HEY1 (NM_001040708) Human Untagged Clone

SKU SC311179

HEY1 (untagged)-Human hairy/enhancer-of-split related with YRPW motif 1 (HEY1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: HEY1
• Synonyms: BHLHb31; CHF2; HERP2; HESR1; hHRT1; HRT-1; NERP2; OAF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001040708 edited; GAGGCAAAGCGTGAGCGGGATCAGTGTGTGCGGAACGCAAGCAGCCGAGAGCGGAGAGGC; GCCGCTGTAGTTAACTCCTCCCTGCCCGCCGCGCCGACCCTCCCCAGGAACCCCCAGGGA; GCCAGCATGAAGCGAGCTCACCCCGAGTACAGCTCCTCGGACAGCGAGCTGGACGAGACC; ATCGAGGTGGAGAAGGAGAGTGCGGACGAGAATGGAAACTTGAGTTCGGCTCTAGGTTCC; ATGTCCCCAACTACATCTTCCCAGATTTTGGCCAGAAAAAGACGGAGAGGAATAATTGAG; AAGCGCCGACGAGACCGGATCAATAACAGTTTGTCTGAGCTGAGAAGGCTGGTACCCAGT; GCTTTTGAGAAGCAGGTAATGGAGCAAGGATCTGCTAAGCTAGAAAAAGCCGAGATCCTG; CAGATGACCGTGGATCACCTGAAAATGCTGCATACGGCAGGAGGGAAAGGTTACTTTGAC; GCGCACGCCCTTGCTATGGACTATCGGAGTTTGGGATTTCGGGAATGCCTGGCAGAAGTT; GCGCGTTATCTGAGCATCATTGAAGGACTAGATGCCTCTGACCCGCTTCGAGTTCGACTG; GTTTCGCATCTCAACAACTACGCTTCCCAGCGGGAAGCCGCGAGCGGCGCCCACGCGGGC; CTCGGACACATTCCCTGGGGGACCGTCTTCGGACATCACCCGCACATCGCGCACCCGCTG; TTGCTGCCCCAGAACGGCCACGGGAACGCGGGCACCACGGCCTCACCCACGGAACCGCAC; CACCAGGGCAGGCTGGGCTCGGCACATCCGGAGGCGCCTGCTTTGCGAGCGCCCCCTAGC; GGCAGCCTCGGACCGGTGCTCCCTGTGGTCACCTCCGCCTCCAAACTGTCGCCGCCTCTG; CTCTCCTCAGTGGCCTCCCTGTCGGCCTTCCCCTTCTCTTTCGGCTCCTTCCACTTACTG; TCTCCCAATGCACTGAGCCCTTCAGCACCCACGCAGGCTGCAAACCTTGGCAAGCCCTAT; AGACCTTGGGGGACGGAGATCGGAGCTTTTTAAAGAACTGATGTAGAATGAGGGAGGGGA; AAGTTTAAAATCCCAGCTGGGCTGGACTGTTGCCAACATCACCTTAAAGTCGTCAGTAAA; AGTAAAAAGGAAAAAGGTACACTTTCAGATAATTTTTTTTTTAAAGACTAAAGGTTTGTT; GGTTTACTTTTATCTTTTTTAATGTTTTTTTCATCATGTCATGTATTAGCAGTTTTTAAA; AACTAGTTGTTAAATTTTGTTCAAGACATTAAATTGAAATAGTGAGTATAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001040708 unedited; CTACACATTTGTACACGACTTACTACTAGGGCGGCCGCGAACTCGGCACGAGGCAAAGCG; TGAGCGGGATCAGTGTGTGCGGAACGCAAGCAGCCGAGAGCGGAGAGGCGCCGCTGTAGT; TAACTCCTCCCTGCCCGCCGCGCCGACCCTCCCCAGGAACCCCCAGGGAGCCAGCATGAA; GCGAGCTCACCCCGAGTACAGCTCCTCGGACAGCGAGCTGGACGAGACCATCGAGGTGGA; GAAGGAGAGTGCGGACGAGAATGGAAACTTGAGTTCGGCTCTAGGTTCCATGTCCCCAAC; TACATCTTCCCAGATTTTGGCCAGAAAAAGACGGAGAGGAATAATTGAGAAGCGCCGACG; AGACCGGATCAATAACAGTTTGTCTGAGCTGAGAAGGCTGGTACCCAGTGCTTTTGAGAA; GCAGGTAATGGAGCAAGGATCTGCTAAGCTAGAAAAAGCCGAGATCCTGCAGATGACCGT; GGATCACCTGAAAATGCTGCATACGGCAGGAGGGAAAGGTTACTTTGACGCGCACGCCCT; TGCTATGGACTATCGGAGTTTGGGATTTCGGGAATGCCTGGCAGAAGTTGCGCGTTATCT; GAGCATCATTGAAGGACTAGATGCCTCTGACCCGCTTCGAGTTCGACTGGTTTCGCATCT; CAACAACTACGCTTCCCAGCGGGAAGCCGCGAGCGGCGCCCACGCGGGCCTCGGACACAT; TCCCTGGGGGACCGTCTTCGGACATCACCCGCACATCGCGCACCCGCTGTTGCTGCCCCA; GAACGGCCCACGGGAACGCGGGCACCACGGCCTCACCCACGGAACCCGCACCACCAGGGC; AGGCTGGGCTCGGCACATCCGGAGGCGCCTGCTTTGCGAGCGCCCCTAGCGCAGCCTCGA; CCGTGCTCCCTGTGGTCACCTCCGCCTCAAACTGTCGCCGCCTCTGCTCTCTCAGTGGCC; TCCCTGTCGGCTTCCCCTTTCTCTTTCGGCTCCTTCCACTA;
• Restriction Sites: Please inquire
• ACCN: NM_001040708
• Insert Size: 1400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001040708.1, NP_001035798.1
• RefSeq Size: 2331 bp
• RefSeq ORF: 927 bp
• Locus ID: 23462
• UniProt ID: Q9Y5J3
• Cytogenetics: 8q21.13
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2), also known as HESR1-12nt, uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in a longer protein (isoform b).