NEDL2 (HECW2) (NM_020760) Human Untagged Clone

CAT#: SC304819

HECW2 (untagged)-Human HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDL2
• Synonyms: NDHSAL; NEDL2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020760 edited
  CAGACTGGGGCTGCAGATTTAGACGTAGACGTTTCCGAGGACGCGAGACTGCAGAGCTTT
  GTAGGCAGCCAATCCCAGAGAAGCAGACGGGATGGCTAGTTCAGCCCGGGAGCACCTGCT
  TTTTGTGAGGCGTCGAAATCCCCAGATGCGGTACACATTGAGCCCAGAGAACCTCCAGAG
  CCTTGCCGCCCAGAGCTCCATGCCAGAGAACATGACCCTGCAGCGGGCCAACAGCGACAC
  CGACCTGGTGACTTCTGAGAGCCGCTCCAGCTTAACTGCCAGCATGTACGAGTACACGCT
  GGGGCAAGCCCAGAACCTCATTATCTTCTGGGACATTAAAGAGGAGGTGGACCCCAGTGA
  TTGGATTGGACTTTATCATATAGATGAGAATTCTCCAGCCAACTTCTGGGATTCTAAGAA
  CAGGGGTGTGACTGGAACACAAAAAGGGCAAATTGTATGGAGAATTGAGCCTGGACCCTA
  TTTCATGGAACCGGAGATAAAAATCTGTTTTAAATATTACCACGGCATTAGTGGAGCCCT
  GCGAGCCACGACCCCCTGCATCACCGTGAAGAACCCAGCTGTGATGATGGGGGCAGAAGG
  CATGGAGGGAGGTGCTTCAGGAAACCTGCATTCTCGAAAACTTGTTAGCTTTACATTGTC
  AGATCTTAGGGCAGTTGGGCTAAAGAAAGGGATGTTCTTCAATCCTGACCCTTATCTTAA
  GATGTCAATTCAGCCAGGAAAGAAGAGCAGTTTCCCCACCTGTGCCCACCACGGGCAGGA
  GAGACGGTCTACTATCATCAGTAACACCACCAATCCAATTTGGCACCGAGAGAAATATTC
  CTTTTTTGCACTTCTTACTGATGTCTTAGAAATTGAAATTAAAGACAAATTTGCCAAGAG
  CCGTCCCATCATCAAGCGTTTTCTGGGGAAACTAACCATTCCAGTCCAGAGGCTGCTGGA
  GCGACAAGCCATCGGTGATCAAATGCTCAGCTACAACCTTGGCAGAAGGCTCCCAGCTGA
  CCACGTGAGTGGGTACCTCCAGTTTAAAGTGGAGGTTACGTCTTCTGTTCATGAAGATGC
  CTCTCCAGAAGCTGTTGGCACAATACTTGGAGTCAATTCTGTGAATGGAGACTTAGGTAG
  CCCTTCCGATGACGAGGACATGCCAGGGAGCCATCACGACAGCCAGGTGTGCTCTAATGG
  GCCAGTTTCTGAGGACAGTGCTGCCGATGGAACCCCCAAGCATTCATTCAGGACTAGCTC
  CACTCTGGAAATAGACACAGAGGAATTAACCTCTACGTCTTCAAGGACCTCACCTCCCAG
  AGGACGTCAGGATTCACTCAATGATTACTTAGATGCTATCGAACACAATGGCCACTCCAG
  GCCAGGGACAGCGACCTGCTCTGAGAGGTCCATGGGTGCCTCTCCGAAACTGAGGAGTAG
  TTTTCCCACTGACACAAGACTCAATGCCATGCTTCATATTGATTCAGATGAAGAAGATCA
  CGAGTTCCAGCAAGACCTGGGTTACCCATCTTCCTTGGAGGAGGAGGGAGGCCTGATCAT
  GTTTAGCAGGGCATCCAGAGCTGATGATGGAAGCCTGACATCTCAGACAAAGCTGGAGGA
  CAACCCTGTTGAGAATGAGGAAGCCTCCACACACGAAGCTGCTTCCTTTGAGGATAAGCC
  AGAAAATCTTCCAGAGCTTGCAGAGAGCTCCTTACCTGCAGGCCCAGCCCCAGAGGAAGG
  TGAAGGCGGCCCAGAGCCTCAACCCAGTGCTGACCAGGGCAGTGCTGAACTGTGTGGCTC
  TCAAGAGGTAGATCAGCCCACAAGTGGCGCAGACACAGGGACTTCCGATGCATCAGGAGG
