BDNF (NM_170734) Human Untagged Clone

CAT#: SC306691

BDNF (untagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BDNF
• Synonyms: ANON2; BULN2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_170734 edited
  ATGCAGAGCCGGGAAGAGGAATGGTTCCACCAGGTGAGAAGAGTGATGACCATCCTTTTC
  CTTACTATGGTTATTTCATACTTTGGTTGCATGAAGGCTGCCCCCATGAAAGAAGCAAAC
  ATCCGAGGACAAGGTGGCTTGGCCTACCCAGGTGTGCGGACCCATGGGACTCTGGAGAGC
  GTGAATGGGCCCAAGGCAGGTTCAAGAGGCTTGACATCATTGGCTGACACTTTCGAACAC
  GTGATAGAAGAGCTGTTGGATGAGGACCAGAAAGTTCGGCCCAATGAAGAAAACAATAAG
  GACGCAGACTTGTACACGTCCAGGGTGATGCTCAGTAGTCAAGTGCCTTTGGAGCCTCCT
  CTTCTCTTTCTGCTGGAGGAATACAAAAATTACCTAGATGCTGCAAACATGTCCATGAGG
  GTCCGGCGCCACTCTGACCCTGCCCGCCGAGGGGAGCTGAGCGTGTGTGACAGTATTAGT
  GAGTGGGTAACGGCGGCAGACAAAAAGACTGCAGTGGACATGTCGGGCGGGACGGTCACA
  GTCCTTGAAAAGGTCCCTGTATCAAAAGGCCAACTGAAGCAATACTTCTACGAGACCAAG
  TGCAATCCCATGGGTTACACAAAAGAAGGCTGCAGGGGCATAGACAAAAGGCATTGGAAC
  TCCCAGTGCCGAACTACCCAGTCGTACGTGCGGGCCCTTACCATGGATAGCAAAAAGAGA
  ATTGGCTGGCGATTCATAAGGATAGACACTTCTTGTGTATGTACATTGACCATTAAAAGG
  GGAAGATAGTGGATTTATGTTGTATAGATTAGATTATATTGAGACAAAAATTATCTATTT
  GTATATATACATAACAGGGTAAATTATTCAGTTAAGAAAAAAATAATTTTATGAACTGCA
  TGTATAAATGAAGTTTATACAGTACAGTGGTTCTACAATCTATTTATTGGACATGTCCAT
  GACCAGAAGGGAAACAGTCATTTGCGCACAACTTAAAAAGTCTGCATTACATTCCTTGAT
  AATGTTGTGGTTTGTTGCCGTTGCCAAGAACTGAAAACATAAAAAGTTAAAAAAAATAAT
  AAATTGCATGCTGCTTTAATTGTGAATTGAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170734 unedited
  GTTCAGTTCACATTTGTATACGACTCATATAGGCGGCCGCGNATTCATGCAGAGCCGGGA
  AGAGGAATGGTTCCACCAGGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTAT
  TTCATACTTTGGTTGCATGAAGGCTGCCCCCATGAAAGAAGCAAACATCCGAGGACAAGG
  TGGCTTGGCCTACCCAGGTGTGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAA
  GGCAGGTTCAAGAGGCTTGACATCATTGGCTGACACTTTCGAACACGTGATAGAAGAGCT
  GTTGGATGAGGACCAGAAAGTTCGGCCCAATGAAGAAAACAATAAGGACGCAGACTTGTA
  CACGTCCAGGGTGATGCTCAGTAGTCAAGTGCCTTTGGAGCCTCCTCTTCTCTTTCTGCT
  GGAGGAATACAAAAATTACCTAGATGCTGCAAACATGTCCATGAGGGTCCGGCGCCACTC
  TGACCCTGCCCGCCGAGGGGAGCTGAGCGTGTGTGACAGTATTAGTGAGTGGGTAACGGC
  GGCAGACAAAAAGACTGCAGTGGACATGTCGGGCGGGACGGTCACAGTCCTTGAAAAGGT
  CCCTGTATCAAAAGGCCAACTGAAGCAATACTTCTACGAGACCAAGTGCAATCCCATGGG
  TTACACAAAAGAAAGCTGCAGGGGCATAGACAAAAGGCATTGGAACTCCCAGTGCCGAAC
  TACCCAGTCGTACGTGCGGGCCCTTACCATGGATAGCANNAAGAAATTGGCTGGCGATTC
  ATAAGGATAGACACTTCTTGTGTATGTACATTGACCATTAAAGGGGGAGATAGTGGNATT
  ATGTN
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_170734 unedited
  GATCAGTAGGATTGTGNNACCCTGTACTGTATAAACTTCATTTATACATGCAGTTCATAA
  AATATTTTTTTCTTAACTGAATAATTTACCCTGTTATGTATATATACAAATAGATAATTT
  TTGTCTCAATATAATCTAATCTATACAACATAAATCCACTATCTTCCCCTTTTAATGGTC
  AATGTACATACACAAGAAGTGTCTATCCTTATGAATCGCCAGCCAATTCTCTTTTTGCTA
  TCCATGGTAAGGGCCCGCACGTACGACTGGGTAGTTCGGCACTGGGAGTTCCAATGCCTT
  TTGTCTATGCCCCTGCAGCCTTCTTTTGTGTAACCCATGGGATTGCACTTGGTCTCGTAG
  AAGTATTGCTTCAGTTGGCCTTTTGATACAGGGACCTTTTCAAGGACTGTGACCGTCCCG
  CCCGACATGTCCACTGCAGTCTTTTTGTCTGCCGCCGTTACCCACTCACTAATACTGTCA
  CACACGCTCAGCTCCCCTCGGCGGGCAAGGTCAAAGTGGCGCCAGACCCCTCTGGACATG
  TTTGCAACATCTAAGAAATTTTTGAATCCCCCACCAAAAAAAAGAAAAGAAGCTCCAAAA
  GCACTTGACTACTGGACCACACCCTAGGACAGGTAAAAAGTCTGCCACCCTAATGGTTAT
  CTAACAAGGGACCCAAATTTTCTGGACCCCCAAACAAAAAACTCTTAGTATAAACGTTTA
  AAAAAAGGAGTAACCCCAATGGAGTCCAGACCTCATGGAAAACAGCCGTGGGGAGAAAAA
  TAAAGAAACACACAAAACACCAAAAGAGACCCCACAAACGTGGTTAGAGAAAAAAAACCC
  AAGGGACCGGAAAGGATACCCATAGTCTCAAGCGACACAAACCACAAAAGGCACACAAAA
  GAGAGGAGAACAAACAGTAGAAANAAGAGAAGATGTAGGAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_170734
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170734.2, NP_733930.1
• RefSeq Size: 3958 bp
• RefSeq ORF: 789 bp
• Locus ID: 627
• UniProt ID: P23560
• Cytogenetics: 11p14.1
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane
• Protein Pathways: Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (6), also known as VIIb, BDNF6B, or 4I-5, represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (c) contains a longer N-terminus and lacks a predicted signal peptide compared to isoform a.