TOGARAM1 (NM_015091) Human Untagged Clone

CAT#: SC304228

FAM179B (untagged)-Human family with sequence similarity 179, member B (FAM179B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TOGARAM1
• Synonyms: FAM179B; KIAA0423
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304228 representing NM_015091.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCTGCCCCCTCCGCGCTGCTTCTGCTGCCGCCCTTTCCAGTCCTCTCTACCTATCGGCTCCAG
  AGCCGCAGTCGTCCTTCCGCCCCAGAGACCGATGATAGTCGAGTTGGGGGCATTATGAGAGGAGAGAAA
  AACTACTACTTCCGTGGAGCTGCGGGGGACCACGGTTCCTGCCCCACTACAACTTCGCCTCTGGCCTCG
  GCCCTCTTGATGCCCTCGGAGGCAGTCTCAAGCAGCTGGTCTGAGTCTGGAGGCGGTTTGTCAGGGGGA
  GATGAAGAGGACACTCGGCTCCTTCAACTCCTCCGCACTGCCCGGGATCCTTCTGAGGCCTTCCAGGCT
  TTGCAAGCTGCTTTGCCGCGGCGGGGCGGTCGACTTGGCTTCCCCCGACGCAAGGAAGCTTTGTATCGG
  GCACTGGGCCGAGTGCTTGTGGAAGGAGGTAGTGATGAGAAGCGGCTCTGCTTGCAACTTCTCTCGGAC
  GTTCTCCGGGGTCAGGGGGAGGCAGGCCAGCTTGAAGAGGCCTTTAGCTTAGCACTTTTGCCTCAACTA
  GTTGTCTCGTTACGGGAAGAGAATCCAGCCCTGCGGAAAGATGCGCTGCAGATCCTTCATATATGTCTG
  AAACGTAGTCCTGGAGAGGTGCTGAGAACGCTTATACAACAAGGACTGGAAAGTACCGATGCCCGACTT
  AGAGCTTCCACAGCACTACTGCTTCCCATCTTGCTTACTACTGAGGACTTGTTGCTTGGTCTGGATCTC
  ACCGAGGTGATAATATCCCTAGCCCGAAAGCTTGGTGATCAGGAGACAGAAGAAGAATCTGAGACAGCT
  TTCTCCGCACTTCAACAAATTGGGGAGCGACTTGGCCAAGACAGGTTTCAATCTTACATTTCTCGTCTG
  CCCTCTGCCCTGAGGAGACACTACAATCGCCGCCTGGAGTCCCAGTTTGGAAGTCAGGTTCCTTATTAT
  TTGGAACTTGAAGCCTCTGGATTTCCTGAAGATCCCCTTCCCTGTGCAGTGACTCTTTCCAACAGCAAT
  CTTAAATTTGGGATTATTCCTCAGGAGCTGCATTCACGATTATTGGATCAGGAAGACTATAAGAACCGG
  ACCCAGGCCGTCGAAGAACTAAAGCAGGTGCTGGGAAAATTTAACCCTAGTTCTACTCCTCATTCTAGT
  CTTGTTGGCTTCATTAGTTTGCTATATAATTTGTTAGACGATTCTAACTTCAAAGTGGTGCATGGCACA
  CTTGAAGTCCTGCATTTACTGGTTATTCGCCTTGGAGAGCAGGTACAGCAGTTCTTGGGACCAGTTATA
  GCAGCTTCTGTCAAAGTGCTGGCGGACAACAAGTTGGTGATCAAACAAGAATACATGAAAATCTTCCTC
  AAGCTAATGAAGGAAGTAGGACCTCAGCAGGTGCTTTGTTTACTCCTGGAACATCTCAAACATAAGCAT
  TCCAGAGTGAGAGAGGAGGTGGTGAACATTTGCATCTGCTCCCTGCTGACCTATCCTAGTGAGGATTTT
  GACTTGCCCAAACTGTCCTTTGATCTTGCCCCAGCTCTTGTAGATAGCAAACGCAGGGTACGCCAAGCA
  GCTTTAGAAGCTTTTGCCGTATTGGCATCATCAATGGGCTCAGGTAAAACCAGCATCCTTTTTAAAGCT
  GTGGATACAGTTGAACTGCAAGATAATGGAGATGGAGTGATGAATGCTGTGCAGGCCAGATTGGCTAGG
