DHX29 (NM_019030) Human Untagged Clone

CAT#: SC309164

DHX29 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 29 (DHX29)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHX29
• Synonyms: DDX29
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_019030, the custom clone sequence may differ by one or more nucleotides
  ATGGGCGGCAAGAACAAGAAACACAAGGCTCCAGCGGCCGCGGTGGTCCGGGCCGCCGTG
  TCTGCTTCCAGAGCCAAATCTGCCGAGGCTGGAATTGCCGGGGAGGCCCAAAGCAAGAAG
  CCAGTGTCCAGGCCGGCCACCGCTGCCGCTGCCGCTGCCGGCTCCAGGGAGCCCCGTGTC
  AAGCAAGGTCCAAAAATTTATAGTTTTAATTCTACAAATGATTCTAGTGGTCCTGCAAAT
  CTGGATAAATCTATTTTGAAAGTGGTAATTAATAACAAACTAGAGCAAAGAATTATTGGA
  GTGATCAATGAGCATAAAAAGCAAAATAATGACAAAGGAATGATTTCTGGAAGACTTACT
  GCCAAAAAATTGCAGGATTTATACATGGCTTTACAAGCATTTTCATTTAAGACAAAGGAC
  ATTGAAGATGCCATGACCAATACACTCTTATATGGAGGTGACCTTCATTCTGCCTTGGAT
  TGGCTCTGTTTAAACCTTTCAGATGATGCACTTCCTGAAGGATTCAGTCAGGAATTTGAA
  GAGCAGCAACCTAAAAGTAGGCCTAAATTTCAGTCTCCTCAAATACAAGCCACTATTTCA
  CCTCCATTGCAACCTAAAACAAAAACATATGAAGAGGACCCTAAGAGTAAGCCAAAAAAG
  GAAGAAAAAAATATGGAAGTAAATATGAAAGAGTGGATTTTACGATATGCTGAACAACAA
  AATGAAGAAGAAAAGAATGAGAATTCTAAAAGTTTAGAAGAGGAGGAAAAATTTGACCCT
  AATGAAAGGTACTTACATCTTGCAGCAAAACTGCTGGATGCAAAAGAACAAGCAGCTACC
  TTTAAACTAGAAAAAAACAAGCAAGGCCAAAAAGAGGCTCAGGAAAAAATAAGGAAATTT
  CAAAGAGAAATGGAAACTTTAGAAGACCATCCAGTATTTAACCCAGCCATGAAGATTTCA
  CATCAACAAAATGAAAGGAAAAAGCCTCCTGTAGCCACAGAAGGAGAAAGTGCATTGAAT
  TTTAATTTATTTGAAAAATCTGCAGCTGCTACTGAAGAAGAGAAAGATAAAAAGAAAGAA
  CCTCATGATGTAAGAAATTTTGACTATACTGCTCGAAGTTGGACTGGAAAATCTCCCAAA
  CAATTTCTGATTGATTGGGTCAGGAAGAATCTTCCCAAGAGTCCAAATCCTTCCTTTGAA
  AAAGTTCCAGTAGGTAGATACTGGAAATGTAGGGTTAGGGTAATCAAGTCTGAAGATGAT
  GTCCTGGTAGTATGCCCTACAATCTTAACAGAAGATGGCATGCAAGCTCAGCACCTGGGA
  GCTACTTTAGCCCTTTACCGTTTAGTTAAAGGGCAGTCAGTTCATCAGTTACTTCCTCCC
  ACTTACCGAGATGTTTGGCTGGAGTGGAGTGATGCAGAAAAGAAAAGGGAAGAATTAAAT
  AAAATGGAAACCAATAAACCACGTGATCTTTTTATTGCCAAACTTCTGAATAAACTGAAA
  CAGCAGCAACAGCAGCAACAACAGCATTCTGAAAATAAGAGAGAAAACTCTGAAGATCCC
  GAGGAATCTTGGGAAAATTTAGTTTCGGATGAGGATTTTTCTGCACTGTCCTTGGAATCA
  GCAAATGTGGAAGATTTGGAACCTGTTAGAAACCTCTTTAGAAAGTTGCAAAGCACACCT
  AAGTATCAGAAACTTCTAAAGGAAAGACAACAGCTACCTGTATTTAAACATCGGGACTCA
  ATTGTTGAAACTCTTAAAAGGCATCGGGTAGTGGTTGTGGCAGGTGAAACAGGGAGTGGT
  AAAAGTACTCAGGTACCACATTTTCTATTGGAAGATTTGCTTCTAAATGAGTGGGAAGCA
  AGTAAATGTAACATTGTCTGTACCCAACCCCGAAGAATCTCAGCAGTTAGTTTAGCCAAC
  AGAGTATGTGATGAATTGGGCTGTGAAAATGGACCTGGAGGAAGGAATTCCTTGTGTGGA
  TATCAGATCCGGATGGAATCTCGAGCTTGTGAATCTACCAGGTTACTCTATTGTACAACA
