CACNA1I (NM_021096) Human Untagged Clone

CAT#: SC304909

CACNA1I (untagged)-Human calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA1I
• Synonyms: ca(v)3.3; Cav3.3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021096 edited
  GCCACCATGGCTGAGAGCGCCTCCCCGCCCTCCTCATCTGCAGCAGCCCCAGCCGCTGAG
  CCAGGAGTCACCACGGAGCAGCCCGGACCCCGGAGCCCCCCATCCTCCCCGCCAGGCCTG
  GAGGAGCCTCTGGATGGAGCTGATCCTCATGTCCCACACCCAGACCTGGCGCCTATTGCC
  TTCTTCTGCCTGCGACAGACCACCAGCCCCCGGAACTGGTGCATCAAGATGGTGTGCAAC
  CCGTGGTTTGAATGTGTCAGCATGCTGGTGATCCTGCTGAACTGCGTGACACTTGGCATG
  TACCAGCCGTGCGACGACATGGACTGCCTGTCCGACCGCTGCAAGATCCTGCAGGTCTTT
  GATGACTTCATCTTTATCTTCTTTGCCATGGAGATGGTGCTCAAGATGGTGGCCCTGGGG
  ATTTTTGGCAAGAAGTGCTACCTCGGGGACACATGGAACCGCCTGGATTTCTTCATCGTC
  ATGGCAGGGATGGTCGAGTACTCCCTGGACCTTCAGAACATCAACCTGTCAGCCATCCGC
  ACCGTGCGCGTCCTGAGGCCCCTCAAAGCCATCAACCGCGTGCCCAGTATGCGGATCCTG
  GTGAACCTGCTCCTGGACACACTGCCCATGCTGGGGAATGTCCTGCTGCTCTGCTTCTTT
  GTCTTCTTCATCTTTGGCATCATAGGTGTGCAGCTCTGGGCGGGCCTGCTGCGTAACCGC
  TGCTTCCTGGAGGAGAACTTCACCATACAAGGGGATGTGGCCTTGCCCCCATACTACCAG
  CCGGAGGAGGATGATGAGATGCCCTTCATCTGCTCCCTGTCGGGCGACAATGGGATAATG
  GGCTGCCATGAGATCCCCCCGCTCAAGGAGCAGGGCCGTGAGTGCTGCCTGTCCAAGGAC
  GACGTCTACGACTTTGGGGCGGGGCGCCAGGACCTCAATGCCAGCGGCCTCTGTGTCAAC
  TGGAACCGTTACTACAATGTGTGCCGCACGGGCAGCGCCAACCCCCACAAGGGTGCCATC
  AACTTTGACAACATCGGTTATGCTTGGATTGTCATCTTCCAGGTGATCACTCTGGAAGGC
  TGGGTGGAGATCATGTACTACGTGATGGATGCTCACTCCTTCTACAACTTCATCTACTTC
  ATCCTGCTTATCATAGTGGGCTCCTTCTTCATGATCAACCTGTGCCTCGTTGTCATAGCG
  ACCCAGTTCTCGGAGACCAAGCAACGGGAGCACCGGCTGATGCTGGAGCAGCGGCAGCGC
  TACCTGTCCTCCAGCACGGTGGCCAGCTACGCCGAGCCTGGCGACTGCTACGAGGAGATC
  TTCCAGTATGTCTGCCACATCCTGCGCAAGGCCAAGCGCCGCGCCCTGGGCCTCTACCAG
  GCCCTGCAGAGCCGGCGCCAGGCCCTGGGCCCGGAGGCCCCGGCCCCCGCCAAACCTGGG
  CCCCACGCCAAGGAGCCCCGGCACTACCATGGGAAGACTAAGGGTCAGGGAGATGAAGGG
  AGACATCTCGGAAGCCGGCATTGCCAGACTTTGCATGGGCCTGCCTCCCCTGGAAATGAT
  CACTCGGGAAGAGAGCTGTGCCCGCAACATAGCCCCCTGGATGCGACGCCCCACACCCTG
  GTGCAGCCCATCCCCGCCACGCTGGCTTCCGATCCCGCCAGCTGCCCTTGCTGCCAGCAT