  AAAACCTTACCAAGGCTCACAGAGCAGGGATTTGTGGAATATGCAGTACTGATGCCATCTTCTGCCGGG
  GGTAGGTCAAACCATTTGGCACATGGAGCAGATACGGACTGGCTTTTGGCTGGTAACAGAACTCAGAGT
  GCACACTGTCACTGTGGTGACCACGTGAGGGATAGCATGCACATTTATGGATCTTACAGCCCAACTATC
  TGTACCCGAAGGGTATTAAGTGCAGGAAAAGGAAAAAATAAATTACCATGGGAAAATGAGCAACCTGGA
  ATCATGGGAGAAAACCAGACCTCCACTTCCAAGGATATAGAGCAGTTTTCAACATATGATTTCATCCCA
  TCTGCAAAATTAAAGCTTTCTCAAGGAATGCCAGTCAATGATGATTTATGTTTTAGCAGAAAAAGAGTA
  TCAAGAAACTTATTTCAGAATAGTCGGGATTTTAACCCAGATTGTCTTCCTTTATGTGCTGCTGGTACT
  ACTGGGACTCATCAAACAAATCTTTCTGGGAAATGTGCACAACTTGGATTTTCACAAATATGTGGTAAA
  ACTGGCAGTGTGGGTTCTGACTTACAATTCCTAGGGACAACTAGCAGTCATCAAGAAAAAGTGTATGCT
  AGCCTCAATTTTGGCAGTAAGACACAGCAAACATTTGGTAGTCAAACAGAGTGTACTTCCTCAAATGGT
  CAAAATCCAAGTCCAGGAGCTTACATCCTTCCATCCTATCCTGTCTCATCACCTCGAACTAGTCCAAAG
  CATACATCTCCTCTTATTATATCTCCAAAGAAGTCTCAAGATAATTCTGTTAATTTCTCAAATTCCTGG
  CCTCTTAAAAGCTTCGAAGGACTATCAAAGCCAAGTCCACAGAAGAAGCTTGTCAGCCAAAAATCGTCT
  GATCCTACGGGTAGAAATCATGGAGAAAATTCTCAAGAAAAACCTCCAGTTCAGCTTACACCTGCCTTG
  GTGAGATCGCCATCTTCCCGACGAGGTCTAAATGGGACAAAGCCTGTTCCTCCCATACCAAGGGGAATA
  AGCCTTTTGCCTGATAAAGCTGATTTAAGCACAGTGGGACACAAAAAGAAAGAGCCTGATGATATTTGG
  AAGTGTGAAAAAGATAGTCTTCCAATTGATCTTTCAGAATTAAATTTCAAGGATAAAGATTTGGATCAA
  GAAGAGATGCATAGCTCTCTTAGGTCCCTTCGTAATAGTGCAGCTAAGAAAAGAGCAAAACTGAGTGGC
  AGTACTTCAGATCTTGAAAGCCCTGATTCTGCAATGAAGCTCGACTTGACGATGGACTCCCCGTCTCTG
  TCTTCCTCACCAAACATCAATTCTTACAGTGAAAGTGGAGTTTACAGCCAAGAATCATTGACTTCTTCT
  CTGTCTACAACTCCCCAGGGGAAGAGAATAATGTCAGACATATTTCCAACATTTGGGTCAAAACCTTGT
  CCAACAAGACTTTCTTCTGCAAAGAAAAAAATTTCTCATATTGCTGAACAAAGCCCCAGTGCAGGGTCA
  TCATCAAATCCACAGCAAATTTCCAGTTTTGACTTCACAACCACAAAGGCTTTATCAGAAGACTCAGTA
  GTAGTTGTTGGAAAAGGCGTATTTGGAAGTTTAAGTTCAGCACCAGCAACCTGCAGCCAATCAGTGATA
  TCTTCTGTGGAAAATGGGGATACATTTTCAATTAAACAAAGTATTGAACCACCATCAGGGATTTATGGA
  AGATCAGTCCAGCAAAATATTTCATCATATCTTGATGTTGAGAATGAAAAAGATGCTAAAGTTTCTATT
  TCTAAATCTACTTATAACAAGATGAGACAAAAGAGAAAAGAAGAGAAAGAACTGTTTCACAATAAAGAT
  TGTGAAAAGAAGGAAAAAAATTCCTGGGAACGAATGAGACATACAGGAACTGAGAAAATGGCATCTGAA
  AGTGAAACACCTACTGGAGCTATTTCACAGTATAAAGAAAGGATGCCTTCTGTCACTCATAGTCCAGAA
