FEZ2 (NM_001042548) Human Untagged Clone

CAT#: SC311373

FEZ2 (untagged)-Human fasciculation and elongation protein zeta 2 (zygin II) (FEZ2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FEZ2
• Synonyms: HUM3CL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC311373 representing NM_001042548.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCGGACGGGGACTGGCAGGATTTCTATGAGTTCCAGGAGCCGGCCCGGAGCCTCCTGGACCAG
  GAGAACTGTAACGCGAGCCCCGAGCCTGGGGCGGAGGCGGGGGCCGAGGCGGGTGGGGGCGCCGACGGT
  TTCCCGGCCCCGGCCTGCAGCTTGGAGGAGAAGCTGAGCCTGTGCTTCCGCCCCTCGGATCCGGGCGCC
  GAGCCCCCGAGGACGGCCGTGCGGCCCATCACGGAGCGCAGCCTCCTGCAGGGGGACGAGATTTGGAAT
  GCCCTGACAGATAATTATGGGAATGTGATGCCTGTAGACTGGAAGTCATCGCATACTAGGACCTTGCAC
  TTGCTTACTCTGAACCTCTCAGAAAAAGGGGTAAGTGACAGTTTGCTCTTTGATACATCAGATGATGAA
  GAGCTGAGAGAACAGCTGGATATGCACTCAATCATCGTCTCCTGTGTTAATGATGAACCCCTCTTCACG
  GCAGACCAGGTTATTGAAGAAATTGAAGAAATGATGCAGGAATCACCGGACCCAGAAGATGATGAAACC
  CCTACACAGTCAGATCGGCTTTCAATGCTTTCCCAGGAAATTCAAACTCTCAAGAGGTCTAGTACCGGC
  AGTTATGAAGAGAGAGTGAAAAGGCTCTCAGTGTCTGAGTTAAATGAAATCCTGGAAGAAATTGAGACT
  GCCATTAAGGAGTACTCTGAGGAGCTGGTGCAGCAGTTGGCTTTACGAGATGAACTGGAGTTTGAAAAG
  GAAGTGAAAAACAGCTTTATTTCTGTTCTTATTGAAGTGCAAAACAAACAGAAAGAGCACAAAGAAACA
  GCAAAAAAGAAAAAGAAACTAAAAAATGGCAGCTCTCAGAATGGGAAGAATGAGAGAAGTCATATGCCC
  GGCACACGCTTCAGCATGGAAGGGATCTCAAATGTCATTCAGAATGGCCTCCGCCACACCTTTGGAAAT
  TCAGGTGGAGAGAAACAGTATTTGACTACAGTCATTCCTTATGAGAAAAAAAACGGACCACCGTCTGTT
  GAAGATCTTCAAATATTAACAAAAATTCTTCGTGCCATGAAGGAGGACAGTGAAAAAGTTCCGAGCTTG
  TTAACTGATTATATTCTGAAAGTTCTGTGTCCTACATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042548
• Insert Size: 1143 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042548.1
• RefSeq Size: 2110 bp
• RefSeq ORF: 1143 bp
• Locus ID: 9637
• UniProt ID: Q9UHY8
• Cytogenetics: 2p22.2
• MW: 42.6 kDa
• Gene Summary: This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Other orthologs include the rat gene that encodes zygin II, which can bind to synaptotagmin. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) includes an alternate in-frame exon, compared to variant 1, resulting in a longer protein isoform.