GLI3 (NM_000168) Human Untagged Clone

CAT#: SC309064

GLI3 (untagged)-Human GLI family zinc finger 3 (GLI3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLI3
• Synonyms: ACLS; GCPS; GLI3-190; GLI3FL; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000168 edited
  ATGGAGGCCCAGTCCCACAGCTCCACGACCACTGAAAAGAAAAAAGTTGAGAATTCCATA
  GTGAAGTGCTCCACTCGAACAGATGTGAGCGAGAAAGCCGTTGCCTCCAGCACCACTTCT
  AATGAGGATGAAAGTCCTGGACAGACTTATCACAGAGAGAGAAGAAACGCAATCACTATG
  CAGCCACAGAATGTCCAGGGGCTCAGCAAAGTCAGTGAGGAACCTTCAACATCGAGTGAC
  GAGAGGGCCTCATTGATCAAGAAAGAGATCCATGGGTCCCTGCCACACGTGGCGGAGCCC
  TCTGTGCCGTACCGCGGGACGGTGTTTGCCATGGACCCCAGGAATGGTTACATGGAGCCC
  CACTACCACCCTCCTCATCTTTTCCCTGCCTTCCATCCTCCTGTACCAATTGATGCCAGA
  CATCATGAGGGCCGTTACCATTACGATCCATCTCCGATTCCTCCATTGCATATGACTTCC
  GCCTTATCTAGTAGCCCTACGTATCCGGACCTGCCCTTCATTAGGATCTCCCCACACCGG
  AACCCCGCTGCTGCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACATTAATCCCTAC
  ATGGACTATATCCGCTCCTTGCACAGCAGCCCATCGCTCTCCATGATCTCAGCAACCCGT
  GGGCTGAGCCCTACAGATGCGCCCCATGCAGGAGTCAGCCCAGCAGAATACTATCATCAG
  ATGGCCCTGCTAACTGGCCAGCGCAGCCCCTATGCAGACATTATTCCCTCAGCTGCCACC
  GCCGGCACGGGGGCCATCCACATGGAATATCTTCATGCTATGGATAGCACCAGATTCTCC
  AGCCCCAGGCTGTCAGCCAGGCCGAGCCGAAAACGTACACTGTCCATATCACCACTCTCC
  GATCATAGCTTTGACCTTCAGACCATGATAAGGACGTCTCCCAACTCCTTGGTCACGATT
  CTCAATAATTCCCGTAGCAGCTCTTCAGCAAGTGGCTCCTATGGTCACTTATCTGCAAGT
  GCAATCAGCCCTGCCTTGAGCTTCACCTACTCTTCCGCGCCCGTCTCTCTCCACATGCAT
  CAGCAGATCCTAAGCCGACAACAGAGCTTAGGTTCAGCCTTTGGACACAGCCCTCCACTC
  ATCCACCCTGCCCCAACTTTTCCAACACAGAGGCCTATTCCAGGGATCCCTACGGTTCTG
  AACCCCGTCCAGGTCAGCTCCGGCCCTTCTGAGTCCTCACAGAACAAGCCCACGAGTGAG
  TCTGCAGTGAGCAGCACTGGTGACCCGATGCACAACAAGAGGTCCAAGATCAAACCCGAT
  GAAGACCTCCCCAGCCCAGGGGCTCGGGGGCAGCAGGAACAGCCCGAAGGAACAACCCTT
  GTCAAGGAGGAAGGGGACAAAGATGAAAGCAAACAGGAGCCTGAAGTCATCTATGAGACA
  AACTGCCACTGGGAAGGCTGCGCGAGGGAGTTCGACACCCAAGAGCAGCTTGTGCACCAT
  ATAAATAACGACCATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGC
  TCAAGAGAGCAGAAACCCTTCAAAGCCCAGTATATGTTGGTAGTGCATATGAGAAGACAC
  ACGGGCGAGAAGCCTCACAAATGCACTTTTGAAGGTTGCACAAAGGCCTACTCGAGACTA
  GAAAACTTGAAAACACACTTGAGATCTCACACTGGAGAGAAACCATACGTCTGTGAGCAC
  GAAGGTTGCAACAAGGCTTTCTCAAATGCCTCTGATCGCGCCAAACACCAAAACAGAACG
  CATTCCAATGAGAAACCATATGTGTGCAAAATCCCAGGCTGCACTAAGCGTTACACAGAC
  CCAAGCTCCCTCCGGAAACATGTGAAGACAGTGCATGGCCCAGAGGCTCATGTCACCAAG
  AAGCAGCGAGGGGACATCCATCCTCGGCCGCCACCCCCGAGAGATTCCGGCAGCCATTCA
  CAGTCCAGGTCGCCTGGCCGACCGACTCAGGGAGCCCTTGGTGAGCAGCAGGACCTCAGC
  AACACTACCTCAAAGCGGGAAGAATGCCTCCAGGTGAAAACCGTCAAGGCAGAGAAGCCA
  ATGACATCTCAGCCAAGCCCTGGTGGTCAGTCTTCATGCAGCAGCCAACAGTCCCCCATC
  AGCAACTATTCCAACAGTGGGCTCGAGCTTCCTCTGACCGATGGAGGTAGTATAGGAGAC
  CTCAGTGCCATCGATGAAACCCCAATCATGGACTCAACCATTTCCACTGCAACCACAGCC
  CTTGCTTTGCAAGCCAGGAGAAACCCGGCAGGGACCAAATGGATGGAGCACGTAAAACTA
  GAAAGGCTAAAACAAGTGAATGGAATGTTTCCGCGACTGAACCCCATTCTACCCCCTAAA
  GCCCCTGCGGTCTCTCCTCTCATAGGAAATGGCACACAGTCCAACAACACCTGCAGCTTG
  GGTGGGCCCATGACGCTTCTCCCGGGCAGAAGCGACCTCTCTGGGGTGGACGTCACTATG
  CTGAACATGCTCAACAGAAGGGACAGCAGCGCCAGCACCATCAGCTCGGCCTACCTGAGC
  AGCCGCCGCTCCTCAGGGATCTCGCCCTGCTTCTCCAGCCGCCGCTCCAGCGAGGCGTCA
  CAGGCCGAGGGCCGGCCGCAGAACGTGAGCGTGGCCGACTCCTACGACCCCATCTCCACC
  GACGCCTCGCGCCGCTCCAGCGAAGCCAGCCAGAGCGACGGCCTGCCCAGCCTGCTCAGC
  CTCACGCCCGCCCAGCAGTACCGCCTCAAGGCCAAGTACGCGGCTGCCACAGGAGGGCCG
  CCGCCGACGCCCCTGCCCAACATGGAGAGGATGAGCCTGAAGACGCGCCTGGCGCTGCTC
  GGGGATGCCCTCGAGCCTGGCGTGGCCCTGCCTCCAGTTCATGCCCCGAGGAGGTGCAGC
  GACGGGGGAGCCCACGGCTACGGGCGGCGCCACCTGCAGCCGCACGATGCGCTGGGCCAC
  GGCGTGAGGAGGGCCAGCGACCCGGTGCGGACAGGCTCCGAGGGCCTGGCCCTGCCTCGT
  GTGCCGCGCTTCAGCAGCCTCAGCAGCTGCAACCCCCCGGCGATGGCCACGTCCGCGGAG
  AAGCGCAGTCTCGTGCTTCAGAATTACACGCGGCCCGAGGGCGGCCAGTCCCGAAACTTC
  CACTCGTCCCCCTGTCCTCCCAGCATCACCGAGAACGTCACCCTGGAGTCCCTGACCATG
  GACGCTGATGCCAACCTGAACGATGAGGATTTCCTGCCGGACGACGTGGTGCAGTATTTA