  ATAATGGATCTGTCAGAACTACGACCATTCTCTAAACCAGAAATAGCACTGACAGAAGCCCTGAGGCTT
  TTGGCTGATGAGGATTGGGAGAAGAAAATTGAGGGACTGAATTTTATTAGATGCTTAGCTGCTTTTCAT
  TCTGAGATACTGAACACAAAGTTGCATGAAACAAATTTTGCAGTTGTTCAAGAGGTGAAAAATTTACGT
  TCTGGAGTTTCTCGTGCTGCTGTGGTCTGTTTAAGTGATCTTTTCACTTATTTGAAAAAGAGCATGGAT
  CAAGAGCTAGATACCACAGTAAAAGTTTTGTTGCACAAGGCTGGTGAATCAAATACATTTATAAGAGAA
  GATGTTGACAAAGCATTGAGAGCTATGGTTAATAATGTAACTCCTGCACGTGCAGTTGTTTCTCTTATC
  AATGGTGGACAAAGGTATTATGGTCGAAAGATGCTGTTCTTCATGATGTGTCATCCTAACTTTGAAAAA
  ATGCTTGAAAAGTATGTCCCATCTAAAGATTTGCCATATATTAAGGACTCTGTTAGAAACTTACAGCAA
  AAGGGTTTGGGGGAGATACCATTAGATACTCCTTCAGCAAAAGGAAGACGATCTCATACTGGCAGTGTT
  GGAAATACAAGATCATCATCTGTTTCTAGAGATGCTTTCAATTCAGCTGAAAGAGCTGTAACTGAAGTT
  CGTGAAGTCACCAGAAAATCAGTCCCTCGTAATTCCTTAGAAAGTGCTGAGTACCTTAAACTCATAACT
  GGCTTATTAAATGCAAAAGACTTTCGTGATCGTATTAATGGGATTAAGCAGCTTTTATCAGATACAGAA
  AATAATCAAGACCTTGTTGTTGGAAACATTGTGAAGATTTTTGATGCTTTTAAATCTCGACTTCATGAT
  TCTAATAGTAAAGTAAATCTGGTGGCTCTGGAAACAATGCACAAAATGATTCCTCTACTTAGAGACCAC
  TTATCTCCTATAATCAACATGCTAATTCCAGCAATAGTGGATAACAATCTGAATTCCAAGAATCCAGGC
  ATCTATGCGGCTGCTACAAATGTTGTTCAGGCACTGAGTCAGCATGTAGACAATTACTTACTTCTACAG
  CCATTTTGCACAAAAGCTCAGTTTTTAAATGGAAAAGCAAAACAGGACATGACGGAAAAGCTTGCTGAT
  ATTGTTACGGAACTTTATCAAAGGAAGCCGCATGCCACAGAGCAGAAAGTGTTGGTTGTTTTATGGCAT
  CTCTTAGGAAATATGACAAATAGTGGCTCTCTGCCTGGAGCTGGAGGAAATATACGAACAGCCACAGCT
  AAATTATCAAAAGCACTCTTTGCACAGATGGGTCAGAATCTGTTAAATCAGGCTGCATCTCAACCACCA
  CATATCAAAAAGAGTTTGGAGGAATTACTCGATATGACAATTTTAAATGAATTATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015091
• Insert Size: 5163 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015091.3
• RefSeq Size: 6280 bp
• RefSeq ORF: 5163 bp
• Locus ID: 23116
• UniProt ID: Q9Y4F4
• Cytogenetics: 14q21.2
• MW: 189.4 kDa
• Gene Summary: Required for normal structure and function of primary cilia. Plays a role in the organization of axoneme microtubule bundles in primary cilia (By similarity). Interacts with microtubules and promotes microtubule polymerization via its HEAT repeat domains, especially those in TOG region 2 and 4 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.