Capicua (CIC) (NM_015125) Human Untagged Clone

CAT#: SC304239

CIC (untagged)-Human capicua homolog (Drosophila) (CIC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Capicua
• Synonyms: MRD45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304239 representing NM_015125.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTATTCGGCCCACAGGCCCCTGATGCCCGCGTCCAGCGCGGCCTCCCGTGGCCTCGGCATGTTCGTG
  TGGACGAATGTGGAACCTCGCTCTGTGGCTGTGTTCCCTTGGCACTCCTTAGTCCCCTTCCTGGCACCC
  AGCCAGCCTGACCCCTCCGTGCAGCCGAGCGAGGCCCAGCAACCTGCCAGCCACCCAGTGGCCTCCAAC
  CAGAGCAAAGAACCTGCTGAGTCGGCAGCTGTTGCTCATGAACGGCCACCAGGTGGGACAGGGAGTGCT
  GACCCTGAGCGGCCCCCTGGAGCCACATGCCCTGAGAGCCCAGGACCCGGACCCCCACACCCTTTGGGG
  GTGGTGGAATCTGGTAAGGGTCCGCCTCCCACCACGGAGGAGGAGGCCTCCGGCCCCCCAGGAGAGCCC
  CGGCTGGACAGTGAGACAGAGAGTGACCATGATGATGCCTTCCTCTCCATCATGTCTCCTGAGATCCAG
  TTGCCTCTACCGCCCGGAAAACGTCGGACCCAGTCCCTCAGTGCCCTACCCAAGGAACGGGACTCATCT
  TCTGAGAAGGATGGACGCAGCCCCAACAAGCGGGAGAAGGACCACATCCGGCGGCCCATGAATGCCTTC
  ATGATCTTCAGCAAGCGGCACCGGGCCCTGGTCCACCAGCGTCATCCCAACCAGGACAACCGGACCGTC
  AGCAAGATCCTGGGCGAGTGGTGGTATGCCCTGGGGCCCAAGGAGAAGCAGAAGTACCACGACCTGGCC
  TTCCAGGTGAAGGAGGCCCACTTCAAGGCCCACCCAGATTGGAAGTGGTGCAACAAGGACCGAAAGAAG
  TCCAGCTCAGAGGCCAAGCCCACGAGCCTGGGGCTGGCAGGAGGGCACAAGGAGACGCGGGAGCGGAGC
  ATGTCGGAGACGGGCACTGCTGCTGCCCCTGGGGTGTCCTCTGAGCTCCTGTCCGTTGCAGCCCAGACA
  CTCCTGAGCTCAGACACCAAGGCTCCGGGGAGCAGCTCCTGTGGGGCAGAACGGCTACACACAGTTGGG
  GGACCTGGCTCAGCCCGGCCCCGAGCTTTCTCCCACAGCGGGGTACACAGCCTGGACGGCGGAGAAGTA
  GACAGTCAGGCGCTACAGGAACTGACGCAGATGGTGTCTGGCCCTGCATCGTACTCTGGCCCAAAGCCT
  TCTACCCAGTATGGAGCTCCAGGACCCTTTGCAGCCCCTGGTGAGGGAGGTGCCTTGGCGGCCACTGGG
  CGGCCCCCGCTGCTGCCCACCCGAGCTTCTCGTTCTCAGCGTGCGGCCAGTGAGGACATGACGAGTGAT
  GAGGAGCGCATGGTCATCTGTGAGGAGGAAGGGGATGATGATGTCATTGCTGACGATGGCTTCGGCACC
  ACTGACATTGATCTCAAGTGCAAGGAGCGGGTGACCGACAGCGAGAGTGGGGACAGCTCTGGGGAGGAC
  CCAGAGGGCAACAAGGGCTTTGGTCGGAAGGTGTTTTCACCTGTGATCCGTTCCTCCTTTACCCACTGC
  CGCCCCCCACTGGACCCTGAGCCCCCAGGGCCCCCGGATCCTCCTGTAGCCTTTGGCAAAGGCTATGGT
  TCCGCCCCATCCTCCTCTGCGTCCTCGCCTGCTTCCTCCTCAGCCTCGGCAGCCACCTCCTTCTCACTG
  GGCTCAGGAACCTTCAAGGCCCAGGAGTCTGGTCAGGGCAGCACAGCGGGCCCCCTACGGCCCCCACCC
  CCTGGGGCTGGGGGTCCAGCGACACCTTCCAAGGCAACCCGGTTCCTCCCAATGGATCCTGCCACCTTC
  CGGCGCAAGAGACCCGAAAGTGTGGGTGGCCTGGAGCCACCAGGCCCCTCAGTCATCGCGGCCCCTCCC
