ARFGEF2 (NM_006420) Human Untagged Clone

CAT#: SC303772

ARFGEF2 (untagged)-Human ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (ARFGEF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARFGEF2
• Synonyms: BIG2; dJ1164I10.1; PVNH2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006420 edited
  ATGCAGGAGAGCCAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCC
  GACAAGGAGGTGAAGCGGCCCCAGCACTCCCAGCTGCGCAGGGCCTGCCAGGTGGCGCTC
  GATGAAATTAAAGCAGAAATAGAAAAGCAGAGGCTTGGCACTGCTGCACCACCAAAGGCA
  AACTTCATTGAAGCTGACAAGTATTTTCTTCCATTCGAGCTAGCTTGCCAGTCCAAGTCC
  CCAAGGGTAGTCAGCACATCCCTTGACTGCTTGCAGAAACTCATCGCATACGGGCACATC
  ACTGGCAACGCCCCTGACAGTGGAGCCCCTGGGAAGCGGCTGATCGACAGAATTGTTGAA
  ACCATTTGCAGTTGTTTTCAGGGCCCTCAGACTGATGAAGGGGTTCAGTTACAAATAATT
  AAGGCTCTTCTGACTGCAGTGACTTCCCCACACATTGAAATTCATGAGGGTACTATCCTG
  CAGACAGTGAGAACATGTTACAATATCTATTTGGCCAGCAAAAATCTCATCAATCAAACC
  ACTGCCAAGGCTACCCTTACTCAGATGCTGAACGTCATTTTCACCCGCATGGAAAACCAA
  GTGTTGCAGGAGGCCAGAGAACTGGAAAAACCAATCCAGTCAAAACCCCAGTCCCCTGTG
  ATCCAAGCTGCAGCAGTATCCCCAAAGTTCGTTCGTTTGAAGCACAGTCAGGCACAAAGC
  AAACCAACAACTCCCGAAAAAACAGATTTAACCAACGGTGAACATGCCAGGAGTGATTCT
  GGAAAAGTAAGCACAGAAAATGGAGACGCACCCAGAGAAAGAGGCTCATCACTGTCAGGG
  ACTGATGACGGAGCCCAGGAGGTGGTGAAGGACATCTTGGAAGATGTAGTCACATCTGCC
  ATTAAAGAAGCAGCGGAAAAGCATGGTCTGACAGAACCTGAGAGAGTTCTAGGTGAACTG
  GAGTGCCAGGAATGTGCTATTCCCCCAGGAGTTGATGAAAACTCACAGACCAACGGGATA
  GCCGATGACAGGCAGTCCTTGTCGTCAGCAGATAATCTGGAATCGGATGCACAAGGACAT
  CAAGTGGCTGCCAGGTTCTCCCACGTTCTGCAGAAGGATGCCTTCCTTGTGTTCCGCTCC
  CTGTGCAAGCTGTCCATGAAACCCCTTGGTGAAGGCCCTCCAGACCCAAAATCCCATGAG
  CTGCGTTCCAAGGTGGTTTCCCTGCAGCTGCTCCTCTCTGTGTTGCAAAATGCTGGCCCC
  GTATTCAGGACTCACGAGATGTTCATCAATGCAATCAAGCAATATCTCTGTGTGGCCTTG
  TCCAAAAACGGCGTCTCTTCAGTGCCTGATGTCTTTGAGCTCTCTCTTGCCATTTTTCTT
  ACTCTTCTTTCAAACTTTAAAATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAG
  ATTTTCCTGAACATTTTAGAAACATCAACAAGTTCTTTTGAGCACAGGTGGATGGTCATT
  CAGACTCTGACGAGGATCTGTGCAGATGCCCAGTGTGTTGTGGATATTTATGTCAACTAC
  GACTGTGATTTAAATGCTGCTAACATTTTTGAGCGCCTTGTAAATGATTTATCCAAAATT
  GCTCAGGGAAGAAGTGGACATGAGCTGGGAATGACACCTCTGCAGGAGCTCAGCCTGAGG
  AAGAAAGGCCTGGAGTGCCTCGTGTCCATTCTCAAGTGCATGGTGGAGTGGAGCAAAGAC
  CTGTATGTGAATCCCAACCACCAGACCAGCCTCGGTCAGGAGAGGCTCACGGATCAGGAA
  ATAGGGGATGGGAAAGGCCTTGACATGGCAAGACGGTGTAGTGTGACGTCCATGGAGTCC
