alpha 1 Glycine Receptor (GLRA1) (NM_000171) Human Untagged Clone

CAT#: SC310373

GLRA1 (untagged)-Human glycine receptor, alpha 1 (GLRA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: alpha 1 Glycine Receptor
• Synonyms: HKPX1; STHE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000171 edited
  GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTCCTTGCAGGGAGCTGGCCGTTT
  AAACAGAAAAACAGGGGTTTAAAAAAAAAAAAAAAAAAGGAAATATACCCACCCCCAAAC
  GTGCCTCCCCAGCGCCGCAGGGAGCCAACAGACACGCTGGAGTTTAACAAACAGCAATAC
  TCTTCGCGCTCCTGAAAAGCAGGTCTGGACGCTCTCCGTGGTGCTGAAACGCCTCGTAGC
  CGCCGCTGTCCGTGGTATCTACGACCCCCTCGCTCCAATTTCCCCTGGGGCTCTCCCTCC
  GCGCCCCTGTTCCCCGCCTCCCTTTAACATCTGGATTATTTTTTGCAATAGCGCTTTCTG
  GTTTTGTAAGTGCCAATTTGAAACATTTTTGCCCCCATAACTCGTGGACTACAAAGCACA
  AGGACCTGAAAAATGTACAGCTTCAATACTCTTCGACTCTACCTTTGGGAGACCATTGTA
  TTCTTCAGCCTTGCTGCTTCTAAGGAGGCTGAAGCTGCTCGCTCCGCACCCAAGCCTATG
  TCACCCTCGGATTTCCTGGATAAGCTAATGGGGAGAACCTCCGGATATGATGCCAGGATC
  AGGCCCAATTTTAAAGGTCCCCCAGTGAACGTGAGCTGCAACATTTTCATCAACAGCTTT
  GGTTCCATTGCTGAGACAACCATGGACTATAGGGTCAACATCTTCCTGCGGCAGCAATGG
  AACGACCCCCGCCTGGCCTATAATGAATACCCTGACGACTCTCTGGACCTGGACCCATCC
  ATGCTGGACTCCATCTGGAAACCTGACCTGTTCTTTGCCAACGAGAAGGGGGCCCACTTC
  CATGAGATCACCACAGACAACAAATTGCTAAGGATCTCCCGGAATGGGAATGTCCTCTAC
  AGCATCAGAATCACCCTGACACTGGCCTGCCCCATGGACTTGAAGAATTTCCCCATGGAT
  GTCCAGACATGTATCATGCAACTGGAAAGCTTTGGATATACGATGAATGACCTCATCTTT
  GAGTGGCAGGAACAGGGAGCCGTGCAGGTAGCAGATGGACTAACTCTGCCCCAGTTTATC
  TTGAAGGAAGAGAAGGACTTGAGATACTGCACCAAGCACTACAACACAGGTAAATTCACC
  TGCATTGAGGCCCGGTTCCACCTGGAGCGGCAGATGGGTTACTACCTGATTCAGATGTAT
  ATTCCCAGCCTGCTCATTGTCATCCTCTCATGGATCTCCTTCTGGATCAACATGGATGCT
  GCACCTGCTCGTGTGGGCCTAGGCATCACCACTGTGCTCACCATGACCACCCAGAGCTCC
  GGCTCTCGAGCATCTCTGCCCAAGGTGTCCTATGTGAAAGCCATTGACATTTGGATGGCA
  GTTTGCCTGCTCTTTGTGTTCTCAGCCCTATTAGAATATGCTGCCGTTAACTTTGTGTCT
  CGGCAACATAAGGAGCTGCTCCGATTCAGGAGGAAGCGGAGACATCACAAGGAGGATGAA
  GCTGGAGAAGGCCGCTTTAACTTCTCTGCCTATGGGATGGGCCCAGCCTGTCTACAGGCC
  AAGGATGGCATCTCAGTCAAGGGCGCCAACAACAGTAACACCACCAACCCCCCTCCTGCA
  CCATCTAAGTCCCCAGAGGAGATGCGAAAACTCTTCATCCAGAGGGCCAAGAAGATCGAC
  AAAATATCCCGCATTGGCTTCCCCATGGCCTTCCTCATTTTCAACATGTTCTACTGGATC
  ATCTACAAGATTGTCCGTAGAGAGGACGTCCACAACCAGTGAAGGGTCTGAAAGGTTGGG
  GGAGGCTGGGAGAGGGGAACGTGGGAATAGCACAGGAATAAGGGCGAATTCAGATCTGGT
  ACCGATATCAAGCTTGTCGACTCTAGATTGCGGCCGC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000171 unedited
  TCCCGTCANAAATTGTATACGACTCATATAGGCGGCCGCAATCTAGAGTCGAGTTTTTTT