  AGCGGAGGAGGAAACATCCTGCAGACACTGGTGCTGCCCCCAAACAAGGAGGAGCAAGAGGGCGGCGGA
  GCCAGAGTGCCCTCCGCCCCCGCCCCATCACTGGCCTATGGGGCCCCAGCAGCTCCCCTGTCCCGTCCT
  GCCGCCACCATGGTCACCAATGTGGTGCGGCCTGTCAGCAGCACTCCTGTGCCCATCGCCTCTAAGCCC
  TTCCCCACCTCTGGCCGGGCTGAGGCGTCTCCAAATGACACAGCAGGTGCCAGGACTGAAATGGGCACT
  GGGTCTCGGGTGCCTGGGGGCTCCCCGCTGGGTGTCAGCTTAGTGTATTCGGACAAGAAGTCGGCAGCA
  GCCACCTCACCAGCCCCACACTTGGTGGCTGGACCCCTGCTGGGCACTGTGGGGAAGGCGCCTGCCACT
  GTCACTAACCTACTGGTGGGCACCCCGGGGTATGGGGCCCCTGCGCCCCCTGCTGTCCAGTTCATTGCC
  CAGGGGGCCCCTGGTGGTGGGACCACTGCGGGCTCAGGAGCAGGTGCTGGGAGTGGCCCCAATGGGCCA
  GTACCCCTGGGCATCCTGCAACCAGGTGCCCTGGGCAAGGCTGGGGGAATCACCCAGGTACAGTACATC
  CTGCCCACGCTGCCCCAGCAGCTTCAGGTGGCACCTGCCCCAGCACCAGCCCCTGGGACCAAGGCAGCG
  GCTCCCAGCGGCCCTGCACCCACCACCAGCATCCGTTTCACCCTCCCACCGGGCACTTCCACCAACGGC
  AAAGTCCTGGCTGCCACTGCACCCACTCCTGGCATCCCCATCCTGCAGTCTGTACCCTCCGCCCCACCC
  CCCAAAGCCCAGTCAGTTTCTCCCGTGCAGGCCCCGCCCCCGGGTGGCTCAGCCCAGCTGCTGCCTGGG
  AAGGTCCTAGTGCCTCTGGCCGCCCCTAGCATGTCAGTGCGGGGTGGAGGGGCCGGCCAGCCACTGCCA
  CTGGTGAGCCCGCCCTTCTCAGTACCTGTGCAGAATGGTGCCCAGCCCCCCAGCAAGATCATCCAGCTG
  ACCCCGGTGCCTGTGAGCACACCCAGCGGCCTGGTGCCGCCCCTGAGCCCAGCCACACTCCCTGGACCC
  ACCTCTCAGCCTCAGAAGGTCCTGTTGCCCTCCTCCACCAGAATCACCTATGTGCAGTCAGCGGGCGGG
  CACGCGCTGCCCCTGGGTACCAGCCCTGCGTCCAGCCAGGCTGGAACAGTCACCTCGTACGGGCCCACG
  AGCTCTGTAGCTCTAGGCTTCACCTCGCTGGGGCCCAGCGGCCCCGCCTTCGTGCAGCCCCTGCTCTCA
  GCAGGCCAAGCCCCACTGCTGGCTCCCGGTCAGGTGGGCGTGTCACCTGTGCCCAGTCCCCAGCTGCCG
  CCTGCCTGTGCAGCCCCCGGAGGTCCTGTCATAACAGCATTTTACTCTGGCAGCCCTGCACCCACCTCC
  TCAGCACCCCTGGCCCAGCCATCCCAGGCCCCCCCAAGCCTGGTCTACACTGTGGCCACCAGCACAACC
  CCACCTGCAGCCACCATTCTGCCCAAGGGCCCGCCAGCCCCTGCCACTGCCACCCCAGCCCCGACTAGC
  CCTTTCCCCAGCGCCACAGCAGGTTCCATGACCTACAGCTTAGTGGCCCCCAAGGCCCAGCGGCCCAGC
  CCGAAGGCCCCCCAGAAAGTGAAGGCAGCCATCGCCAGCATTCCCGTGGGGTCCTTTGAGGCAGGTGCC
  TCTGGGCGGCCTGGCCCTGCACCCCGGCAGCCTCTGGAGCCTGGCCCAGTCCGAGAGCCAACTGCCCCA
  GAGTCTGAGCTTGAGGGGCAGCCCACACCACCAGCCCCTCCACCCCTGCCAGAGACCTGGACTCCCACG
  GCCCGGAGCAGCCCCCCACTGCCCCCACCTGCTGAGGAGCGGACCAGCGCCAAGGGCCCTGAGACCATG
  GCCAGCAAATTCCCCAGCTCATCTTCAGACTGGCGCGTCCCTGGGCAGGGCCTGGAGAATCGTGGGGAG
  CCTCCCACTCCTCCCAGCCCGGCCCCAGCTCCAGCTGTAGCCCCTGGTGGCAGCAGCGAGAGCAGCAGT
  GGGCGGGCAGCCGGGGACACCCCGGAGCGCAAGGAGGCGGCTGGTACTGGCAAGAAGGTGAAGGTGCGG
