MAP2 (NM_002374) Human Untagged Clone

CAT#: SC309886

MAP2 (untagged)-Human microtubule-associated protein 2 (MAP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP2
• Synonyms: MAP-2; MAP2A; MAP2B; MAP2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309886 representing NM_002374.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAGATGAACGGAAAGATGAAGCAAAGGCACCTCACTGGACCTCAGCACCGCTAACAGAGGCATCT
  GCACACTCACATCCACCTGAGATTAAGGATCAAGGCGGAGCAGGGGAAGGACTTGTCCGAAGCGCCAAT
  GGATTCCCATACAGGGAGGATGAAGAGGGTGCCTTTGGAGAGCATGGGTCACAGGGCACCTATTCAAAT
  ACCAAAGAGAATGGGATCAACGGAGAGCTGACCTCAGCTGACAGAGAAACAGCAGAGGAGGTGTCTGCA
  AGGATAGTTCAAGTAGTCACTGCTGAGGCTGTAGCAGTCCTGAAAGGTGAACAAGAGAAAGAAGCTCAA
  CATAAAGACCAGACTGCAGCTCTGCCTTTAGCAGCTGAAGAAACAGCTAATCTGCCTCCTTCTCCACCC
  CCATCACCTGCCTCAGAACAGACTGTCACAGTGGAGGAAGATTTACTTACAGCCTCGAAGATGGAGTTC
  CACGATCAACAGGAATTGACTCCCTCTACAGCTGAGCCTTCAGACCAGAAGGAAAAGGAGTCAGAGAAG
  CAAAGTAAGCCTGGTGAAGACCTTAAACATGCTGCCTTAGTTTCTCAGCCAGAGACAACTAAAACTTAC
  CCTGATAAAAAGGACATGCAAGGCACGGAAGAAGAAAAAGCACCCCTAGCTTTGTTTGGGCACACTCTT
  GTTGCCAGCCTGGAAGACATGAAACAGAAGACAGAACCAAGCCTTGTAGTACCTGGCATTGACCTCCCT
  AAAGAGCCTCCAACTCCAAAAGAACAAAAGGACTGGTTCATCGAAATGCCAACGGAAGCAAAAAAGGAT
  GAGTGGGGTTTAGTTGCCCCCATATCTCCTGGCCCTCTGACTCCCATGAGGGAAAAAGATGTATTTGAT
  GATATCCCAAAATGGGAAGGGAAACAGTTTGATTCTCCCATGCCAAGTCCCTTTCAAGGGGGAAGCTTC
  ACTCTTCCTTTAGATGTCATGAAGAATGAAATAGTTACAGAAACATCGCCCTTTGCCCCTGCCTTTTTA
  CAGCCAGATGACAAAAAATCTCTGCAACAAACCAGTGGCCCAGCTACTGCCAAAGATAGTTTTAAAATT
  GAAGAGCCCCATGAGGCTAAACCTGACAAAATGGCAGAAGCACCACCCTCAGAGGCAATGACCTTACCC
  AAAGATGCTCACATTCCAGTTGTAGAAGAACATGTTATGGGGAAAGTTTTAGAGGAAGAAAAGGAGGCC
  ATAAATCAAGAGACTGTGCAGCAAAGGGATACTTTCACCCCCAGTGGACAGGAACCTATACTTACTGAA
  AAGGAAACTGAGCTGAAGCTTGAAGAAAAAACCACCATTTCTGACAAAGAAGCTGTGCCAAAAGAGAGT
  AAACCCCCAAAACCTGCAGATGAAGAAATAGGCATAATTCAGACCTCCACAGAGCACACTTTCTCAGAA
  CAGAAAGACCAAGAGCCTACCACAGATATGTTGAAACAGGACTCGTTCCCTGTAAGTTTGGAGCAAGCA
  GTTACAGATTCAGCCATGACCTCTAAAACACTGGAGAAAGCCATGACCGAACCATCTGCATTAATTGAA
  AAGAGCTCAATTCAGGAACTTTTTGAAATGAGAGTTGATGACAAAGATAAGATTGAAGGAGTTGGAGCT
  GCAACATCAGCTGAGCTTGATATGCCATTTTATGAAGATAAATCAGGAATGTCCAAGTACTTTGAAACA
  TCTGCCTTGAAAGAAGAAGCAACAAAAAGCATTGAGCCAGGCAGTGATTACTATGAACTGAGTGACACT
