Myoferlin (MYOF) (NM_013451) Human Untagged Clone

CAT#: SC309765

MYOF (untagged)-Human myoferlin (MYOF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Myoferlin
• Synonyms: FER1L3; HAE7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309765 representing NM_013451.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCGAGTGATTGTGGAATCTGCCAGCAATATCCCTAAAACGAAATTTGGCAAGCCGGATCCTATT
  GTTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAATTGAACCCTGTCTGG
  AATGAGATTTTGGAGTTTGACTTGAGGGGTATACCACTGGACTTTTCATCTTCCCTTGGGATTATTGTG
  AAAGATTTTGAGACAATTGGACAAAATAAATTAATTGGCACGGCGACTGTAGCCCTGAAGGACCTGACT
  GGTGACCAGAGCAGATCCCTGCCGTACAAGCTGATCTCCCTGCTAAATGAAAAAGGGCAAGATACTGGG
  GCCACCATTGACTTGGTGATCGGCTATGATCCGCCTTCTGCTCCACATCCAAATGACCTGAGCGGGCCC
  AGCGTGCCAGGCATGGGAGGAGATGGGGAAGAAGATGAAGGTGATGAAGACAGGTTGGACAATGCAGTC
  AGGGGCCCTGGGCCCAAGGGGCCAGTTGGGACGGTGTCGGAAGCTCAGCTTGCTCGGAGGCTCACCAAA
  GTAAAGAACAGCCGGCGGATGCTGTCAAATAAGCCACAGGACTTCCAGATCCGCGTCCGAGTGATTGAG
  GGCCGACAGTTAAGTGGTAACAACATAAGGCCTGTGGTCAAAGTTCACGTCTGTGGCCAGACACACCGA
  ACAAGAATCAAGAGAGGAAACAACCCTTTTTTTGATGAGTTGTTTTTCTACAATGTCAACATGACCCCT
  TCTGAATTGATGGATGAGATCATCAGCATCCGGGTTTATAATTCTCACTCTCTGCGGGCAGATTGTCTG
  ATGGGGGAATTTAAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCATGCTGTCATGAGAAAGTGG
  CTTCTTCTCAATGACCCGGAAGATACCAGTTCAGGTTCTAAAGGTTATATGAAAGTCAGCATGTTTGTC
  CTGGGAACCGGAGATGAGCCTCCTCCTGAGAGACGAGATCGTGATAATGACAGTGATGATGTGGAGAGT
  AATTTGTTACTCCCTGCTGGCATTGCCCTCCGGTGGGTGACCTTCTTGCTGAAAATCTACCGAGCTGAG
  GACATCCCCCAGATGGATGATGCCTTCTCACAGACAGTAAAGGAAATATTTGGAGGCAATGCAGATAAG
  AAAAATCTCGTGGATCCTTTTGTAGAAGTTTCCTTTGCTGGAAAAAAGGTTTGTACAAACATAATTGAG
  AAAAATGCAAACCCAGAGTGGAATCAGGTCGTCAATCTTCAGATCAAGTTTCCTTCAGTGTGTGAAAAA
  ATAAAACTAACAATATATGACTGGGACCGTCTTACTAAAAATGATGTAGTTGGAACAACATATCTACAC
  CTCTCTAAAATTGCTGCCTCTGGTGGGGAAGTGGAAGATTTCTCATCTTCGGGAACTGGGGCTGCATCA
  TATACAGTAAACACAGGAGAAACAGAGGTAGGCTTTGTTCCAACGTTTGGACCTTGTTACCTGAATCTT
  TATGGAAGCCCCAGAGAGTACACGGGATTCCCAGACCCCTATGATGAGCTGAATACTGGAAAGGGGGAA
  GGAGTTGCCTACAGAGGCAGGATCTTGGTTGAATTAGCCACTTTTCTTGAGAAGACACCACCAGATAAA
  AAGCTTGAGCCCATTTCAAATGATGACCTGCTGGTTGTTGAGAAATACCAGCGAAGGCGGAAGTACAGC
  CTGTCTGCCGTGTTTCATTCAGCCACCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAGTCAGC
  ATTGGGAACTATGGCAACAAGTTTGACACCACCTGTAAGCCTTTGGCATCAACAACTCAGTACAGCCGT
  GCTGTATTTGATGGCAACTACTATTATTACTTGCCTTGGGCCCACACCAAGCCAGTTGTTACCCTGACT
  TCATACTGGGAGGATATTAGTCATCGCCTGGATGCGGTGAACACTCTCCTAGCTATGGCAGAACGGCTG