  AAGCCGAAGAGCCGTCAGTGAGACCGAGTCTCTCGATCAGGGCTCTGAGCCTTCCCAGGT
  GTCCTCTGAAACAGAACCCAGTGATCCTGCCAGGACAGAGAGTGTGAGCGAAGCCAGCAC
  CAGGCCTGAGGGAGAGAGTGACCTGGAATGCGCTGACAGCTCCTGCAATGAGAGTGTGAC
  CACGCAGCTGTCCTCTGTGGACACACGGTGCTCCTCTCTTGAGAGCGCACGGTTTCCTGA
  AACCCCAGCCTTTTCTTCTCAGGAGGAGGAAGACGGAGCCTGTGCAGCAGAGCCCACCAG
  CAGTGGCCCTGCCGAAGGGTCGCAGGAATCCGTGTGCACTGCTGGTTCTTTACCTGTGGT
  ACAGGTGCCCAGTGGGGAGGATGAAGGGCCAGGGGCAGAATCGGCCACTGTACCTGACCA
  GGAGGAGCTGGGGGAGGTCTGGCAGCGGAGGGGGAGCCTGGAGGGAGCTGCAGCTGCTGC
  CGAGAGCCCACCGCAAGAAGAAGGCAGTGCTGGGGAGGCCCAAGGCACCTGTGAAGGGGC
  AACTGCCCAGGAGGAGGGCGCTACTGGAGGTTCTCAAGCCAACGGCCACCAGCCACTGCG
  ATCACTACCTTCAGTGCGCCAGGATGTTAGCCGGTACCAGAGGGTGGACGAGGCTCTCCC
  ACCAAACTGGGAGGCACGCATTGACAGCCACGGCAGGATCTTCTACGTGGATCACGTAAA
  CAGAACCACGACGTGGCAGCGACCGACAGCTCCCCCAGCCCCGCAGGTGCTGCAGAGATC
  TAACTCCATACAGCAAATGGAGCAGCTGAACCGGCGATATCAGAGTATCCGCAGAACCAT
  GACCAATGAGAGGCCTGAGGAAAATACCAATGCTATTGATGGGGCAGGGGAGGAAGCTGA
  CTTTCACCAAGCCAGTGCAGATTTCCGACGGGAGAACATCCTGCCTCACTCTACCTCCAG
  ATCCAGGATCACGTTGCTGTTACAGTCTCCCCCCGTGAAGTTCCTCATCAGCCCAGAGTT
  CTTCACCGTGCTGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACACGTGTTT
  GAAGCACATGATCACCAAAGTCCGGAGGGACACCCACCACTTTGAACGCTACCAGCATAA
  CCGCGACCTTGTGGGATTCCTCAACATGTTCGCGAACAAACAGCTAGAGCTGCCGCGGGG
  ATGGGAAATGAAACATGATCACCAGGGCAAGGCATTCTTTGTGGACCACAACTCCCGCAC
  CACCACTTTCATTGATCCCCGGCTCCCACTTCAGAGCAGTAGACCCACAAGTGCGCTGGT
  TCATCGGCAACACCTGACAAGGCAACGCAGCCACAGTGCGGGTGAGGTAGGAGAAGATTC
  TCGACATGCAGGACCACCAGTTCTTCCCAGGCCATCCAGTACATTCAATACAGTCAGTAG
  GCCACAGTACCAGGACATGGTTCCAGTGGCTTACAATGACAAGATTGTTGCATTTCTACG
  TCAACCCAATATCTTTGAAATTCTACAGGAGCGTCAACCTGATCTTACCAGGAATCACTC
  ACTCAGGGAGAAGATCCAATTTATCCGAACTGAAGGGACTCCAGGATTGGTGCGCCTTTC
  AAGCGATGCAGACCTTGTTATGTTGCTGAGCTTATTTGAAGAAGAGATAATGTCATATGT
  GCCTCCACATGCCTTACTCCACCCCAGCTACTGTCAGTCCCCACGTGGCTCTCCCGTGTC
  ATCTCCTCAGAACTCGCCAGGTACCCAGCGTGCCAATGCCCGGGCTCCAGCCCCTTACAA
  GCGGGATTTCGAAGCCAAACTGAGGAACTTTTACAGGAAGTTAGAGACTAAAGGATATGG
  ACAAGGCCCAGGGAAGTTAAAGTTAATTATCCGAAGAGATCACTTACTAGAAGATGCTTT
  TAATCAGATTATGGGCTACTCCAGAAAAGACCTGCAGAGAAATAAGCTATATGTCACCTT
  CGTTGGGGAGGAAGGGCTGGATTACAGTGGGCCTTCTAGAGAGTTTTTCTTCCTGGTATC
