HCAR2 (NM_177551) Human Untagged Clone

CAT#: SC309249

HCAR2 (untagged)-Human G protein-coupled receptor 109A (GPR109A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HCAR2
• Synonyms: GPR109A; HCA2; HM74a; HM74b; NIACR1; Puma-g; PUMAG
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_177551 edited
  CACCTCCTTGCTGGAGCATTCACTAGGCGAGGCGCTCCATCGGACTCACTAGCCGCACTC
  ATGAATCGGCACCATCTGCAGGATCACTTTCTGGAAATAGACAAGAAGAACTGCTGCGTG
  TTCCGAGATGACTTCATTGTCAAGGTGTTGCCGCCGGTGTTGGGGCTGGAGTTTATCTTC
  GGGCTTCTGGGCAATGGCCTTGCCCTGTGGATTTTCTGTTTCCACCTCAAGTCCTGGAAA
  TCCAGCCGGATTTTCCTGTTCAACCTGGCAGTGGCTGACTTTCTACTGATCATCTGCCTG
  CCCTTCCTGATGGACAACTATGTGAGGCGTTGGGACTGGAAGTTTGGGGACATCCCTTGC
  CGGCTGATGCTCTTCATGTTGGCTATGAACCGCCAGGGCAGCATCATCTTCCTCACGGTG
  GTGGCGGTAGACAGGTATTTCCGGGTGGTCCATCCCCACCACGCCCTGAACAAGATCTCC
  AATCGGACAGCAGCCATCATCTCTTGCCTTCTGTGGGGCATCACTATTGGCCTGACAGTC
  CACCTCCTGAAGAAGAAGATGCCGATCCAGAATGGCGGTGCAAATTTGTGCAGCAGCTTC
  AGCATCTGCCATACCTTCCAGTGGCACGAAGCCATGTTCCTCCTGGAGTTCTTCCTGCCC
  CTGGGCATCATCCTGTTCTGCTCAGCCAGAATTATCTGGAGCCTGCGGCAGAGACAAATG
  GACCGGCATGCCAAGATCAAGAGAGCCATCACCTTCATCATGGTGGTGGCCATCGTCTTT
  GTCATCTGCTTCCTTCCCAGCGTGGTTGTGCGGATCCGCATCTTCTGGCTCCTGCACACT
  TCGGGCACGCAGAATTGTGAAGTGTACCGCTCGGTGGACCTGGCGTTCTTTATCACTCTC
  AGCTTCACCTACATGAACAGCATGCTGGACCCCGTGGTGTACTACTTCTCCAGCCCATCC
  TTTCCCAACTTCTTCTCCACTTTGATCAACCGCTGCCTCCAGAGGAAGATGACAGGTGAG
  CCAGATAATAACCGCAGCACGAGCGTCGAGCTCACAGGGGACCCCAACAAAACCAGAGGC
  GCTCCAGAGGCGTTAATGGCCAACTCCGGTGAGCCATGGAGCCCCTCTTATCTGGGCCCA
  ACCTCTCCTTAAATAACCATGCCAAGAAGGGACATTGTCACCAAGAACCAGGATCTCTGG
  AGAAACAGTTGGGCTGTTGCATCGAGTAATGTCACTGGACTCGGCCTAAGGTTTCCTGGA
  ACTTCCAGATTCAGAGAATCTGATTTAGGGAAACTGTGGCAGATGAGTGGGAGACTGGTT
  GCAAGGTGTGACCGCAGGAATCCTGGAGGAACAGAGAGTAAAGCTTCTAGGCATCTGAAA
  CTTTTGCTTCATCTCTGACGCTCGCAGGACTGAAGATGGGCAAATTGTAGGCGTTTCTGC
  TGAGCAGAGTTGGAGCCAGAGATCTACTTGTGACTTGTTGGCCTTCTTCCCACATCTGCC
  TCAGACTGGGGGGGGCTCAGCTCCTGGGGTGATATCTAGCCTGCTTGTGAGCTCTAGCAG
  GGATAAGGAGAGCTGAGATTGGAGGGAATTGTGTTGCTCCTGGAGGGAGCCCAGGCATCA
  TTAAACAAGCCAGTAGGTCACCTGGCTTCCGTCGACCAATTCATCTTTCAGACAAGCTTT
  AGCAGAAATGGACTCAGGGAAGAGACTCACACGCTTTGGTTAATATCTGTGTTTCCGGTG
  GGTGTAATAGGGGATTAGCCCCAGAAGGGACTGAGCTAAACAGTGTTATTATGGGAAAGG
  AAATGGCATTGCTGCTTTCAACCAGCGACTAATGCAATCCATTCCTCTCTTGTTTATAGT
  AATCTAAGGGTTGGGCAGTTAAAACGGCTTCAGGATAGAAAGCTGTTTCCCACCTCTGTT
  TGCTTTTAACATTAAAAGGGAAATGTGCCTCTGCCCCACAGTTAGAGGGGTGCACGTTCC
  TCCTGGTTCCTTCGCTTGTGTTTCTGTACTTACCAAAAATCTACCATTTCAATAAATTTT
  GATAGGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_177551
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177551.3, NP_808219.1
• RefSeq Size: 2082 bp
• RefSeq ORF: 1092 bp
• Locus ID: 338442
• UniProt ID: Q8TDS4
• Cytogenetics: 12q24.31
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.[UniProtKB/Swiss-Prot Function]