  CAAACAAATATAGAAGCTCTAAAATCAGGGATACAAGGTAAAATTCCTGCAAACCAGCTGGCTGAATTG
  TGGCTGAAGCTGATAGATGAAGTTATAGAAGACACGAGATACACGTTGCCTCTCACAGAAGGAAAAGCC
  AACGTCACAGTTCTCGATACTCAGATCCGAAAGCTGCGGTCCAGGTCTCTCTCCCAAATACATGAGGCG
  GCTGTGAGGATGAGGTCGGAAGCCACAGATGTGAAGTCCACACTGGCAGAAATTGAGGACTGGCTTGAT
  AAATTAATGCAGCTGACTGAAGAGCCACAGAACAGCATGCCTGACATCATCATCTGGATGATCCGGGGA
  GAGAAGAGACTGGCCTATGCACGAATTCCCGCACATCAGGTCTTGTACTCCACCAGTGGTGAGAATGCA
  TCTGGAAAATACTGTGGGAAAACCCAAACCATCTTTCTGAAGTATCCACAGGAGAAAAACAACGGGCCA
  AAGGTGCCTGTGGAGTTGCGAGTGAACATCTGGCTAGGCTTAAGTGCTGTGGAGAAGAAGTTTAACAGC
  TTCGCAGAAGGAACTTTCACCGTCTTTGCTGAAATGTATGAAAATCAAGCTCTCATGTTTGGAAAATGG
  GGTACTTCTGGATTAGTAGGACGTCATAAGTTTTCTGATGTCACAGGAAAAATAAAACTCAAGAGGGAA
  TTTTTTCTGCCTCCAAAAGGCTGGGAATGGGAAGGAGAGTGGATAGTTGATCCTGAAAGAAGCTTGCTG
  ACTGAGGCAGATGCAGGTCACACGGAGTTCACTGATGAAGTCTATCAGAACGAGAGCCGCTACCCCGGG
  GGCGACTGGAAGCCGGCCGAGGACACCTACACGGATGCGAACGGCGATAAAGCAGCATCACCCAGCGAG
  TTGACTTGTCCTCCAGGTTGGGAATGGGAAGATGATGCATGGTCTTATGACATAAATCGAGCGGTGGAT
  GAGAAAGGCTGGGAATATGGAATCACCATTCCTCCTGATCATAAGCCCAAATCCTGGGTTGCAGCAGAG
  AAAATGTACCACACTCATAGACGGCGAAGGCTGGTCCGAAAACGCAAGAAAGATTTAACACAGACTGCT
  TCAAGCACCGCAAGGGCCATGGAGGAATTGCAAGACCAAGAGGGCTGGGAATATGCTTCTCTAATTGGC
  TGGAAATTTCACTGGAAACAACGTAGTTCAGATACCTTCCGCCGCAGACGCTGGAGGAGAAAAATGGCT
  CCTTCAGAAACACATGGTGCAGCTGCCATCTTTAAACTTGAAGGTGCCCTTGGGGCAGACACTACCGAA
  GATGGGGATGAGAAGAGCCTGGAGAAACAGAAGCACAGTGCCACCACTGTGTTCGGAGCAAACACCCCC
  ATTGTTTCCTGCAATTTTGACAGAGTCTACATCTACCATCTGCGCTGCTATGTCTATCAAGCCAGAAAC
  CTCTTGGCTTTAGATAAGGATAGCTTTTCAGATCCATATGCTCATATCTGTTTCCTCCATCGGAGCAAA
  ACCACTGAGATCATCCATTCAACCCTGAATCCCACGTGGGACCAAACAATTATATTCGATGAAGTTGAA
  ATCTATGGGGAACCCCAAACAGTTCTACAGAATCCACCCAAAGTTATCATGGAACTTTTTGACAATGAC
  CAAGTGGGCAAAGATGAATTTTTAGGACGAAGCATTTTCTCTCCTGTGGTGAAACTGAACTCAGAAATG
  GACATCACACCCAAACTTCTCTGGCACCCAGTAATGAATGGAGACAAAGCCTGCGGGGATGTTCTTGTA
  ACTGCAGAGCTGATTCTGAGGGGCAAGGATGGCTCCAACCTTCCCATTCTTCCCCCTCAAAGGGCGCCA
  AATCTATACATGGTCCCCCAGGGGATCAGGCCTGTGGTCCAGCTCACTGCCATTGAGATTCTAGCTTGG
  GGCTTAAGAAATATGAAAAACTTCCAGATGGCTTCTATCACATCCCCCAGTCTTGTTGTGGAGTGTGGA
  GGAGAAAGGGTGGAATCGGTGGTGATCAAAAACCTTAAGAAGACACCCAACTTTCCAAGTTCTGTTCTC
  TTCATGAAAGTGTTCTTGCCCAAGGAGGAATTGTACATGCCCCCACTGGTGATCAAGGTCATCGACCAC
  AGGCAGTTTGGGCGGAAGCCTGTCGTCGGCCAGTGCACCATCGAGCGCCTGGACCGCTTTCGCTGTGAC
  CCTTATGCAGGGAAAGAGGACATCGTCCCACAGCTCAAAGCCTCCCTTCTGTCTGCCCCACCATGCCGG
