M-CSF (CSF1) (NM_172210) Human Untagged Clone

CAT#: SC306739

CSF1 (untagged)-Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: M-CSF
• Synonyms: CSF-1; MCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172210 edited
  GAGGGCTGGCCAGTGAGGCTCGGCCCGGGGAAAGTGAAAGTTTGCCTGGGTCCTCTCGGC
  GCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTC
  CGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCA
  GCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGC
  TCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAG
  TACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGT
  CAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGAT
  CCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATG
  CGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTG
  AGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGACAAGGCCTGCGTCCGAACT
  TTCTATGAGACACCTCTCCAGTTGCTGGAGAAGGTCAAGAATGTCTTTAATGAAACAAAG
  AATCTCCTTGACAAGGACTGGAATATTTTCAGCAAGAACTGCAACAACAGCTTTGCTGAA
  TGCTCCAGCCAAGATGTGGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATC
  CCTAGCAGTGACCCGGCCTCTGTCTCCCCTCATCAGCCCCTCGCCCCCTCCATGGCCCCT
  GTGGCTGGCTTGACCTGGGAGGACTCTGAGGGAACTGAGGGCAGCTCCCTCTTGCCTGGT
  GAGCAGCCCCTGCACACAGTGGATCCAGGCAGTGCCAAGCAGCGGCCACCCAGGAGCACC
  TGCCAGAGCTTTGAGCCGCCAGAGACCCCAGTTGTCAAGGACAGCACCATCGGTGGCTCA
  CCACAGCCTCGCCCCTCTGTCGGGGCCTTCAACCCCGGGATGGAGGATATTCTTGACTCT
  GCAATGGGCACTAATTGGGTCCCAGAAGAAGCCTCTGGAGAGGCCAGTGAGATTCCCGTA
  CCCCAAGGGACAGAGCTTTCCCCCTCCAGGCCAGGAGGGGGCAGCATGCAGACAGAGCCC
  GCCAGACCCAGCAACTTCCTCTCAGCATCTTCTCCACTCCCTGCATCAGCAAAGGGCCAA
  CAGCCGGCAGATGTAACTGGCCATGAGAGGCAGTCCGAGGGATCCTCCAGCCCGCAGCTC
  CAGGAGTCTGTCTTCCACCTGCTGGTGCCCAGTGTCATCCTGGTCTTGCTGGCCGTCGGA
  GGCCTCTTGTTCTACAGGTGGAGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCT
  CCCTTGGAGCAACCAGAGGGCAGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCA
  GTGTAGAGGGAATTCTAAGCTGGACGCACAGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172210 unedited
  NAAGTTAAATTTTGTATACGACTCATATAGGCGGCCGCGACATTCGCACGAGGGAAGGGN
  CNNTGGCCAGTGAGGGGCTCGGCCCGGGGNAAAGTGAAAGTTTGCCTGGGTCCTCTCGGC
  GCCAGAGCCGCTCTCCGCATCCCAGGACAGCGGTGCGGCCCTCGGCCGGGGCGCCCACTC
  CGCAGCAGCCAGCGAGCGAGCGAGCGAGCGAGGGCGGCCGACGCGCCCGGCCGGGACCCA
  GCTGCCCGTATGACCGCGCCGGGCGCCGCCGGGCGCTGCCCTCCCACGACATGGCTGGGC
  TCCCTGCTGTTGTTGGTCTGTCTCCTGGCGAGCAGGAGTATCACCGAGGAGGTGTCGGAG
  TACTGTAGCCACATGATTGGGAGTGGACACCTGCAGTCTCTGCAGCGGCTGATTGACAGT
  CAGATGGAGACCTCGTGCCAAATTACATTTGAGTTTGTAGACCAGGAACAGTTGAAAGAT
  CCAGTGTGCTACCTTAAGAAGGCATTTCTCCTGGTACAAGACATAATGGAGGACACCATG
  CGCTTCAGAGATAACACCCCCAATGCCATCGCCATTGTGCAGCTGCAGGAACTCTCTTTG
  AGGCTGAAGAGCTGCTTCACCAAGGATTATGAAGAGCATGANCAGGCCTGCGTCCGAACT
  TTCTATGAGACACCTCTCCAGTTGCTGGAGGAGGTCAAGAATGTCTTTAATGAAACAAGA
  ATCTCCTTGACAGGACTGGAATATTTTCAGCAAGAACTGCAACANCAGCTTTGCTGAATG
  CTCCAGCCAAGAATGTGTGACCAAGCCTGATTGCAACTGCCTGTACCCCAAAGCCATCCC
  TAGCAGTGACCCGGCCTTTGGTTTCCCTTATAAGGCCCTTGCCCCCTTCATGACCCCTGG
  GGCTG
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_172210 unedited
  TCTCTGCCCAGCGCCATGGCTGGTCTTGCTGGCCGTCGGAAGCCTCTTGTTCTACAGGTG
  GAGGCGGCGGAGCCATCAAGAGCCTCAGAGAGCGGATTCTCCCTTGGAGCAACCAGAGGG
  CAGCCCCCTGACTCAGGATGACAGACAGGTGGAACTGCCAGTGTAGAGGGAATTCTAAGC
  TGGACGCACAGAACAGTCTCTCCGTGGGAGGAGACATTATGGGGCGTCCACCACCACCCC
  TCCCTGGCCATCCTCCTGGAATGTGGTCTGCCCTCCACCAGAGCTCCTGCCTGCCAGGAC
  TGGACCAGAGCAGCCAGGCTGGGGCCCCTCTGTCTCAACCCGCAGACCCTTGACTGAATG
  AGAGAGGCCAGAGGATGCTCCCCATGCTGCCACTATTTATTGTGAGCCCTGGAGGCTCCC
  ATGTGCTTGAGGAAGGCTGGTGAGCCCGGCTCANGACCCTCTTCCCTCAGGGGCTGCACC
  CTCCTCTCACTCNCTTCCATGCCGGAACCCAGGCCAGGGACCCACCGGCCTGTGGTTTGT
  GGGAAAGCANGGTGGACGCTGANGAGTGAAAGAACCCTGCACCCAGAGGGCCTGCCTGGT
  GCCAAAGTATCCCAGCCTGGACAGGCATGGACCTGTCTCCAGAGAGAGGACCTGAAGTTC
  GTGGGGCGGGACAGCGTCCGGCCGATTTCCCGAAAAGTGTGCAACCCTGAAACGGGAAAA
  GGAGCCTTTTGACCTGCTGGTCTGCACTGACCGCCTGAAAGGTCTACCCCCTCGGCTAAC
  CTAAATGCCCTGTGCTTGTTGGCAGGCCCCAAAGGGAAGCCCGCCCCTGCCCTAAGACCT
  GCCTGACCTCGCCATGATGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_172210
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172210.1, NP_757349.1
• RefSeq Size: 1519 bp
• RefSeq ORF: 1317 bp
• Locus ID: 1435
• UniProt ID: P09603
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
• Gene Summary: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1, resulting in a shorter isoform (b) compared to isoform a.