FBXO44 (NM_033182) Human Untagged Clone

CAT#: SC311243

FBXO44 (untagged)-Human F-box protein 44 (FBXO44), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXO44
• Synonyms: FBG3; FBX6A; FBX30; Fbx44; Fbxo6a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_033182 edited
  ATGGCTGTGGGGAACATCAACGAGCTGCCCGAGAACATCCTGCTGGAGCTGTTCACGCAC
  GTGCCCGCCCGCCAGCTGCTGCTGAACTGCCGCCTGGTCTGCAGCCTCTGGCGGGACCTC
  ATCGACCTCGTGACCCTCTGGAAACGCAAGTGCCTGCGAGAGGGCTTCATCACTGAGGAC
  TGGGACCAGCCCGTGGCCGACTGGAAGATCTTCTACTTCTTACGGAGCCTGCACAGGAAC
  CTCCTGCACAACCCGTGCGCTGAAGAGGGGTTCGAGTTCTGGAGCCTGGATGTGAATGGA
  GGCGATGAGTGGAAGGTGGAGGATCTCTCTCGAGACCAGAGGAAGGAATTCCCCAATGAC
  CAGGTCAAGAAATACTTCGTTACTTCATATTACACCTGCCTCAAGTCCCAGGTGGTGGAC
  CTCAAGGCCGAAGGGTATTGGGAGGAGCTGATGGATACCACACGGCCGGACATCGAGGTC
  AAGGACTGGTTCGCAGCCAGGCCAGATTGCGGGTCCAAGTACCAGCTGTGCGTTCAGCTC
  CTGTCGTCCGCGCACGCGCCTCTGGGGACCTTCCAGCCAGACCCGGCGACCATCCAGCAG
  AAGAGCGATGCCAAGTGGAGGGAGGTCTCCCACACATTCTCCAACTACCCGCCCGGCGTC
  CGCTACATCTGGTTTCAGCACGGCGGCGTGGACACTCATTACTGGGCCGGCTGGTACGGC
  CCGAGGGTCACCAACAGCAGCATCACCATCGGGCCCCCGCTGCCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033182 unedited
  GTTTACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGCAGGTCCA
  GGCGGTGCAGCTTGGGCGGCCCAGCGGATCGTGCCGCGGCGGCCGAGCGCAGTACAGGAG
  GGTGTCCAGAAGCCACAAGCCATGGCTGTGGGGAACATCAACGAGCTGCCCGAGAACATC
  CTGCTGGAGCTGTTCACGCACGTGCCCGCCCGCCAGCTGCTGCTGAACTGCCGCCTGGTC
  TGCAGCCTCTGGCGGGACCTCATCGACCTCGTGACCCTCTGGAAACGCAAGTGCCTGCGA
  GAGGGCTTCATCACTGAGGACTGGGACCAGCCCGTGGCCGACTGGAAGATCTTCTACTTC
  TTACGGAGCCTGCACAGGAACCTCCTGCACAACCCGTGCGCTGAAGAGGGGTTCGAGTTC
  TGGAGCCTGGATGTGAATGGAGGCGATGAGTGGAAGGTGGAGGATCTCTCTCGAGACCAG
  AGGAAGGAATTCCCCAATGACCAGGTCAAGAAATACTTCGTTACTTCATATTACACCTGC
  CTCAAGTCCCAGGTGGTGGACCTCAAGGCCGAAGGGTATTGGGAGGAGCTGATGGATACC
  ACACGGCCGGACATCGAGGTCAAGGACTGGTTCGCAGCCAGGCCAGATTGCGGGTCCAAG
  TACCAGCTGTGCGTTCAGCTCCTGTCGTCCGCGCACGCGCCTCTGGNGACCTTCCAGCCA
  GACCCGGCGACCATCCAGCAGAAGAGCGATGCCAAGTGGAGGGAGGTCTCCCACACATTC
  TCCCACTACCCGCCCGGCGTCCGCTACATCTGGTTTCAGCACGGCGGCGTGGACACTCAT
  TACTGGGCCCGGCTGGTACGGCCCGAGG
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_033182 unedited
  GTCCGCGCAGCGCCNTGGGGACTTCCACCGAACCGCGACCATCCACAAAAGACGATGCCA
  AGTGGAGGGAGGTCTCCCACACATTCTCCAACTACCCGCCCGGCGTCCGCTACATCTGGT
  TTCAGCACGGCGGCGTGGACACTCATTACTGGGCCGGCTGGTACGGCCCGAGGGTCACCA
  ACAGCAGCATCACCATCGGGCCCCCGCTGCCCTGACACCCCCTGAGCCCCCATCTGCTGA
  ACCCTGACTGGTAAACAACTGCTGTCAGAAAAGGGCTGGGCTTGGGAAGGGGAGGTGGAG
  GCCAGGTGTCCCCAGACCTCTAACCCTTGCCCCTAGCAGCCTCTTCTTTGTGGAGCCTCT
  CAGTGTGGGCAGCCCTCGCATGCTGGGGTCGGGCCAGCTCTCCCCGAAAGGTCTTGACCT
  GAATGATGGCCGGGGAAGCCTGCGTGTGCCCCTTTCAGAGACGGAGCACCTGAGATGTGG
  GAGGTGCAGCATGTTCCCCTGGGCCCCTCAGAAAGTCGAGCTTGGAGGCCAGCCTGGATC
  TGTCTCTCCCTTCCCCTCCTGGGACCATTCTACCTGTGTTCTTTGACCCTCGGAGCAGGG
  ACAGGCAAGACAACTGGCAAGCTTGCAGCTGCCCTGATGGTGCANGTGCAGGGAGGTGAC
  CATGTAACTCTGACCAATCTGGGGAGTGGAGGGTGGGCTCATGGGCCGTGCTCTGCCCGT
  CTGCTGCTCCCAGTCTTTCGCTCTGCCTGCCTGCTCAGAAGAAGTGGCTTTGGCCCAGAG
  CTCANGCCGGGACTGAGATGGACAGACCCAGGGTGGGGTGGNGTCCCAGTCGGGTGTGGA
  CTGTCCTCACTGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_033182
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033182.5, NP_149438.2
• RefSeq Size: 2948 bp
• RefSeq ORF: 768 bp
• Locus ID: 93611
• UniProt ID: Q9H4M3
• Cytogenetics: 1p36.22
• Domains: F-box, FBA
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It is also a member of the NFB42 (neural F Box 42 kDa) family, similar to F-box only protein 2 and F-box only protein 6. Several alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 4, and 6 all encode the same isoform (1).