  TTTTTTTTTCCTTGCAGGGAGCTGGCCGTTTAAACAGAAAAACAGGGGTTTAAAAAAAAA
  AAAAAAAAAGGAAATATACCCACCCCCAAACGTGCCTCCCCAGCGCCGCAGGGAGCCAAC
  AGACACGCTGGAGTTTAACAAACAGCAATACTCTTCGCGCTCCTGAAAAGCAGGTCTGGA
  CGCTCTCCGTGGTGCTGAAACGCCTCGTAGCCGCCGCTGTCCGTGGTATCTACGACCCCC
  TCGCTCCAATTTCCCCTGGGGCTCTCCCTCCGCGCCCCTGTTCCCCGCCTCCCTTTAACA
  TCTGGATTATTTTTTGCAATAGCGCTTTCTGGTTTTGTAAGTGCCAATTTGAAACATTTT
  TGCCCCCATAACTCGTGGACTACAAAGCACAAGGACCTGAAAAATGTACAGCTTCAATAC
  TCTTCGACTCTACCTTTGGGAGACCATTGTATTCTTCAGCCTTGCTGCTTCTAAGGAGGC
  TGAAGCTGCTCGCTCCGCACCCAAGCCTATGTCACCCTCGGATTTCCTGGATAAGCTAAT
  GGGGAGAACCTCCGGATATGATGCCAGGATCAGGCCCAATTTTAAAGGTCCCCCAGTGAA
  CGTGAGCTGCAACATTTTCATCAACAGCTTTGGTTCCATTGCTGAGCAACCATGGACTAT
  AGGGTCAACATCTTCCTGCGGCAGCAATGGAACGACCCCCGCCTGGCCTATAATGAATAC
  CCTGACGACTCTCTGGACCTGGACCCATCCATGCTGGACTCCATCTGGAAACCTGACCTG
  TTCTTTGCCAACGAGAAGGGGGCCCACTTCCATGAGATCACCACAGACACAAATGCTAAG
  GATCTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000171 unedited
  CCCTGCTATGGCGAGGCAACTTCCAGGGCAGGNATAGCACTGGGGGAGGGTCACAGGGCT
  GCCACCCGGGCACTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAG
  CTTGATATCGGTACCAGATCTGAATTCGCCCTTATTCCTGTGCTATTCCCACGTTCCCCT
  CTCCCAGCCTCCCCCAACCTTTCAGACCCTTCACTGGTTGTGGACGTCCTCTCTACGGAC
  AATCTTGTAGATGATCCAGTAGAACATGTTGAAAATGAGGAAGGCCATGGGGAAGCCAAT
  GCGGGATATTTTGTCGATCTTCTTGGCCCTCTGGATGAAGAGTTTTCGCATCTCCTCTGG
  GGACTTANATGGTGCAGGAGGGGGGTTGGTGGTGTTACTGTTGTTGGCGCCCTTGACTGA
  GATGCCATCCTTGGCCTGTAGACAGGCTGGGCCCATCCCATAGGCAGAGAAGTTAAAGCG
  GCCTTCTCCAGCTTCATCCTCCTTGTGATGTCTCCGCTTCCTCCTGAATCGGAGCAGCTC
  CTTATGTTGCCGAGACACAAAGTTAACGGCAGCATATTCTAATAGGGCTGAGAACACAAA
  GAGCAGGCAAACTGCCATCCAAATGTCAATGGCTTTCACATAGGACACCTTGGGCAGAGA
  TGCTCGAGAGCCGGAGCTCTGGGTGGTCATGGTGAGCACAGTGGTGATGCCTAGGCCCAC
  ACGAGCAGGTGCAGCATCCATGTTGATCCCAGAAGAGATCCATGAGAGGATGACAATGAG
  CAGGCTGGGAATATACATCTGAATCAGGTAGTAACCCATCTGCCGCTCCAGGTGGAACCG
  GGCCCTCATGCAGGTGAATTTACCTGTGTTGTANTGCTTGGTGCAGTATCTCAAGTCCTT
  CTTCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_000171
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 4 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 4 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000171.1, NP_000162.1
• RefSeq Size: 1715 bp
• RefSeq ORF: 1350 bp
• Locus ID: 2741
• UniProt ID: P23415
• Cytogenetics: 5q33.1
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of the last exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.