  GAGGACGGCCGGCGGCCCTCGGGCCTGGGCAGCACCGACTCGGGCCAGGAGGGCTCGGGC
  TCCGGGAGCTCCGCTGGTGGCGAGGACGAGGCGGATGGGGACGGGGCCCGGAGCAGCGAG
  GACGGAGCCTCCTCAGAACTGGGGAAGGAGGAGGAGGAGGAGGAGCAGGCGGATGGGGCG
  GTCTGGCTGTGCGGGGATGTGTGGCGGGAGACGCGAGCCAAGCTGCGCGGCATCGTGGAC
  AGCAAGTACTTCAACCGGGGCATCATGATGGCCATCCTGGTCAACACCGTCAGCATGGGC
  ATCGAGCACCACGAGCAGCCGGAGGAGCTGACCAACATCCTGGAGATCTGCAATGTGGTC
  TTCACCAGCATGTTTGCCCTGGAGATGATCCTGAAGCTGGCTGCATTTGGGCTCTTCGAC
  TACCTGCGTAACCCCTACAACATCTTCGACAGCATCATTGTCATCATCAGCATCTGGGAG
  ATTGTGGGGCAGGCGGACGGTGGGCTGTCGGTGCTGCGGACCTTCCGGCTGCTGCGCGTG
  CTGAAACTGGTGCGCTTCATGCCTGCCCTGCGGCGCCAGCTCGTGGTGCTCATGAAGACC
  ATGGACAACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCATC
  CTTGGGATGCATATTTTTGGCTGCAAGTTCAGCCTCCGCACGGACACTGGAGACACGGTG
  CCCGACAGGAAGAACTTCGACTCCCTGCTGTGGGCCATCGTCACTGTGTTCCAGATCCTC
  ACCCAGGAGGACTGGAACGTCGTTCTCTACAATGGCATGGCCTCCACTTCTCCCTGGGCC
  TCCCTCTACTTTGTCGCCCTCATGACCTTCGGCAACTATGTGCTCTTCAACCTGCTGGTG
  GCCATCCTGGTGGAGGGCTTCCAGGCGGAGGGTGACGCCAATCGCTCCTACTCGGACGAG
  GACCAGAGCTCATCCAACATAGAAGAGTTTGATAAGCTCCAGGAAGGCCTGGACAGCAGC
  GGAGATCCCAAGCTCTGCCCAATCCCCATGACCCCCAATGGGCACCTGGACCCCAGTCTC
  CCACTGGGTGGGCACCTAGGTCCTGCTGGGGCTGCGGGACCTGCCCCCCGACTCTCACTG
  CAGCCGGACCCCATGCTGGTGGCCCTGGGCTCCCGAAAGAGCAGTGTCATGTCTCTAGGG
  AGGATGAGCTATGACCAGCGCTCCCTGTCCAGCTCCCGGAGCTCCTACTACGGGCCATGG
  GGCCGCAGCGCGGCCTGGGCCAGCCGTCGCTCCAGCTGGAACAGCCTCAAGCACAAGCCG
  CCGTCGGCGGAGCATGAGTCCCTGCTCTCTGCGGAGCGCGGCGGCGGCGCCCGGGTCTGC
  GAGGTTGCCGCGGACGAGGGGCCGCCGCGGGCCGCACCCCTGCACACCCCACACGCCCAC
  CACATTCATCACGGGCCCCATCTGGCGCACCGCCACCGCCACCACCGCCGGACGCTGTCC
  CTCGACAACAGGGACTCGGTGGACCTGGCCGAGCTGGTGCCCGCGGTGGGCGCCCACCCC
  CGGGCCGCCTGGAGGGCGGCAGGCCCGGCCCCCGGGCATGAGGACTGCAATGGCAGGATG
  CCCAGCATCGCCAAAGACGTCTTCACCAAGATGGGCGACCGCGGGGATCGCGGGGAGGAT
  GAGGAGGAAATCGACTACACCCTGTGCTTCCGCGTCCGCAAGATGATCGACGTCTATAAG
  CCCGACTGGTGCGAGGTCCGCGAAGACTGGTCTGTCTACCTCTTCTCTCCCGAGAACAGG
  TTCCGGGTCCTGTGTCAGACCATTATTGCCCACAAACTCTTCGACTACGTCGTCCTGGCC
  TTCATCTTTCTCAACTGCATCACCATCGCCCTGGAGCGGCCTCAGATCGAGGCCGGCAGC
