NRG1 (NM_004495) Human Untagged Clone

CAT#: SC310625

NRG1 (untagged)-Human neuregulin 1 (NRG1), transcript variant HRG-gamma

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRG1
• Synonyms: ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1-IT2; SMDF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004495 edited
  GGGCTCGCGCGGAGGCCAGGAGCTGAGCGGCGGCGGCTGCCGGACGATGGGAGCGTGAGC
  AGGACGGTGATAACCTCTCCCCGATCGGGTTGCGAGGGCGCCGGGCAGAGGCCAGGACGC
  GAGCCGCCAGCGGCGGGACCCATCGACGACTTCCCGGGGCGACAGGAGCAGCCCCGAGAG
  CCAGGGCGAGCGCCCGTTCCAGGTGGCCGGACCGCCCGCCGCGTCCGCGCCGCGCTCCCT
  GCAGGCAACGGGAGACGCCCCCGCGCAGCGCGAGCGCCTCAGCGCGGCCGCTCGCTCTCC
  CCATCGAGGGACAAACTTTTCCCAAACCCGATCCGAGCCCTTGGACCAAACTCGCCTGCG
  CCGAGAGCCGTCCGCGTAGAGCGCTCCGTCTCCGGCGAGATGTCCGAGCGCAAAGAAGGC
  AGAGGCAAAGGGAAGGGCAAGAAGAAGGAGCGAGGCTCCGGCAAGAAGCCGGAGTCCGCG
  GCGGGCAGCCAGAGCCCAGCCTTGCCTCCCCGATTGAAAGAGATGAAAAGCCAGGAATCG
  GCTGCAGGTTCCAAACTAGTCCTTCGGTGTGAAACCAGTTCTGAATACTCCTCTCTCAGA
  TTCAAGTGGTTCAAGAATGGGAATGAATTGAATCGAAAAAACAAACCACAAAATATCAAG
  ATACAAAAAAAGCCAGGGAAGTCAGAACTTCGCATTAACAAAGCATCACTGGCTGATTCT
  GGAGAGTATATGTGCAAAGTGATCAGCAAATTAGGAAATGACAGTGCCTCTGCCAATATC
  ACCATCGTGGAATCAAACGAGATCATCACTGGTATGCCAGCCTCAACTGAAGGAGCATAT
  GTGTCTTCAGAGTCTCCCATTAGAATATCAGTATCCACAGAAGGAGCAAATACTTCTTCA
  TCTACATCTACATCCACCACTGGGACAAGCCATCTTGTAAAATGTGCGGAGAAGGAGAAA
  ACTTTCTGTGTGAATGGAGGGGAGTGCTTCATGGTGAAAGACCTTTCAAACCCCTCGAGA
  TACTTGTGCAAGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004495
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004495.1, NP_004486.1
• RefSeq Size: 1651 bp
• RefSeq ORF: 636 bp
• Locus ID: 3084
• Cytogenetics: 8p12
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: ErbB signaling pathway
• Gene Summary: The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (HRG-gamma), which uses the type I promoter, lacks multiple 3' exons but contains an alternate 3' terminal exon that results in an early stop codon, compared to variant HRG-beta1. The resulting isoform (HRG-gamma) is shorter at the C-terminus, compared to isoform HRG-beta1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.