DNA Ligase IV (LIG4) (NM_206937) Human Untagged Clone

SKU SC308332

LIG4 (untagged)-Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNA Ligase IV
• Synonyms: LIG4S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308332 representing NM_206937.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCTGCCTCACAAACTTCACAAACTGTTGCATCTCACGTTCCTTTTGCAGATTTGTGTTCAACTTTA; GAACGAATACAGAAAAGTAAAGGACGTGCAGAAAAAATCAGACACTTCAGGGAATTTTTAGATTCTTGG; AGAAAATTTCATGATGCTCTTCATAAGAACCACAAAGATGTCACAGACTCTTTTTATCCAGCAATGAGA; CTAATTCTTCCTCAGCTAGAAAGAGAGAGAATGGCCTATGGAATTAAAGAAACTATGCTTGCTAAGCTT; TATATTGAGTTGCTTAATTTACCTAGAGATGGAAAAGATGCCCTCAAACTTTTAAACTACAGAACACCC; ACTGGAACTCATGGAGATGCTGGAGACTTTGCAATGATTGCATATTTTGTGTTGAAGCCAAGATGTTTA; CAGAAAGGAAGTTTAACCATACAGCAAGTAAACGACCTTTTAGACTCAATTGCCAGCAATAATTCTGCT; AAAAGAAAAGACCTAATAAAAAAGAGCCTTCTTCAACTTATAACTCAGAGTTCAGCACTTGAGCAAAAG; TGGCTTATACGGATGATCATAAAGGATTTAAAGCTTGGTGTTAGTCAGCAAACTATCTTTTCTGTTTTT; CATAATGATGCTGCTGAGTTGCATAATGTCACTACAGATCTGGAAAAAGTCTGTAGGCAACTGCATGAT; CCTTCTGTAGGACTCAGTGATATTTCTATCACTTTATTTTCTGCATTTAAACCAATGCTAGCTGCTATT; GCAGATATTGAGCACATTGAGAAGGATATGAAACATCAGAGTTTCTACATAGAAACCAAGCTAGATGGT; GAACGTATGCAAATGCACAAAGATGGAGATGTATATAAATACTTCTCTCGAAATGGATATAACTACACT; GATCAGTTTGGTGCTTCTCCTACTGAAGGTTCTCTTACCCCATTCATTCATAATGCATTCAAAGCAGAT; ATACAAATCTGTATTCTTGATGGTGAGATGATGGCCTATAATCCTAATACACAAACTTTCATGCAAAAG; GGAACTAAGTTTGATATTAAAAGAATGGTAGAGGATTCTGATCTGCAAACTTGTTATTGTGTTTTTGAT; GTATTGATGGTTAATAATAAAAAGCTAGGGCATGAGACTCTGAGAAAGAGGTATGAGATTCTTAGTAGT; ATTTTTACACCAATTCCAGGTAGAATAGAAATAGTGCAGAAAACACAAGCTCATACTAAGAATGAAGTA; ATTGATGCATTGAATGAAGCAATAGATAAAAGAGAAGAGGGAATTATGGTAAAACAACCTCTATCCATC; TACAAGCCAGACAAAAGAGGTGAAGGGTGGTTAAAAATTAAACCAGAGTATGTCAGTGGACTAATGGAT; GAATTGGACATTTTAATTGTTGGAGGATATTGGGGTAAAGGATCACGGGGTGGAATGATGTCTCATTTT; CTGTGTGCAGTAGCAGAGAAGCCCCCTCCTGGTGAGAAGCCATCTGTGTTTCATACTCTCTCTCGTGTT; GGGTCTGGCTGCACCATGAAAGAACTGTATGATCTGGGTTTGAAATTGGCCAAGTATTGGAAGCCTTTT; CATAGAAAAGCTCCACCAAGCAGCATTTTATGTGGAACAGAGAAGCCAGAAGTATACATTGAACCTTGT; AATTCTGTCATTGTTCAGATTAAAGCAGCAGAGATCGTACCCAGTGATATGTATAAAACTGGCTGCACC; TTGCGTTTTCCACGAATTGAAAAGATAAGAGATGACAAGGAGTGGCATGAGTGCATGACCCTGGACGAC; CTAGAACAACTTAGGGGGAAGGCATCTGGTAAGCTCGCATCTAAACACCTTTATATAGGTGGTGATGAT; GAACCACAAGAAAAAAAGCGGAAAGCTGCCCCAAAGATGAAGAAAGTTATTGGAATTATTGAGCACTTA; AAAGCACCTAACCTTACTAACGTTAACAAAATTTCTAATATATTTGAAGATGTAGAGTTTTGTGTTATG; AGTGGAACAGATAGCCAGCCAAAGCCTGACCTGGAGAACAGAATTGCAGAATTTGGTGGTTATATAGTA; CAAAATCCAGGCCCAGACACGTACTGTGTAATTGCAGGGTCTGAGAACATCAGAGTGAAAAACATAATT; TTGTCAAATAAACATGATGTTGTCAAGCCTGCATGGCTTTTAGAATGTTTTAAGACCAAAAGCTTTGTA; CCATGGCAGCCTCGCTTTATGATTCATATGTGCCCATCAACCAAAGAACATTTTGCCCGTGAATATGAT; TGCTATGGTGATAGTTATTTCATTGATACAGACTTGAACCAACTGAAGGAAGTATTCTCAGGAATTAAA; AATTCTAACGAGCAGACTCCTGAAGAAATGGCTTCTCTGATTGCTGATTTAGAATATCGGTATTCCTGG; GATTGCTCTCCTCTCAGTATGTTTCGACGCCACACCGTTTATTTGGACTCGTATGCTGTTATTAATGAC; CTGAGTACCAAAAATGAGGGGACAAGGTTAGCTATTAAAGCCTTGGAGCTTCGGTTTCATGGAGCAAAA; GTAGTTTCTTGTTTAGCTGAGGGAGTGTCTCATGTAATAATTGGGGAAGATCATAGTCGTGTTGCAGAT; TTTAAAGCTTTTAGAAGAACTTTTAAGAGAAAGTTTAAAATCCTAAAAGAAAGTTGGGTAACTGATTCA; ATAGACAAGTGTGAATTACAAGAAGAAAACCAGTATTTGATTTAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_206937
• Insert Size: 2736 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_206937.1
• RefSeq Size: 3994 bp
• RefSeq ORF: 2736 bp
• Locus ID: 3981
• UniProt ID: P49917
• Cytogenetics: 13q33.3
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• MW: 104 kDa
• Summary: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 and 5-10 all encode the same isoform (1).