  CAGAGAACTCTTTAACCCATATTATGGCTTATTTGAATATTCAGCCAATGACACATACAC
  AGTACAAATAAGTCCTATGTCTGCTTTTGTAGACAATCACCATGAATGGTTCCGATTCAG
  TGGTAGGATCCTTGGTCTTGCACTAATACACCAGTATTTGTTGGATGCCTTCTTCACACG
  GCCCTTTTATAAGGCTCTTCTCAGAATTCTATGTGACCTGAGTGACCTAGAATACCTTGA
  TGAAGAGTTCCATCAGAGCCTGCAGTGGATGAAAGACAATGATATCCATGACATCCTAGA
  CCTCACGTTCACTGTGAACGAAGAAGTATTTGGGCAGATAACTGAACGAGAATTAAAGCC
  AGGGGGTGCCAATATCCCAGTTACAGAGAAGAACAAGAAGGAGTACATCGAGAGGATGGT
  GAAGTGGAGGATTGAGAGGGGTGTTGTACAGCAAACAGAGAGCTTAGTGCGTGGCTTCTA
  TGAGGTGGTGGATGCCAGGCTGGTATCTGTTTTTGATGCAAGAGAACTGGAATTGGTCAT
  CGCAGGCACAGCTGAAATAGACCTAAGTGATTGGAGAAACAACACAGAATATAGAGGAGG
  ATACCATGACAATCATATTGTAATTCGGTGGTTCTGGGCTGCAGTGGAAAGATTCAACAA
  TGAACAACGACTAAGGTTGTTACAGTTTGTTACAGGCACATCCAGCATTCCCTATGAAGG
  ATTTGCTTCACTCCGAGGGAGTAACGGCCCAAGAAGATTCTGTGTGGAGAAATGGGGGAA
  AATCACTGCTCTTCCCAGAGCGCATACATGTTTTAACCGTCTGGATCTGCCTCCCTACCC
  ATCCTTTTCCATGCTTTATGAAAAACTGTTGACAGCAGTTGAAGAAACCAGTACTTTTGG
  ACTTGAGTGACCTGGAAGCTGAATGCCCATCTCTGTGGACAGGCAGTTTCAGAAGCTGCC
  TTCTAGAAGAATGATTGAACATTGGAAGTTTCAAGAGGATGCTTCCTTTAGGATAAAGCT
  ACGTGCTGTTGTTTTCCAGGAACAAGTGCTCTGTCACATTTGGGGACTGGAGATGAGTCC
  TCTTGGAAGGATTTGGGTGAGCTTGATGCCCAGGGAACAACCCAACCGTCTTTCAATCAA
  CAGTTCTTGACTGCCAAACTTTTTCCATTTGTTATGTTCCAAGACAAAGATGAACCCATA
  CATGATCAGCTCCACGGTAATTTTTAGGGACTCAGGAGAATCTTGAAACTTACCCTTGAA
  CGTGGTTCAAGCCAAACTGGCAGCATTTGGCCCAATCTCCAAATTAGAGCAAGTTAAATA
  ATATAATAAAAGTAAATATATTTCCTGAAAGTACATTCATTTAAGCCCTAAGTTATAACA
  GAATATTCATTTCTTGCTTATGAGTGCCTGCATGGTGTGCACCATAGGTTTCCGCTTTCA
  TGGGACATGAGTGAAAATGAAACCAAGTCAATATGAGGTACCTTTACAGATTTGCAATAA
  GATGGTCTGTGACAATGTATATGCAAGTGGTATGTGTGTAATTATGGCTAAAGACAAACC
  ATTATTCAGTGAATTACTAATGACAGATTTTATGCTTTATAATGCATGAAAACAATTTTA
  AAATAACTAGCAATTAATCACAGCATATCAGGAAAAAGTACACAGTGAGTTCTGTTTATT
  TTTTGTAGGCTCATTATGTTTATGTTCTTTAAGATGTATATAAGAACCTACCTATCATGC
  TGTATGTATCACTCATTCCATTTTCATGTTCCATGCATACTCGGGCATCATGCTAATATG
  TATCCTTTTAAGCACTCTCAAGGAAACAAAAGGGCCTTTTATTTTTATAAAGGTAAAAAA
  AATTCCCCAAATATTTTGCACTGAATGTACCAAAGGTGAAGGGACATTACAATATGACTA
  ACAGCAACTCCATCACTTGAGAAGTATAATAGAAAATAGCTTCTAAATCAAACTTCCTTC
  ACAGTGCCGTGTCTACCACTACAAGGACTGTGCATCTAAGTAATAATTTTTTAAGATTCA