  AGAGAAAGTGTCCATGAGTCTATTGATACCATGTCTCCCATGCATAAAAATGGTGACAAGGAGTTTCAA
  ACAGGAAAAGAATCCCAGCCCAGTCCTCCAGCACAAGAAGCAGGGTACAGCACTCTCGCACAGAGTTAT
  CCATCAGATTTACCTGAAGAACCCAGTTCTCCTCAAGAAAGAATGTTCACTATTGATCCAAAAGTGTAT
  GGAGAGAAAAGGGACCTCCACAGTAAGAATAAGGATGATTTGACCCTTAGCAGGAGTTTAGGACTTGGT
  GGTAGGTCTGCAATAGAACAAAGAAGCATGTCAATCAATTTGCCGATGTCTTGCCTAGATTCCATAGCC
  CTTGGATTTAACTTTGGTCGGGGACATGATCTTTCTCCTCTGGCTTCCGATATTCTAACCAACACTAGT
  GGAAGTATGGATGAAGGGGATGATTACCTTCCAGCCACCACACCTGCACTGGAGAAAGCCCCTTGCTTC
  CCTGTAGAAAGCAAAGAGGAAGAACAGATAGAGAAAGTAAAAGCTACTGGAGAAGAAAGTACTCAAGCG
  GAGATATCATGTGAGTCTCCTTTCCTAGCCAAAGATTTTTACAAAAATGGTACTGTCATGGCACCTGAC
  CTTCCTGAAATGCTAGATCTGGCAGGCACAAGGTCAAGATTGGCTTCTGTGAGTGCAGATGCTGAGGTT
  GCCAGGAGGAAATCAGTCCCATCAGAGACTGTGGTTGAGGATAGTCGTACTGGCTTGCCCCCGGTAACT
  GATGAAAACCATGTCATTGTAAAAACGGACAGTCAGCTCGAAGACCTGGGCTACTGTGTGTTCAATAAG
  TACACAGTCCCATTGCCATCACCTGTTCAAGACAGTGAGAATTTATCAGGGGAGAGTGGTACCTTTTAC
  GAAGGCACTGATGATAAAGTTCGAAGAGATTTGGCCACAGACCTTTCACTGATTGAAGTGAAACTGGCA
  GCAGCCGGAAGAGTCAAAGATGAGTTCAGTGTTGACAAAGAAGCATCCGCGCATATCTCTGGTGACAAA
  TCAGGACTGAGTAAGGAGTTTGACCAAGAGAAGAAAGCTAATGATAGGTTGGATACTGTACTAGAAAAG
  AGTGAAGAACATGCTGATTCAAAAGAACATGCCAAGAAAACTGAAGAGGCTGGTGATGAAATAGAAACA
  TTCGGATTAGGAGTAACCTATGAGCAAGCTTTGGCCAAAGATTTGTCAATACCAACAGATGCATCCTCT
  GAGAAAGCAGAGAAGGGTCTTAGTTCAGTGCCAGAGATAGCTGAGGTAGAACCATCCAAAAAGGTGGAA
  CAAGGTCTGGATTTTGCTGTCCAGGGTCAACTAGATGTTAAAATTAGTGACTTTGGACAGATGGCTTCA
  GGGCTAAACATAGATGATAGAAGGGCAACAGAGCTAAAACTTGAGGCTACACAGGACATGACCCCCTCA
  TCCAAAGCACCGCAGGAGGCAGATGCATTTATGGGTGTTGAGTCTGGCCACATGAAAGAAGGCACTAAA
  GTTAGTGAGACAGAAGTCAAAGAGAAGGTGGCCAAGCCTGACTTGGTGCACCAGGAGGCTGTAGACAAG
  GAGGAGTCCTATGAATCTAGTGGTGAGCATGAAAGTCTCACCATGGAGTCCTTGAAAGCTGATGAGGGC
  AAGAAGGAAACATCTCCAGAATCATCTCTAATTCAAGATGAGATTGCCGTCAAATTGTCAGTGGAAATA
  CCTTGCCCACCTGCTGTTTCAGAGGCTGATTTAGCCACAGATGAGAGAGCTGATGTCCAGATGGAATTT
  ATTCAGGGGCCAAAAGAAGAAAGCAAAGAGACCCCAGATATATCCATCACGCCTTCTGATGTTGCAGAG
  CCATTGCATGAAACGATCGTATCTGAACCAGCAGAGATTCAGAGTGAGGAAGAAGAGATAGAAGCCCAG
  GGAGAATATGATAAACTGCTCTTCCGCTCAGACACCCTTCAGATAACTGACCTGGGTGTCTCAGGTGCC
  AGGGAGGAATTTGTGGAGACCTGCCCAAGTGAACACAAAGGAGTGATTGAGTCTGTTGTGACCATCGAG
  GATGATTTCATCACTGTAGTGCAAACCACAACTGATGAAGGGGAGTCAGGGTCCCACAGCGTGCGTTTT
