JAK3 (NM_000215) Human Untagged Clone

CAT#: SC311134

JAK3 (untagged)-Human Janus kinase 3 (JAK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: JAK3
• Synonyms: JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000215 edited
  CCGGGATCCCGGCTCTGCCCGCCCTTCGAAAGTCCAGGGTCCCCGCCCGCTAGGACTGAG
  GGGCTTTTTCTCTCTGTGCCCCAGGCAAGTTGCACTCATGGCACCTCCAAGTGAAGAGAC
  GCCCCTGATCCCTCAGCGTTCATGCAGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGT
  GCTGCTGCCCGCTCGGGGCCCCGGGCCCCCCCAGCGCCTATCTTTCTCCTTTGGGGACCA
  CTTGGCTGAGGACCTGTGCGTGCAGGCTGCCAAGGCCAGCGGCATCCTGCCTGTGTACCA
  CTCCCTCTTTGCTCTGGCCACGGAGGACCTGTCCTGCTGGTTCCCCCCGAGCCACATCTT
  CTCCGTGGAGGATGCCAGCACCCAAGTCCTGCTGTACAGGATTCGCTTTTACTTCCCCAA
  TTGGTTTGGGCTGGAGAAGTGCCACCGCTTCGGGCTACGCAAGGATTTGGCCAGTGCTAT
  CCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCCCAGCACCGCAGTGACCTGGTGAGTGG
  GCGCCTCCCCGTGGGCCTCAGTCTCAAGGAGCAGGGTGAGTGTCTCAGCCTGGCCGTGTT
  GGACCTGGCCCGGATGGCGCGAGAGCAGGCCCAGCGGCCGGGAGAGCTGCTGAAGACTGT
  CAGCTACAAGGCCTGCCTACCCCCAAGCCTGCGCGACCTGATCCAGGGCCTGAGCTTCGT
  GACGCGGAGGCGTATTCGGAGGACGGTGCGCAGAGCCCTGCGCCGCGTGGCCGCCTGCCA
  GGCAGACCGGCACTCGCTCATGGCCAAGTACATCATGGACCTGGAGCGGCTGGATCCAGC
  CGGGGCCGCCGAGACCTTCCACGTGGGCCTCCCTGGGGCCCTTGGTGGCCACGACGGGCT
  GGGGCTGCTCCGCGTGGCTGGTGACGGCGGCATCGCCTGGACCCAGGGAGAACAGGAGGT
  CCTCCAGCCCTTCTGCGACTTTCCAGAAATCGTAGACATTAGCATCAAGCAGGCCCCGCG
  CGTTGGCCCGGCCGGAGAGCACCGCCTGGTCACTGTTACCAGGACAGACAACCAGATTTT
  AGAGGCCGAGTTCCCAGGGCTGCCCGAGGCTCTGTCGTTCGTGGCGCTCGTGGACGGCTA
  CTTCCGGCTGACCACGGACTCCCAGCACTTCTTCTGCAAGGAGGTGGCACCGCCGAGGCT
  GCTGGAGGAAGTGGCCGAGCAGTGCCACGGCCCCATCACTCTGGACTTTGCCATCAACAA
  GCTCAAGACTGGGGGCTCACGTCCTGGCTCCTATGTTCTCCGCCGCAGCCCCCAGGACTT
  TGACAGCTTCCTCCTCACTGTCTGTGTCCAGAACCCCCTTGGTCCTGATTATAAGGGCTG
  CCTCATCCGGCGCAGCCCCACAGGAACCTTCCTTCTGGTTGGCCTCAGCCGACCCCACAG
  CAGTCTTCGAGAGCTCCTGGCAACCTGCTGGGATGGGGGGCTGCACGTAGATGGGGTGGC
  AGTGACCCTCACTTCCTGCTGTATCCCCAGACCCAAAGAAAAGTCCAACCTGATCGTGGT
  CCAGAGAGGTCACAGCCCACCCACATCATCCTTGGTTCAGCCCCAATCCCAATACCAGCT
  GAGTCAGATGACATTTCACAAGATCCCTGCTGACAGCCTGGAGTGGCATGAGAACCTGGG
  CCATGGGTCCTTCACCAAGATTTACCGGGGCTGTCGCCATGAGGTGGTGGATGGGGAGGC