  GGGGTTTTGCTAAGGAAACTTCAAGAAGATGGTCTTCTAAGTAATGTGTCTCATGTTATT
  GTAGATGAGGTTCATGAAAGAAGTGTCCAGTCAGACTTCCTACTAATTATCTTGAAGGAA
  ATTTTACAGAAACGTTCTGATCTACACTTGATTCTAATGAGTGCCACTGTGGACAGCGAA
  AAATTTTCTACATATTTCACACACTGCCCCATTCTCAGAATTTCAGGAAGAAGTTATCCT
  GTTGAGGTTTTTCATCTTGAAGATATAATAGAAGAAACAGGCTTTGTACTGGAAAAAGAC
  TCAGAATATTGTCAGAAATTTCTGGAAGAGGAAGAAGAAGTAACCATTAATGTTACAAGC
  AAAGCAGGGGGAATAAAAAAATATCAGGAATACATCCCAGTTCAGACTGGAGCACATGCT
  GATTTAAATCCATTTTACCAAAAGTACAGCAGCCGCACTCAGCATGCTATTCTATACATG
  AATCCTCATAAAATCAACCTGGATCTCATTTTGGAACTTCTTGCATACTTAGATAAAAGT
  CCCCAATTCAGAAATATTGAAGGAGCAGTATTGATCTTTTTACCAGGACTTGCTCATATT
  CAGCAGTTGTATGATCTTCTATCAAATGATAGAAGATTTTATTCTGAACGATATAAAGTG
  ATAGCTCTGCATTCTATTCTTTCAACCCAAGATCAAGCTGCAGCATTCACACTTCCCCCT
  CCAGGAGTCAGGAAGATTGTTTTAGCAACCAATATTGCAGAGACGGGTATCACTATTCCT
  GATGTTGTATTTGTAATTGATACTGGAAGAACAAAAGAAAATAAGTACCATGAAAGCAGT
  CAGATGAGTTCTTTGGTGGAGACGTTTGTCAGTAAAGCCAGTGCTTTGCAGCGCCAGGGA
  AGAGCTGGGCGGGTCAGAGATGGCTTCTGTTTCCGAATGTACACAAGAGAAAGATTTGAA
  GGCTTTATGGATTATTCTGTTCCTGAAATCTTACGTGTACCTTTGGAGGAATTATGCCTT
  CATATTATGAAATGTAATCTTGGTTCTCCTGAAGATTTCCTCTCCAAAGCCTTAGATCCT
  CCTCAGCTCCAAGTGATCAGCAATGCAATGAATTTGCTCCGAAAAATTGGAGCTTGTGAA
  TTAAATGAGCCTAAACTGACTCCGTTGGGCCAACACCTTGCAGCTTTACCTGTGAATGTC
  AAGATTGGCAAGATGCTTATTTTTGGTGCCATATTTGGCTGCCTTGACCCAGTGGCAACA
  CTAGCTGCAGTTATGACAGAGAAGTCTCCTTTTACCACACCAATTGGTCGAAAAGATGAA
  GCAGATCTTGCAAAATCAGCTTTGGCCATGGCGGATTCAGACCACCTGACGATCTACAAT
  GCATATCTAGGATGGAAGAAAGCACGACAAGAAGGAGGTTATCGTTCTGAAATCACATAC
  TGCCGGAGGAACTTTCTTAATAGAACATCACTGTTAACCCTAGAGGATGTAAAGCAGGAG
  TTAATAAAGTTGGTTAAGGCAGCAGGATTTTCATCTTCCACAACTTCTACCAGCTGGGAA
  GGAAACAGAGCCTCACAGACCCTCTCATTCCAAGAAATTGCCCTTCTTAAAGCTGTACTG
  GTGGCTGGACTGTATGACAATGTGGGGAAGATAATCTATACAAAGTCAGTGGATGTTACA
  GAAAAATTGGCTTGCATTGTGGAGACGGCCCAAGGCAAAGCACAAGTACACCCATCCTCA
  GTAAATCGAGATTTGCAAACTCATGGATGGCTCTTATACCAGGAGAAGATAAGGTATGCC
  AGAGTGTATTTGAGAGAAACTACCCTAATAACCCCTTTTCCAGTTTTACTTTTTGGTGGT
  GATATAGAAGTTCAGCACCGAGAACGTCTTCTTTCTATTGATGGCTGGATCTATTTTCAG
  GCCCCTGTAAAGATAGCTGTCATTTTCAAGCAGCTGAGAGTTCTCATTGATTCAGTTTTA
  AGAAAAAAGCTTGAAAATCCAAAGATGTCCCTTGAAAATGACAAGATTCTGCAGATCATT
  ACGGAATTGATAAAAACAGAGAATAACTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_019030
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019030.2, NP_061903.2
• RefSeq Size: 4491 bp
• RefSeq ORF: 4110 bp
• Locus ID: 54505
• UniProt ID: Q7Z478
• Cytogenetics: 5q11.2
• Gene Summary: This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1).