCCTGTCCCAC
  TTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCCTGGCCAGCGG
  CGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCGTGGATGAGT
  AAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTTCCCACCTCATCACTCTCAGAGCACC
  TTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTGCAGAGCCTC
  ACCTGCCTCATTGGGGAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGATGGTTCCTTT
  GGCGTGGTGCGCAGGGGCGAGTGGGACGCGCCCTCAGGGAAGACGGTGAGTGTGGCTGTG
  AAGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCATGGACGACTTCATCCGGGAG
  GTCAATGCCATGCACTCGCTCGACCACCGAAACCTCATCCGCCTCTACGGGGTGGTGCTC
  ACGCCGCCCATGAAGATGGTGACAGAGCTGGCACCTCTGGGATCGTTGTTGGACCGGCTA
  CGTAAGCACCAGGGCCACTTCCTCCTGGGGACTCTGAGCCGCTACGCTGTGCAGGTGGCT
  GAGGGCATGGGCTACCTGGAGTCCAAGCGCTTTATTCACCGTGACCTGGCTGCCCGCAAT
  CTGCTGTTGGCTACCCGCGACCTGGTCAAGATCGGGGACTTTGGGCTGATGCGAGCACTA
  CCTCAGAATGACGACCATTACGTCATGCAGGAACATCGCAAGGTGCCCTTCGCCTGGTGT
  GCCCCCGAGAGCCTGAAGACACGTACCTTCTCCCATGCCAGCGACACCTGGATGTTCGGG
  GTGACACTGTGGGAAATGTTCACCTACGGCCAGGAGCCCTGGATCGGCCTCAACGGCAGT
  CAGATCCTGCATAAGATCGACAAGGAGGGGGAGCGGCTGCCCCGGCCCGAGGACTGTCCC
  CAGGACATCTACAACGTCATGGTCCAGTGCTGGGCTCACAAGCCAGAGGACAGACCCACG
  TTTGTGGCCCTGCGGGACTTCCTGCTGGAGGCCCAGCCCACAGACATGCGGGCCCTTCAG
  GACTTTGAGGAACCGGACAAGCTGCACATCCAGATGAATGATGTCATCACCGTCATCGAG
  GGAAGGGCCGAGAACTACTGGTGGCGTGGCCAGAACACACGGACGCTGTGTGTGGGGCCC
  TTCCCTCGCAACGTGGTGACCTCCGTGGCCGGCCTGTCGGCCCAGGACATCAGCCAGCCC
  CTGCAGAACAGCTTCATCCACACAGGGCATGGCGACAGTGACCCCCGCCACTGCTGGGGC
  TTCCCGGACAGGATTGACGAACTGTATCTGGGAAACCCCATGGACCCCCCCGACCTCCTG
  AGCGTGGAACTGAGCACCTCCCGGCCCCCCCAGCATCTAGGAGGGGTGAAAAAACCAACC
  TATGACCCTGTGAGCGAGGACCAAGACCCCTTGTCCAGCGACTTCAAGAGGCTGGGCCTG
  CGGAAGCCAGGCCTGCCCCGAGGGCTGTGGCTGGCGAAGCCCTCGGCGCGGGTGCCGGGC
  ACCAAGGCCAGCCGAGGCAGCGGGGCTGAGGTCACGCTCATCGACTTCGGTGAGGAGCCC
  GTGGTCCCGGCCCTACGGCCCTGCGCGCCCTCCCTGGCGCAGCTGGCCATGGACGCCTGC
  TCCCTGCTGGACGAGACCCCGCCTCAGAGCCCCACGCGGGCACTGCCCCGGCCCCTGCAC
  CCCACGCCTGTGGTGGACTGGGACGCACGCCCGCTGCCCCCCCCGCCCGCCTATGACGAC
  GTGGCCCAGGATGAGGATGACTTTGAGATCTGCTCCATCAACAGCACCCTCGTGGGCGCG
  GGGGTCCCTGCCGGGCCCAGCCAGGGCCAGACCAACTACGCCTTTGTGCCTGAGCAGGCG
  CGGCCGCCCCCTCCCCTGGAGGACAACCTGTTCCTCCCGCCCCAGGGTGGGGGCAAGCCG
  CCCAGCTCCGCACAGACCGCAGAGATCTTCCAGGCGCTACAGCAGGAGTGCATGAGGCAA
  CTGCAGGCTCCGGCCGGCTCCCCGGCCCCCTCTCCCAGCCCGGGGGGTGACGACAAGCCC
  CAGGTGCCTCCTCGGGTACCCATCCCCCCTCGGCCCACGCGCCCACACGTCCAGCTGTCT
  CCAGCCCCCCCGGGCGAGGAGGAGACCAGCCAGTGGCCTGGACCTGCTTCCCCTCCCCGG
  GTGCCTCCGCGGGAGCCCCTGTCCCCTCAAGGCTCGAGGACACCCAGCCCCCTGGTACCA
  CCTGGCAGCTCCCCGCTGCCACCCCGGCTCTCAAGCTCACCTGGGAAGACCATGCCCACC
  ACCCAGAGCTTTGCCTCAGACCCCAAGTACGCCACCCCCCAGGTGATCCAGGCCCCTGGC
  CCGCGGGCTGGTCCCTGCATCCTGCCCATCGTCCGGGATGGCAAGAAGGTCAGCAGCACC
  CACTATTACTTGCTGCCCGAGCGACCATCCTACCTGGAGCGCTACCAGCGCTTCCTGCGT
  GAGGCCCAGAGCCCCGAGGAGCCTACCCCCCTGCCTGTGCCTCTGCTGCTGCCCCCACCC
  AGCACCCCAGCCCCCGCCGCCCCCACGGCCACCGTGCGGCCGATGCCCCAGGCTGCCTTG