  CCGAAAGACAGAGGTGCTGCTGAAGGTCATGGATGCCAAGCACAAGAACTGCATGGAGTC
  ATTCCTGGAAGCAGCGAGCTTGATGAGCCAAGTGTCGTACCGGCATCTCGTGCTGCTCCA
  CGGCGTGTGCATGGCTGGAGACAGCACCATGGTGCAGGAATTTGTACACCTGGGGGCCAT
  AGACATGTATCTGCGAAAACGTGGCCACCTGGTGCCAGCCAGCTGGAAGCTGCAGGTGGT
  CAAACAGCTGGCCTACGCCCTCAACTATCTGGAGGACAAAGGCCTGCCCCATGGCAATGT
  CTCTGCCCGGAAGGTGCTCCTGGCTCGGGAGGGGGCTGATGGGAGCCCGCCCTTCATCAA
  GCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGATGCTCACCGACAGGAT
  CCCCTGGGTGGCCCCCGAGTGTCTCCGGGAGGCGCAGACACTTAGCTTGGAAGCTGACAA
  GTGGGGCTTCGGCGCCACGGTCTGGGAAGTGTTTAGTGGCGTCACCATGCCCATCAGTGC
  CCTGGATCCTGCTAAGAAACTCCAATTTTATGAGGACCGGCAGCAGCTGCCGGCCCCCAA
  GTGGACAGAGCTGGCCCTGCTGATTCAACAGTGCATGGCCTATGAGCCGGTCCAGAGGCC
  CTCCTTCCGAGCCGTCATTCGTGACCTCAATAGCCTCATCTCTTCAGACTATGAGCTCCT
  CTCAGACCCCACACCTGGTGCCCTGGCACCTCGTGATGGGCTGTGGAATGGTGCCCAGCT
  CTATGCCTGCCAAGACCCCACGATCTTCGAGGAGAGACACCTCAAGTACATCTCACAGCT
  GGGCAAGGGCAACTTTGGCAGCGTGGAGCTGTGCCGCTATGACCCGCTAGGCGACAATAC
  AGGTGCCCTGGTGGCCGTGAAACAGCTGCAGCACAGCGGGCCAGACCAGCAGAGGGACTT
  TCAGCGGGAGATTCAGATCCTCAAAGCACTGCACAGTGATTTCATTGTCAAGTATCGTGG
  TGTCAGCTATGGCCCGGGCCGCCAGAGCCTGCGGCTGGTCATGGAGTACCTGCCCAGCGG
  CTGCTTGCGCGACTTCCTGCAGCGGCACCGCGCGCGCCTCGATGCCAGCCGCCTCCTTCT
  CTATTCCTCGCAGATCTGCAAGGGCATGGAGTACCTGGGCTCCCGCCGCTGCGTGCACCG
  CGACCTGGCCGCCCGAAACATCCTCGTGGAGAGCGAGGCACACGTCAAGATCGCTGACTT
  CGGCCTAGCTAAGCTGCTGCCGCTTGACAAAGACTACTACGTGGTCCGCGAGCCAGGCCA
  GAGCCCCATTTTCTGGTATGCCCCCGAATCCCTCTCGGACAACATCTTCTCTCGCCAGTC
  AGACGTCTGGAGCTTCGGGGTCGTCCTGTACGAGCTCTTCACCTACTGCGACAAAAGCTG
  CAGCCCCTCGGCCGAGTTCCTGCGGATGATGGGATGTGAGCGGGATGTCCCCGCCCTCTG
  CCGCCTCTTGGAACTGCTGGAGGAGGGCCAGAGGCTGCCGGCGCCTCCTGCCTGCCCTGC
  TGAGGTTCACGAGCTCATGAAGCTGTGCTGGGCCCCTAGCCCACAGGACCGGCCATCATT
  CAGCGCCCTGGGCCCCCAGCTGGACATGCTGTGGAGCGGAAGCCGGGGGTGTGAGACTCA
  TGCCTTCACTGCTCACCCAGAGGGCAAACACCACTCCCTGTCCTTTTCATAGCTCCTGCC
  CGCAGACCTCTGGATTAGGTCTCTGTTGACTGGCTGTGTGACCTTAGGCCCGGAGCTGCC
  CCTCTCTGGGCCTCAGAGGCCTTATGAGGGTCCTCTACTTCAGGAACACCCCCACGACAT
  TGCATTTGGGGGGGCTCCCGTGGCCTGTAGAATAGCCTGTGGCCTTTGCAATTTGTTAAG
  GTTCAAGACAGATGGGCATATGTGTCAGTGGGGCTCTCTGAGTCCTGGCCCAAAGAAGCA
  AGGAACCAAATTTAAGACTCTCGCATCTTCCCAACCCCTTAAGCCCTGGCCCCCTGAGTT