  CCCCCGCCCCTGAAGAAGACCTTTGACTCTGTGGACAACAGGGTCCTGTCAGAAGTGGACTTCGAAGAG
  CGCTTTGCTGAGTTGCCTGAGTTTCGGCCTGAGGAGGTGCTGCCCTCCCCCACCCTGCAGTCTCTGGCC
  ACCTCACCCCGGGCCATCCTGGGCTCTTACCGCAAGAAGAGGAAGAACTCCACGGACCTGGATTCAGCA
  CCCGAGGACCCCACCTCGCCCAAGCGCAAGATGAGAAGACGCTCCAGCTGCAGCTCGGAGCCCAACACC
  CCCAAGAGTGCCAAGTGCGAGGGGGACATCTTCACCTTTGACCGTACAGGTACAGAAGCCGAGGACGTG
  CTTGGGGAGCTAGAGTATGACAAGGTGCCATACTCCTCCCTGCGGCGCACCCTGGACCAGCGCCGGGCC
  CTGGTCATGCAGCTCTTTCAGGACCATGGCTTCTTCCCGTCAGCCCAGGCCACAGCCGCCTTCCAGGCC
  CGCTATGCAGACATCTTTCCCTCCAAGGTTTGTCTGCAGTTGAAGATCCGTGAGGTGCGCCAGAAGATC
  ATGCAGGCTGCCACTCCCACGGAGCAGCCCCCTGGAGCTGAGGCTCCTCTCCCTGTACCGCCCCCCACT
  GGCACCGCTGCTGCCCCTGCCCCCACTCCCAGCCCCGCAGGGGGCCCTGACCCCACCTCACCCAGCTCG
  GACTCTGGCACGGCCCAGGCTGCCCCGCCACTGCCTCCACCCCCAGAGTCGGGGCCTGGACAGCCTGGC
  TGGGAGGGGGCTCCCCAGCCCTCCCCCCCACCCCCAGGTCCCTCCACAGCTGCCACAGGCAGGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015125
• Insert Size: 4827 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015125.4
• RefSeq Size: 5490 bp
• RefSeq ORF: 4827 bp
• Locus ID: 23152
• UniProt ID: Q96RK0
• Cytogenetics: 19q13.2
• MW: 163.8 kDa
• Gene Summary: The protein encoded by this gene is an ortholog of the Drosophila melanogaster capicua gene, and is a member of the high mobility group (HMG)-box superfamily of transcriptional repressors. This protein contains a conserved HMG domain that is involved in DNA binding and nuclear localization, and a conserved C-terminus. Studies suggest that the N-terminal region of this protein interacts with Atxn1 (GeneID:6310), to form a transcription repressor complex, and in vitro studies suggest that polyglutamine-expansion of ATXN1 may alter the repressor activity of this complex. Mutations in this gene have been associated with olidogdendrogliomas (PMID:21817013). In addition, translocation events resulting in gene fusions of this gene with both DUX4 (GeneID:100288687) and FOXO4 (GeneID:4303) have been associated with round cell sarcomas. There are multiple pseudogenes of this gene found on chromosomes 1, 4, 6, 7, 16, 20, and the Y chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (CIC-S, PMID:17190598) has a distinct N-terminus and is shorter than isoform CIC-L.