QPRT (NM_014298) Human Untagged Clone

CAT#: SC310560

QPRT (untagged)-Human quinolinate phosphoribosyltransferase (QPRT)


  "NM_014298" in other vectors (7)

Reconstitution Protocol

USD 480.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
QPRT mouse monoclonal antibody, clone OTI1C10 (formerly 1C10)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "QPRT"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol QPRT
Synonyms HEL-S-90n; QPRTase
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310560 representing NM_014298.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACGCTGAAGGCCTGGCGCTGCTGCTGCCGCCCGTCACCCTGGCAGCCCTGGTGGACAGCTGGCTC
CGAGAGGACTGCCCAGGGCTCAACTACGCAGCCTTGGTCAGCGGGGCAGGCCCCTCGCAGGCGGCGCTG
TGGGCCAAATCCCCTGGGGTACTGGCAGGGCAGCCTTTCTTCGATGCCATATTTACCCAACTCAACTGC
CAAGTCTCCTGGTTCCTCCCCGAGGGATCGAAGCTGGTGCCGGTGGCCAGAGTGGCCGAGGTCCGGGGC
CCTGCCCACTGCCTGCTGCTGGGGGAACGGGTGGCCCTCAACACGCTGGCCCGCTGCAGTGGCATTGCC
AGTGCTGCCGCCGCTGCAGTGGAGGCCGCCAGGGGGGCCGGCTGGACTGGGCACGTGGCAGGCACGAGG
AAGACCACGCCAGGCTTCCGGCTGGTGGAGAAGTATGGGCTCCTGGTGGGCGGGGCCGCCTCGCACCGC
TACGACCTGGGAGGGCTGGTGATGGTGAAGGATAACCATGTGGTGGCCGCCGGTGGCGTGGAGAAGGCG
GTGCGGGCGGCCAGACAGGCGGCTGACTTCGCTCTGAAGGTGGAAGTGGAATGCAGCAGCCTGCAGGAG
GCCGTGCAGGCAGCTGAGGCTGGTGCCGACCTTGTCCTGCTGGACAACTTCAAGCCAGAGGAGCTGCAC
CCCACGGCCACCGTGCTGAAGGCCCAGTTCCCGAGTGTGGCTGTGGAAGCCAGTGGGGGCATCACCCTG
GACAACCTCCCCCAGTTCTGCGGGCCGCACATAGACGTCATCTCCATGGGGATGCTGACCCAGGCGGCC
CCAGCCCTTGATTTCTCCCTCAAGCTGTTTGCCAAAGAGGTGGCTCCAGTGCCCAAAATCCACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_014298
Insert Size 894 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014298.4
RefSeq Size 2385 bp
RefSeq ORF 894 bp
Locus ID 23475
UniProt ID Q15274
Cytogenetics 16p11.2
Domains QRPTase
Protein Pathways Metabolic pathways, Nicotinate and nicotinamide metabolism
MW 30.8 kDa
Gene Summary This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.