  GACCCCAAGGCCAACTTCTCCACCAACAACAGCAACCCAGGGGCCCGGCCACCACCCCCG
  AGGGCCACTGCTCGGCTGCCACAGAGGGGCTGCCCTGGCGATGGGCCAGAGGCGGGCCGG
  CCAGCAGACAAGATCCAGATGCTGCAGGCCATGGTGCATGGGGTGACCACAGAGGAGTGC
  CAGGCGGCCCTGCAGTGCCACGGCTGGAGCGTGCAGAGGGCTGCCCAGTATCTGAAGGTG
  GAGCAGCTCTTCGGGCTGGGTCTGCGGCCCAGAGGGGAGTGCCACAAAGTGCTGGAGATG
  TTCGACTGGAACCTGGAGCAGGCCGGCTGCCACCTTCTGGGCTCCTGGGGCCCTGCCCAC
  CACAAGCGCTGAGATGCGTCTGGAGAGCCAGAGGGCCTGCCTGAAGGAATCACCTGAGCC
  TGTCCGTCCACCAGGAGTGGGGAGATGCCCCCATCCAGTCCTGGAGGACCCGCTGCTCCT
  GCTGCTCCCGGGGATGGAGCAAGGCCAAGGCTGCGGGAGGCTGGGAGCCCTGCCCTGCCC
  ATCCCTCCTGCACCAGCGCTGTCCCTGCACACTTTGGTTCAGTCCCGGTGCCCCTGCCAA
  GATGTGGAAGGGGCCGGGTGAAGACAGGCTTGAGGGCTGCCCCAGCAGGCTCTGGGTATG
  ACCTGCCTCTGGCCCTGGTCCTGGGCGGGGCCTGTGGGTGGAGTAGTACCCCCAGGCCCT
  GCCCTGGGTGACAGACTGGGAGGAAACCAGGCTGGACCTGGGCAGGCGGGATGTGTTGGC
  CACAGGGAGAGGCGGACCGGCACCCGGTGGGACCTCCTAGGACTGGGCCTTCTTCCAGGG
  GGCCCCTGGCAGCAGCTGGGGTGTCGGGCAGAATGTGACTTGTGGCCTTCCCCAGCCTGT
  TGATGAAGTTGCTGTTGCCTGCATCTAGTACGTGTTGTTCCACAGAAATAAAGTTAGTTT
  GTATTTTATGTTAAAACAAAACAAATAAAACTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001010938 unedited
  GATCAAACTCCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC
  CGCGAATTCATGCTCGAGGCCCGGCCCCCCAGGACGCAGGGCAGTGACGCTGCCGGTGCC
  GCTGCGGGGCGGGGGCTGCGGGCGCTGCTTCTCTCCCTGACCGCAGCCGCTGGGATTTGG
  GGCTCCATGGGGGAGAGATCTGCTTACCAGCGCCTGGCTGGGGGCGAGGAGGGACCGCAG
  AGGCTGGGAGGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTG
  TCCGAGGTGCAGCTGCAACAGTACTTCCTGCGGCTCCGAGACGACCTCAACGTCACCCGC
  CTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCCT
  GGCCAGCGGCGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCG
  TGGATGAGTAAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTTCCCACCTCATCACTCT
  CAGAGCACCTTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTG
  CAGAGCCTCACCTGCCTCATTGGNGAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGAT
  GGTTCCTTTGCCGTTGTGCGCAGGGGCAAGTGGGACGCGCCCTCAGGGAAGACGGTGAGT
  GTGGCTGTGAAGTGCCTGAAGCCCCGATGTCTGGAGCAACCCAGAGCCATGGACAATTTA
  TTCGGGGAGGTCATGGCATGCACTCGCTCGACCACCGAACCCTTTTCGGCTTTAGGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001010938
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: It is not a varient.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010938.1, NP_001010938.1
• RefSeq Size: 4222 bp
• RefSeq ORF: 3261 bp
• Locus ID: 10188
• UniProt ID: Q07912
• Cytogenetics: 3q29
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has a distinct 5' UTR and 5' coding region, lacks one alternate 3' coding exon, and contains a different alternate 3' coding exon, as compared to variant 1. The translation remains in-frame, and results in a longer isoform (2) that has a longer and distinct N-terminus, compared to isoform 1. CCDS Note: The coding region has been updated to start at a downstream in-frame start codon that is supported by conservation data.