H2AC4 (NM_003513) Human Untagged Clone

CAT#: SC309416

HIST1H2AB (untagged)-Human histone cluster 1, H2ab (HIST1H2AB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H2AC4
• Synonyms: H2A/m; H2AC8; H2AFM; HIST1H2AB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309416 representing NM_003513.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTGGTCGCGGCAAACAAGGCGGTAAAGCTCGCGCCAAGGCTAAGACTCGGTCTTCTCGTGCAGGT
  TTGCAGTTTCCTGTGGGCCGAGTGCACCGCCTGCTCCGCAAAGGCAACTACTCCGAGCGCGTCGGGGCT
  GGCGCGCCGGTGTATCTCGCGGCGGTGCTTGAGTACCTGACCGCCGAGATCCTGGAGCTGGCGGGCAAT
  GCGGCCCGCGACAACAAGAAGACCCGCATCATCCCGCGCCACCTGCAATTGGCCATCCGCAATGACGAG
  GAGCTTAATAAACTCTTGGGGCGTGTGACCATCGCGCAGGGTGGCGTTTTGCCTAATATTCAGGCGGTG
  CTGCTGCCTAAGAAAACTGAGAGCCATCATAAGGCCAAGGGAAAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_003513
• Insert Size: 393 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003513.2
• RefSeq Size: 477 bp
• RefSeq ORF: 393 bp
• Locus ID: 8335
• UniProt ID: P04908
• Cytogenetics: 6p22.2
• Protein Pathways: Systemic lupus erythematosus
• MW: 14.1 kDa
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]