LRRC50 (DNAAF1) (NM_178452) Human Untagged Clone

CAT#: SC307182

DNAAF1 (untagged)-Human leucine rich repeat containing 50 (LRRC50)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNAAF1
• Synonyms: CILD13; DAU1; LRRC50; ODA7; swt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC307182 representing NM_178452.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCACCCTGAGCCCTCGGAGCCTGCGACAGGTGGTGCAGCAGAGCTGGATTGCGCGCAGGAGCCCGGC
  GTGGAGGAGTCTGCGGGTGACCACGGGAGCGCAGGCCGAGGGGGCTGCAAGGAAGAAATTAATGATCCT
  AAGGAAATATGTGTGGGTTCTTCTGACACATCCTACCACAGCCAGCAGAAACAGAGTGGTGATAATGGG
  TCAGGTGGTCACTTCGCACACCCAAGAGAAGACAGGGAAGATCGGGGCCCCAGAATGACTAAAAGTTCC
  CTGCAAAAACTCTGCAAGCAGCACAAGCTTTATATTACCCCAGCATTGAATGATACGCTGTATTTACAC
  TTTAAAGGTTTTGATCGCATTGAGAACCTGGAAGAGTACACAGGGCTGCGCTGTCTCTGGCTGCAGAGC
  AATGGAATACAGAAAATCGAAAACCTGGAGGCCCAAACTGAGTTGCGTTGCCTCTTCTTGCAAATGAAC
  TTGCTCCGTAAAATTGAGAACCTGGAACCTCTGCAGAAACTGGATGCTCTTAACCTCAGCAACAATTAC
  ATCAAGACCATTGAAAACCTCTCCTGCCTCCCAGTCCTGAACACATTGCAGATGGCCCACAATCACCTG
  GAGACCGTGGAGGACATTCAGCATCTACAAGAGTGTTTGAGGCTTTGTGTCCTTGACCTTTCGCACAAC
  AAGCTGAGTGACCCGGAGATCCTGAGCATTCTGGAAAGCATGCCCGATTTGCGTGTACTGAATTTGATG
  GGAAACCCGGTTATCAGACAGATTCCTAATTACAGAAGGACAGTCACTGTACGACTAAAGCACTTAACA
  TACCTGGATGATAGACCAGTGTTTCCAAAGGACAGAGCTTGTGCGGAGGCCTGGGCTAGGGGAGGGTAC
  GCAGCTGAAAAGGAGGAGAGACAGCAGTGGGAGAGCAGGGAGCGGAAGAAGATCACAGACAGCATTGAA
  GCCTTGGCCATGATCAAGCAGCGGGCAGAGGAGAGGAAAAGACAGAGAGAGAGTCAAGAGAGAGGGGAG
  ATGACATCTTCAGATGATGGTGAGAATGTGCCCGCCAGTGCGGAAGGCAAGGAGGAGCCTCCCGGGGAC
  AGAGAAACAAGGCAGAAGATGGAGCTATTTGTTAAGGAAAGCTTTGAGGCCAAGGACGAGCTCTGCCCG
  GAAAAGCCAAGTGGAGAGGAGCCGCCTGTGGAGGCTAAAAGAGAGGATGGAGGTCCAGAGCCAGAGGGG
  ACCCTCCCAGCTGAGACCCTGCTACTGTCGTCACCTGTGGAGGTTAAAGGAGAGGACGGAGATGGAGAG
  CCAGAGGGGACCCTCCCAGCTGAGGCCCCACCACCCCCGCCACCTGTGGAGGTTAAAGGAGAGGATGGA
  GATCAAGAGCCAGAGGGGACCCTCCCAGCTGAGACCCTGCTACTGTCACCGCCTGTGAAGGTTAAAGGA
  GAGGATGGAGATCGAGAGCCAGAGGGGACCCTCCCAGCTGAGGCCCCACCACCACCGCCCCTGGGAGCT
  GCCAGGGAAGAACCGACTCCCCAGGCTGTGGCCACTGAGGGTGTATTCGTTACAGAACTTGATGGAACG
  AGAACGGAAGATTTAGAAACCATTAGACTGGAGACAAAGGAGACATTCTGCATTGATGACCTACCTGAC
  TTGGAAGATGATGATGAAACAGGCAAATCTCTGGAAGACCAGAATATGTGCTTTCCGAAGATTGAGGTC
  ATCTCGAGCTTGAGTGATGACAGTGACCCTGAACTGGACTACACGTCACTCCCTGTGCTGGAAAACCTC
  CCCACAGACACTCTGTCAAATATATTTGCAGTCTCTAAAGACACCTCAAAGGCGGCTCGGGTGCCCTTC
  ACAGACATCTTTAAAAAAGAAGCTAAGAGGGACTTGGAAATCCGAAAACAAGACACCAAGTCCCCAAGA
  CCCCTGATCCAGGAGCTCAGCGACGAGGACCCCTCTGGCCAGCTACTGATGCCCCCCACCTGCCAAAGA
  GATGCTGCACCACTCACTTCCAGTGGAGACAGGGACAGCGACTTCCTTGCAGCCTCTTCTCCGGTGCCG
  ACTGAGAGCGCCGCCACACCCCCAGAGACGTGTGTCGGAGTTGCCCAGCCCAGCCAAGCTCTGCCCACG
  TGGGACCTCACTGCATTCCCAGCACCGAAAGCATCATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_178452
• Insert Size: 2178 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178452.5
• RefSeq Size: 2452 bp
• RefSeq ORF: 2178 bp
• Locus ID: 123872
• UniProt ID: Q8NEP3
• Cytogenetics: 16q24.1
• MW: 80 kDa
• Gene Summary: The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).