  GCAGCCCTAGAGCAGCCTGAGGTGGAAAGGAGACCATCTCCTCATGATGAAGAAGAGTTTGAAGTAGAA
  GAGGCAGCTGAAGCCCAGGCAGAACCCAAAGATGGTTCCCCAGAGGCTCCAGCTTCCCCTGAGAGAGAA
  GAGGTTGCACTTTCTGAATATAAGACAGAAACCTATGACGATTACAAAGATGAGACCACCATTGACGAC
  TCCATCATGGACGCTGACAGCCTCTGGGTGGACACTCAAGATGATGATAGGAGCATCATGACAGAACAG
  TTAGAAACTATTCCTAAAGAGGAGAAAGCTGAAAAGGAAGCTCGGAGATCATCTCTTGAGAAACATAGA
  AAAGAAAAGCCTTTTAAAACCGGGAGAGGCAGAATTTCCACTCCTGAAAGAAAAGTAGCTAAAAAGGAA
  CCTAGCACAGTCTCCAGAGATGAAGTGAGAAGGAAAAAAGCAGTTTATAAGAAGGCTGAACTTGCTAAA
  AAAACAGAAGTTCAGGCCCACTCTCCCTCCAGGAAATTCATTTTAAAACCTGCTATCAAATATACTAGA
  CCAACTCATCTCTCCTGTGTTAAGCGGAAAACCACAGCAGCAGGTGGGGAATCAGCTCTGGCTCCCAGT
  GTATTTAAACAGGCAAAGGACAAAGTCTCTGACGGAGTAACCAAGAGCCCAGAAAAGCGCTCTTCTCTC
  CCAAGACCTTCCTCCATTCTCCCTCCTCGGCGAGGTGTGTCAGGAGACAGAGATGAGAATTCCTTCTCT
  CTCAACAGTTCTATCTCTTCTTCAGCACGGCGGACCACCAGGTCAGAGCCAATTCGCAGAGCAGGGAAG
  AGTGGTACCTCAACACCCACTACCCCTGGGTCTACTGCCATCACTCCTGGCACCCCACCAAGTTATTCT
  TCACGCACACCAGGCACTCCTGGAACCCCTAGCTATCCCAGGACCCCTCACACACCAGGAACCCCCAAG
  TCTGCCATCTTGGTGCCGAGTGAGAAGAAGGTCGCCATCATACGTACTCCTCCAAAATCTCCTGCGACT
  CCCAAGCAGCTTCGGCTTATTAACCAACCACTGCCAGACCTGAAGAATGTCAAATCCAAAATCGGATCA
  ACAGACAACATCAAATACCAGCCTAAAGGGGGGCAGGTACAAATTGTTACCAAGAAAATAGACCTAAGC
  CATGTGACATCCAAATGTGGCTCTCTGAAGAACATCCGCCACAGGCCAGGTGGCGGACGTGTGAAAATT
  GAGAGTGTAAAACTAGATTTCAAAGAAAAGGCCCAAGCTAAAGTTGGTTCTCTTGATAATGCTCATCAT
  GTACCTGGAGGTGGTAATGTCAAGATTGACAGCCAAAAGTTGAACTTCAGAGAGCATGCTAAAGCCCGT
  GTGGACCATGGGGCTGAGATCATTACACAGTCCCCAGGCAGATCCAGCGTGGCATCACCCCGACGACTC
  AGCAATGTCTCCTCGTCTGGAAGCATCAACCTGCTCGAATCTCCTCAGCTTGCCACTTTGGCTGAGGAT
  GTCACTGCTGCACTCGCTAAGCAGGGCTTGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002374
• Insert Size: 5484 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002374.3
• RefSeq Size: 9445 bp
• RefSeq ORF: 5484 bp
• Locus ID: 4133
• UniProt ID: P11137
• Cytogenetics: 2q34
• Domains: tubulin-binding
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS
• MW: 199.5 kDa
• Gene Summary: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
  Transcript Variant: This longest variant (1) includes three alternate in-frame exons, compared to variant 2, resulting in the longest isoform (1).