  ACCGAACGCATCTTTCTCACCGTGTCCAACTACATCTTCACGGCCATCTTCGTGGGCGAG
  ATGACATTGAAGGTAGTCTCGCTGGGCCTGTACTTCGGCGAGCAGGCGTACCTACGCAGC
  AGCTGGAACGTGCTGGATGGCTTTCTTGTCTTCGTGTCCATCATCGACATCGTGGTGTCC
  CTGGCCTCAGCCGGGGGAGCCAAGATCTTGGGGGTCCTCCGAGTCTTGCGGCTCCTGCGC
  ACCCTACGCCCCCTGCGTGTCATCAGCCGGGCGCCGGGCCTGAAGCTGGTGGTGGAGACA
  CTCATCTCCTCCCTCAAGCCCATCGGCAACATCGTGCTCATCTGCTGTGCCTTCTTCATC
  ATCTTTGGCATCCTGGGAGTGCAGCTCTTCAAGGGCAAGTTCTACCACTGTCTGGGCGTG
  GACACCCGCAACATCACCAACCGCTCGGACTGCATGGCCGCCAACTACCGCTGGGTCCAT
  CACAAATACAACTTCGACAACCTGGGCCAGGCTCTGATGTCCCTCTTTGTCCTGGCATCC
  AAGGATGGTTGGGTGAACATCATGTACAATGGACTGGATGCTGTTGCTGTGGACCAGCAG
  CCTGTGACCAACCACAACCCCTGGATGCTGCTGTACTTCATCTCCTTCCTGCTCATCGTC
  AGCTTCTTTGTGCTCAACATGTTTGTGGGTGTCGTGGTGGAGAACTTCCACAAGTGCCGG
  CAGCACCAGGAGGCTGAAGAGGCACGGCGGCGTGAGGAGAAGCGGCTGCGGCGCCTGGAG
  AAGAAGCGCCGGAAGGCCCAGCGGCTGCCCTACTATGCCACCTATTGTCACACCCGGCTG
  CTCATCCACTCCATGTGCACCAGCCACTACCTGGACATCTTCATCACCTTCATCATCTGC
  CTCAACGTGGTCACCATGTCCCTGGAGCACTACAATCAGCCCACGTCCCTGGAGACAGCC
  CTCAAGTACTGCAACTATATGTTCACCACTGTCTTTGTGCTGGAGGCTGTGCTGAAGCTG
  GTGGCATTTGGTCTGAGGCGCTTCTTCAAGGACCGATGGAACCAGCTGGACCTGGCCATT
  GTGCTACTGTCAGTCATGGGCATCACCCTGGAGGAGATCGAGATCAATGCGGCCCTGCCC
  ATCAATCCCACCATCATCCGCATCATGAGGGTTCTGCGCATTGCCCGAGTGCTGAAGCTG
  TTGAAGATGGCCACAGGAATGCGGGCCCTGCTGGACACGGTGGTGCAAGCTTTGCCCCAG
  GTGGGCAACCTGGGCCTCCTCTTCATGCTGCTCTTCTTCATCTATGCTGCTCTCGGGGTG
  GAGCTCTTTGGGAAGCTGGTCTGCAACGACGAGAACCCGTGCGAGGGCATGAGCCGGCAT
  GCCACCTTCGAGAACTTCGGCATGGCCTTCCTCACACTCTTCCAGGTCTCCACGGGTGAC
  AACTGGAACGGGATCATGAAGGACACGCTGCGGGACTGCACCCACGACGAGCGCAGCTGC
  CTGAGCAGCCTGCAGTTTGTGTCGCCGCTGTACTTCGTGAGCTTCGTGCTCACCGCGCAG
  TTCGTGCTCATCAACGTGGTGGTGGCTGTGCTCATGAAGCACCTGGACGACAGCAACAAG
  GAGGCGCAGGAGGACGCCGAGATGGATGCCGAGCTCGAGCTGGAGATGGCCCATGGCCTG
  GGCCCTGGCCCGAGGCTGCCTACCGGCTCCCCGGGCGCCCCTGGCCGAGGGCCGGGAGGG
  GCGGGCGGCGGGGGCGACACCGAGGGCGGCTTGTGCCGGCGCTGCTACTCGCCTGCCCAG
  GAGAACCTGTGGCTGGACAGCGTCTCTTTAATCATCAAGGACTCCTTGGAGGGGGAGCTG
  ACCATCATCGACAACCTGTCGGGCTCCATCTTCCACCACTACTCCTCGCCTGCCGGCTGC