  CTATATGTGATAGTATGATATGCATTTATTTAAAATGCATTAGACTCTCTTCCATCCATC
  AAATACTTTACAGGATGGCATTTAATACAGATATTTCGTATTTCCCCCACTGCTTTTTAT
  TTGTACAGCATCATTAAACACTAAGCTCAGTTAAGGAGCCATCAGCAACACTGAAGAGAT
  CAGTAGTAAGAATTCCATTTTCCCTCATCAGTGAAGACACCACAAATTGAAACTCAGAAC
  TATATTTCTAAGCCTGCATTTTCACTGATGCATAATTTTCTTATTAATATTAAGAGACAG
  TTTTTCTATGGCATCTCCAAAACTGCATGACATCACTAGTCTTACTTCTGCTTAATTTTA
  TGAGAAGGTATTCTTCATTTTAATTGCTTTTGGGATTACTCCACATCTTTGTTTATTTCT
  TGACTAATCAGATTTTCAATAGAGTGAAGTTAAATTGGGGGTCATAAAAGCATTGGATTG
  ACATATGGTTTGCCAGCCTATGGGTTTACAGGCATTGCCCAAACATTTCTTTGAGATCTA
  TATTTATAAGCAGCCATGGAATTCCTATTATGGGATGTTGGCAATCTTACATTTTATAGA
  GGTCATATGCATAGTTTTCATAGGTGTTTTGTAAGAACTGATTGCTCTCCTGTGAGTTAA
  GCTATGTTTACTACTGGGACCCTCAAGAGGAATACCACTTATGTTACACTCCTGCACTAA
  AGGCACGTACTGCAGTGTGAAGAAATGTTCTGAAAAAGGGTTATAGAAATCTGGAAATAA
  GAAAGGAAGAGCTCTCTGTATTCTATAATTGGAAGAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_020760
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020760.1, NP_065811.1
• RefSeq Size: 6926 bp
• RefSeq ORF: 4719 bp
• Locus ID: 57520
• UniProt ID: Q9P2P5
• Cytogenetics: 2q32.3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of a family of E3 ubiquitin ligases which plays an important role in the proliferation, migration and differentiation of neural crest cells as a regulator of glial cell line-derived neurotrophic factor (GDNF)/Ret signaling. This gene also plays an important role in angiogenesis through stabilization of endothelial cell-to-cell junctions as a regulator of angiomotin-like 1 stability. The encoded protein contains an N-terminal calcium/lipid-binding (C2) domain involved in membrane targeting, two-four WW domains responsible for cellular localization and substrate recognition, and a C-terminal homologous with E6-associated protein C-terminus (HECT) catalytic domain. Naturally occurring mutations in this gene are associated with neurodevelopmental delay, hypotonia, and epilepsy. The decreased expression of this gene in the aganglionic colon is associated with Hirschsprung's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Both variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.