  TCCTTTTCTGTCTCTCTCTTTTTATTTTTTTTATTTTTATTTTTATTTTTGAGACAGAGC
  CTCGCTCTGTTACCCAGGGTGGAGTGCAGTGGTGCGATCTCGGCTCAGTGCAACCTCTGC
  TTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGTG
  CACCACCACACCCGGCTAATTTTTTTTATTTTTAATAGAGATGAGGTTTCACCATGATGG
  CCAGGCTGATCTCGAACTCCTAACCTCAAGTGATCCTCCCACCTCAGCCTCCCAAAGTGT
  TGGAATAATAGGCATGAGCCACTGCACCCAGGCTTTTTTTTTTTTAAATTTATTATTATT
  ATTTTTAAGAGACAGGATCTTGCTACGTTGCCCAGGCTGGTCTTGAACTCCTGGGCTACA
  GTGATCCTCCTGCCTTATCCTCCTAAATAGCTGGGACTACAGCACCTAGTTTTGAGTTTC
  CTGTCTTATTTCCAATGGGGACATTCATGTAGCTTTTTTTTTTTTTTTTTTTTTGAGACG
  GAGTCTCGCTCTGTCGCCCAGGCTGGAGTACAGTGGCGCAATCTAGGCTCACTGCAAGCT
  CCGCCTCCTGGGTTCACACCATTCTCTCGCCTCAGCCTCCCAAGTAGCTGGGACTACAGG
  CGCCCGCCACCACACCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCTTG
  TTAGCCAGGATGGTTTCCATCTCCTGACCTCGTGATCTGCCCGTCTCGGCCTCCCAAAGT
  GCTGGGATTACAGGCATGAGCCACTGCGCCCGGCCCTCATGTAGCTTTAAATGTATGATC
  TGACTTCTGCTCCCCGATCTCTGTTTCTCTGGAGGAAGCCAAGGACAAGAGCAGTTGCTG
  TGGCTGGGACTCTGCCTTTTAGGGGAGCCCGTGTATCTCTTTGGGATCCTGAAAGGGGGC
  AGGAAAGGCTGGGGTCCCAGTCCACCCTAATGGTATCTGAGTGTCCTAGGGCTTCAGTTT
  TCCCACCTGTCCAATGGGACCCTTTCTGTCCTCACCCTACAAGGGGCACAAAGGGATGAC
  ACCAAACCTGGCAGGAACTTTTCACGCAATCAAGGGAAGGAAAGGCATTCCTGGCAGAGG
  GAACAGCATGCCAAGCGTGAGAAGGCTCAGAGTAAGGAGGTTAAGAGCCCAAGTATTGGA
  GCCTACAGTTTTGCCCCTTCCATGCAGTGTGACAGTGGGCAAGTTCCTTTCCCTCTCTGG
  GTCTCAGTTCTGTCCCCTGCAAAATGGTCAGAGCTTACCCCTTGGCTGTGCAGGGTCAAC
  TTTCTGACTGGTGAGAGGGATTCTCATGCAGGTTAAGCTTCTGCTGCTCCTCCTCACCTG
  CAAAGCTTTTCTGCCACTTTTGCCTCCTTGGAAAACTCTTATCCATCTCTCAAAACTCCA
  GCTACCACATCCTTGCAGCCTTCCCTCATATACCCCCACTACTACTGTAGCCCTGTCCTT
  CCCTCCAGCCCCACTCTGGCCCTGGGGCTGGGGAAGTGTCTGTGTCCAGCTGTCTCCCCT
  GACCTCAGGGTTCCTTGGGGGCTGGGCTGAGGCCTCAGTACAGAGGGGGCTCTGGAAATG
  TTTGTTGACTGAATAAAGGAATTCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000215 unedited
  CGGCACACGGACGCCAACTAACAAGGGGGCGGCCGCGAACCCCGGGAACTCGGCCCCGCC
  CGCCCTCCGAAGCCAGGGCCCCCGCCCGCCAGGACCGGGAGCCCCTCCTCTCCGTGCCCC
  GGCAAGCCGCACTCACGGCACCCCCAAGCGAAGAGACGCCCCCGATCCCTCAGCGTTCAT
  GCAGCCTCTCGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCGGGGCCCCG
  GGCCCCCCCAGCGCCTATCTTTCTCCTTTGGGGACCACTTGGCTGAGGACCTGTGCGTGC