  GACATCGTTATCGAAATGGAAGACACCAAACCATTACTGGCTTCTAAGCTGACAGAAAAGGAGGAAGAA
  ATCGTGGACTGGTGGAGTAAATTTTATGCTTCCTCAGGGGAACATGAAAAATGCGGACAGTATATTCAG
  AAAGGCTATTCCAAGCTCAAGATATATAATTGTGAACTAGAAAATGTAGCAGAATTTGAGGGCCTGACA
  GACTTCTCAGATACGTTCAAGTTGTACCGAGGCAAGTCGGATGAAAATGAAGATCCTTCTGTGGTTGGA
  GAGTTTAAGGGCTCCTTTCGGATCTACCCTCTGCCGGATGACCCCAGCGTGCCAGCCCCTCCCAGACAG
  TTTCGGGAATTACCTGACAGCGTCCCACAGGAATGCACGGTTAGGATTTACATTGTTCGAGGCTTAGAG
  CTCCAGCCCCAGGACAACAATGGCCTGTGTGACCCTTACATAAAAATAACACTGGGCAAAAAAGTCATT
  GAAGACCGAGATCACTACATTCCCAACACTCTCAACCCAGTCTTTGGCAGGATGTACGAACTGAGCTGC
  TACTTACCTCAAGAAAAAGACCTGAAAATTTCTGTCTATGATTATGACACCTTTACCCGGGATGAAAAA
  GTAGGAGAAACAATTATTGATCTGGAAAACCGATTCCTTTCCCGCTTTGGGTCCCACTGCGGCATACCA
  GAGGAGTACTGTGTTTCTGGAGTCAATACCTGGCGAGATCAACTGAGACCAACACAGCTGCTTCAAAAT
  GTCGCCAGATTCAAAGGCTTCCCACAACCCATCCTTTCCGAAGATGGGAGTAGAATCAGATATGGAGGA
  CGAGACTACAGCTTGGATGAATTTGAAGCCAACAAAATCCTGCACCAGCACCTCGGGGCCCCTGAAGAG
  CGGCTTGCTCTTCACATCCTCAGGACTCAGGGGCTGGTCCCTGAGCACGTGGAAACAAGGACTTTGCAC
  AGCACCTTCCAGCCCAACATTTCCCAGGGAAAACTTCAGATGTGGGTGGATGTTTTCCCCAAGAGTTTG
  GGGCCACCAGGCCCTCCTTTCAACATCACACCCCGGAAAGCCAAGAAATACTACCTGCGTGTGATCATC
  TGGAACACCAAGGACGTTATCTTGGACGAGAAAAGCATCACAGGAGAGGAAATGAGTGACATCTACGTC
  AAAGGCTGGATTCCTGGCAATGAAGAAAACAAACAGAAAACAGATGTCCATTACAGATCTTTGGATGGT
  GAAGGGAATTTTAACTGGCGATTTGTTTTCCCGTTTGACTACCTTCCAGCCGAACAACTCTGTATCGTT
  GCGAAAAAAGAGCATTTCTGGAGTATTGACCAAACGGAATTTCGAATCCCACCCAGGCTGATCATTCAG
  ATATGGGACAATGACAAGTTTTCTCTGGATGACTACTTGGGTTTCCTAGAACTTGACTTGCGTCACACG
  ATCATTCCTGCAAAATCACCAGAGAAATGCAGATTGGACATGATTCCGGACCTCAAAGCCATGAACCCC
  CTTAAAGCCAAGACAGCCTCCCTCTTTGAGCAGAAGTCCATGAAAGGATGGTGGCCATGCTACGCAGAG
  AAAGATGGCGCCCGCGTAATGGCTGGGAAAGTGGAGATGACATTGGAAATCCTCAACGAGAAGGAGGCC
  GACGAGAGGCCAGCCGGGAAGGGGCGGGACGAACCCAACATGAACCCCAAGCTGGACTTACCAAATCGA
  CCAGAAACCTCCTTCCTCTGGTTCACCAACCCATGCAAGACCATGAAGTTCATCGTGTGGCGCCGCTTT
  AAGTGGGTCATCATCGGCTTGCTGTTCCTGCTTATCCTGCTGCTCTTCGTGGCCGTGCTCCTCTACTCT
  TTGCCGAACTATTTGTCAATGAAGATTGTAAAGCCAAATGTGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_013451
• Insert Size: 6186 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013451.3
• RefSeq Size: 6860 bp
• RefSeq ORF: 6186 bp
• Locus ID: 26509
• UniProt ID: Q9NZM1
• Cytogenetics: 10q23.33
• Domains: C2, DysFN, DysFC
• Protein Families: Transmembrane
• MW: 234.7 kDa
• Gene Summary: Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (1) encodes the full-length isoform (a).