  AATTCCCAGAACCAAGCAGGGTACGAGCAGCACTTCCCCAGCGCCCTCCCGGACGACAGC
  AAAGTGCCCCACGGGCCCGGTGACTTTGACGCGCCCGGGCTGCCAGACAGCCACGCTGGC
  CAGCAGTTCCATGCCCTCGAGCAGCCCTGCCCCGAGGGCAGCAAAACCGACCTGCCCATT
  CAGTGGAACGAAGTCAGCTCCGGAAGCGCCGACCTGTCCTCCTCCAAGCTCAAGTGTGGG
  CCGCGGCCCGCTGTGCCGCAGACTCGCGCCTTTGGGTTCTGCAACGGCATGGTCGTCCAC
  CCGCAGAACCCCTTGAGGAGCGGGCCTGCTGGGGGCTATCAGACCCTCGGGGAGAACAGC
  AACCCCTACGGTGGCCCAGAGCACTTGATGCTCCACAACAGCCCCGGAAGTGGCACCAGT
  GGAAACGCCTTCCATGAACAGCCCTGTAAGGCCCCGCAGTATGGGAACTGTCTCAACAGG
  CAGCCAGTGGCCCCTGGTGCACTCGACGGTGCCTGTGGTGCCGGGATTCAAGCCTCAAAG
  CTGAAGAGCACCCCCATGCAAGGGAGCGGGGGCCAGCTGAATTTCGGCCTGCCGGTAGCG
  CCAAATGAGTCAGCTGGCAGCATGGTGAATGGCATGCAGAACCAGGACCCAGTGGGACAG
  GGGTACCTGGCTCACCAGCTCCTCGGCGACAGCATGCAGCACCCGGGGGCAGGCCGCCCC
  GGTCAGCAGATGCTTGGGCAGATTAGTGCTACCTCACACATCAACATCTACCAAGGGCCA
  GAGAGCTGCCTGCCAGGGGCTCACGGCATGGGCAGCCAGCCGTCAAGCTTGGCAGTTGTC
  AGGGGCTACCAGCCATGTGCCAGCTTTGGGGGCAGCAGGCGCCAGGCTATGCCGAGGGAC
  AGCCTTGCTCTGCAGTCAGGACAGCTCAGTGACACAAGTCAGACCTGCAGGGTGAATGGT
  ATCAAGATGGAGATGAAAGGGCAGCCCCATCCGCTGTGCTCTAATCTGCAGAATTACTCT
  GGTCAGTTCTATGACCAAACCGTGGGCTTCAGTCAGCAAGACACGAAAGCTGGTTCATTC
  TCTATTTCAGACGCCAGCTGCCTGCTACAGGGGACCAGCGCCAAAAACTCTGAGTTACTT
  TCCCCAGGTGCTAATCAGGTGACAAGCACAGTGGACAGCCTCGACAGCCATGACCTGGAA
  GGGGTACAGATTGACTTCGATGCCATCATAGACGATGGGGACCACTCCAGCCTGATGTCG
  GGGGCCCTGAGCCCAAGTATCATTCAGAACCTTTCCCATAGCTCCTCCCGCCTCACCACG
  CCTCGGGCGTCCCTCCCATTCCCAGCGCTGTCCATGAGCACCACCAACATGGCTATCGGG
  GACATGAGTTCTTTGCTGACCTCCCTAGCGGAAGAAAGCAAATTCCTTGCAGTTATGCAA
  TAG
  
• Restriction Sites: Please inquire
• ACCN: NM_000168
• Insert Size: 4800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000168.2, NP_000159.2
• RefSeq Size: 5054 bp
• RefSeq ORF: 4743 bp
• Locus ID: 2737
• UniProt ID: P10071
• Cytogenetics: 7p14.1
• Domains: zf-C2H2
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Gene Summary: This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]