  AAGAAGTGTCACCACGACAAGCAAGAGGTGCAGCTGGCTGAGACGGAGGCCTTCTCCCTG
  AACTCAGACAGGTCCTCGTCCATCCTGCTGGGTGACGACCTGAGTCTCGAGGACCCCACA
  GCCTGCCCACCTGGCCGCAAAGACAGCAAGGGTGAGCTGGACCCACCTGAGCCCATGCGT
  GTGGGAGACCTGGGCGAATGCTTCTTCCCCTTGTCCTCTACGGCCGTCTCGCCGGATCCA
  GAGAACTTCCTGTGTGAGATGGAGGAGATCCCATTCAACCCTGTCCGGTCCTGGCTGAAA
  CATGACAGCAGTCAAGCACCCCCAAGTCCCTTCTCCCCGGATGCCTCCAGCCCTCTCCTG
  CCCATGCCAGCCGAGTTCTTCCACCCTGCAGTGTCTGCCAGCCAGAAAGGCCCAGAAAAG
  GGCACTGGCACTGGAACCCTCCCCAAGATTGCGCTGCAGGGCTCCTGGGCATCTCTGCGG
  TCACCAAGGGTCAACTGTACCCTCCTCCGGCAGGCCACCGGGAGCGACACGTCGCTGGAC
  GCCAGCCCCAGCAGCTCCGCGGGCAGCCTGCAGACCACGCTCGAGGACAGCCTGACCCTG
  AGCGACAGCCCCCGGCGTGCCCTGGGGCCGCCCGCGCCTGCTCCAGGACCCCGGGCCGGC
  CTGTCCCCCGCCGCTCGCCGCCGCCTGAGCCTGCGCGGCCGGGGCCTCTTCAGCCTGCGG
  GGGCTGCGGGCGCATCAGCGCAGCCACAGCAGCGGGGGCTCCACCAGCCCGGGCTGCACC
  CACCACGACTCCATGGACCCCTCGGACGAGGAGGGCCGCGGTGGCGCGGGCGGCGGGGGC
  GCGGGCAGCGAGCACTCGGAGACCCTCAGCAGCCTCTCGCTCACCTCCCTCTTCTGCCCG
  CCGCCCCCGCCGCCAGCCCCCGGCCTCACGCCCGCCAGGAAGTTCAGCAGCACCAGCAGC
  CTGGCCGCCCCCGGCCGCCCCCACGCCGCCGCCCTGGCCCACGGCCTGGCCCGGAGCCCC
  TCGTGGGCCGCGGACCGCAGCAAGGACCCCCCCGGCCGGGCACCGCTGCCCATGGGCCTG
  GGCCCCTTGGCGCCCCCGCCGCAACCGCTCCCCGGAGAGCTGGAGCCGGGAGACGCCGCC
  AGCAAGAGGAAGAGATGA
  
• Restriction Sites: Please inquire
• ACCN: NM_021096
• Insert Size: 6700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021096.3, NP_066919.2
• RefSeq Size: 10007 bp
• RefSeq ORF: 6672 bp
• Locus ID: 8911
• UniProt ID: Q9P0X4
• Cytogenetics: 22q13.1
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway
• Gene Summary: This gene encodes the pore-forming alpha subunit of a voltage gated calcium channel. The encoded protein is a member of a subfamily of calcium channels referred to as is a low voltage-activated, T-type, calcium channel. The channel encoded by this protein is characterized by a slower activation and inactivation compared to other T-type calcium channels. This protein may be involved in calcium signaling in neurons. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).