  AGGCTGCCAAGGCCAGCGGCATCCTGCCTGTGTACCACTCCCTCTTTGCTCTGGCCACGG
  AGGACCTGTCCTGCTGGTTCCCCCCGAGCCACATCTTCTCCGTGGAGGATGCCAGCACCC
  AAGTCCTGCTGTACAGGATTCGCTTTTACTTCCCCAATTGGTTTGGGCTGGAGAAGTGCC
  ACCGCTTCGGGCTACGCAAGGATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGC
  ACCTCTTTGCCCAGCACCGCAGTGACCTGGTGAGTGGGCGCCTCCCCGTGGGCCTCAGTC
  TCAAGGAGCAGGGTGAGTGTCTCAGCCTGGCCGTGTTGGACCTGGCCCGGATGGCGCGAG
  AGCAGGCCCAGCGGCCGGGAGAGCTGCTGAAGACTGTCAGCTACAAGGCCTGCCTACCCC
  CAAGCCTGCGCGACCTGATCCAGGGCCTGAGCTTCGTGACGCGGAGGCGTATTCGGAGGA
  CGGTGCGCAGAGCCCTGCGCCGCGTGGCCGCTGCCAGGCAGACCGCACTCGCTCATGACA
  GTACATCATGACCTGAGCGCTGATCCAGCCGGGCCGCCGAGACCTTTCCACGTGGCCTCC
  CT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000215 unedited
  NNNCCGTGGCGAGACNAGNCACACGTNCAGGTACGCTGNACTTCGCCTAGCTAAGCTGCT
  GCCGCTTGACAAAGACTACTACGTGGTCCGCGAGCCAGGCCAGAGCCCCATTTTCTGGTA
  TGCCCCCGAATCCCTCTCGGACAACATCTTCTCTCGCCAGTCAGACGTCTGGAGCTTCGG
  GGTCGTCCTGTACGAGCTCTTCACCTACTGCGACAAAAGCTGCAGCCCCTCGGCCGAGTT
  CCTGCGGATGATGGGATGTGAGCGGGATGTCCCCGCCCTCTGCCGCCTCTTGGAACTGCT
  GGAGGAGGGCCAGAGGCTGCCGGCGCCTCCTGCCTGCCCTGCTGAGGTTCACGAGCTCAT
  GAAGCTGTGCTGGGCCCCTAGCCCACAGGACCGGCCATCATTCAGCGCCCTGGGCCCCCA
  GCTGGACATGCTGTGGAGCGGAAGCCGGGGGTGTGAGACTCATGCCTTCACTGCTCACCC
  AGAGGGCAAACACCACTCCCTGTCCTTTTCATAGCTCCTGCCCGCAGACCTCTGGATTAG
  GTCTCTGTTGACTGGCTGTGTGACCTTAGGCCCGGAGCTGCCCCTCTCTGGGCCTCAGAG
  GCCTTATGAGGGTCCTCTACTTCAGGAACACCCCCACGACATTGCATTTGGGGGGGCTCC
  CGTGGCCTGTAGAATAGCCTGTGGCCTTTGCAATTTGTTAAGGTTCAAGACAGATGGGCA
  TATGTGTCAGTGGGGCTCTCTGAGTCCTGGCCCANAAAGCAGGAACCAAATTAAGACTCT
  CGCATCTTCCCAACCCCTTAAGCCCTGGCCCCCTGAGTTTCCTTTTCAGGCNCTCTCTTT
  TTATTTTTTTATTTTTATTTTTATTTTTGAGACGAGCCTCGCTCTGTTACCCAGGGTGGA
  G
  
• Restriction Sites: Please inquire
• ACCN: NM_000215
• Insert Size: 5500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000215.2, NP_000206.2
• RefSeq Size: 4025 bp
• RefSeq ORF: 3375 bp
• Locus ID: 3718
• UniProt ID: P52333
• Cytogenetics: 19p13.11
• Domains: B41, pkinase, SH2, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway, Primary immunodeficiency
• Gene Summary: The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]