  ACAGTGTCCTCGGGGACCCAGACAACTGTTCAGGATGACCCTGAGCAATTTGAGGTCATC
  AAGCAACAAAAAGAAATCATTGAACACGGCATCGAGCTGTTCAACAAGAAACCCAAGAGG
  GGGATCCAGTTTCTCCAGGAGCAGGGCATGCTGGGAACGTCAGTTGAAGACATAGCCCAA
  TTCCTGCACCAGGAGGAGCGCCTGGATTCCACCCAAGTAGGCGATTTTCTGGGAGATAGC
  GCAAGGTTCAACAAGGAGGTGATGTATGCCTACGTGGACCAACTTGACTTCTGTGAAAAA
  GAATTTGTCTCAGCCCTGCGGACATTCCTAGAAGGTTTCCGCCTACCTGGAGAAGCCCAA
  AAGATTGACCGATTAATGGAGAAGTTTGCCGCAAGATACATAGAATGCAACCAAGGGCAA
  ACTCTGTTTGCTAGTGCTGACACTGCTTATGTCCTAGCGTATTCAATTATTATGCTGACT
  ACAGACTTGCACAGTCCTCAGGTAAAAAATAAAATGACGAAAGAGCAGTATATTAAAATG
  AATCGGGGTATCAATGATAGTAAAGATCTGCCAGAAGAGTATCTCTCAAGCATCTATGAA
  GAGATAGAAGGCAAGAAAATTGCAATGAAAGAAACAAAAGAGCTAACGATTGCAACCAAA
  TCTACTAAGCAGAATGTAGCTAGTGAAAAGCAGCGGCGGCTGCTGTACAACTTAGAGATG
  GAGCAAATGGCTAAAACAGCCAAAGCTCTGATGGAGGCTGTGAGCCATGCCAAAGCCCCG
  TTTACCAGTGCCACTCACCTGGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCA
  CTATTGGCAGCCTACAGCATCGGACTCCAGAACTGTGATGACACTGAAGTGGCCTCCTTG
  TGTTTGGAAGGCATCCGATGTGCAATCCGAATCGCCTGCATCTTTGGAATGCAGCTGGAA
  CGAGATGCCTATGTTCAGGCTCTTGCTCGCTTCTCCCTACTCACAGCCAGCTCCAGCATC
  ACAGAAATGAAGCAGAAAAACATCGACACCATTAAGACGCTTATCACAGTGGCTCACACC
  GATGGCAACTACCTTGGGAATTCCTGGCATGAGATCTTGAAATGCATCAGCCAGCTGGAG
  CTCGCTCAGCTGATAGGAACCGGTGTGAAGACGCGCTACCTGTCTGGATCTGGGCGTGAA
  AGAGAAGGGAGCCTGAAGGGCCACACATTGGCAGGAGAAGAGTTCATGGGCCTTGGCCTC
  GGTAATTTGGTGAGTGGCGGAGTGGATAAAAGACAGATGGCCAGCTTCCAAGAATCGGTT
  GGTGAGACCAGCTCGCAGAGTGTGGTTGTAGCTGTGGACAGGATTTTTACTGGGTCTACC
  AGACTGGATGGAAATGCAATAGTTGACTTTGTCCGCTGGCTGTGTGCTGTGTCCATGGAT
  GAACTGGCTTCCCCCCACCATCCTCGCATGTTCAGCTTGCAGAAGATTGTGGAGATATCA
  TACTACAACATGAATCGGATCCGACTACAGTGGTCTCGAATATGGCATGTGATTGGAGAT
  CACTTCAATAAGGTTGGCTGCAACCCTAATGAAGATGTGGCTATCTTTGCTGTTGACTCA
  TTAAGGCAACTCTCCATGAAGTTTCTTGAGAAGGGTGAATTAGCCAACTTCCGTTTCCAG
  AAAGATTTTCTGAGGCCCTTTGAGCATATTATGAAGAAAAACAGGTCTCCCACCATCCGG
  GACATGGCGATCCGCTGCATTGCCCAGATGGTGAACTCCCAGGCGGCCAACATCCGCTCA
  GGTTGGAAGAACATCTTTGCCGTGTTCCACCAGGCAGCCTCTGATCATGATGGGAACATT
  GTGGAGCTGGCCTTCCAGACCACTTGCCACATTGTCACAACTATTTTCCAGCACCATTTT
  CCTGCAGCCATCGATTCCTTTCAGGATGCTGTGAAGTGCTTATCAGAGTTCGCCTGCAAC
  GCCGCTTTCCCTGACACGAGCATGGAAGCGATTCGGCTCATCCGCTTCTGTGGCAAATAC
  GTCTCTGAGAGGCCTCGGGTGCTACAAGAATACACAAGTGATGACATGAATGTAGCTCCT
  GGTGACAGAGTCTGGGTCCGAGGCTGGTTCCCCATCTTATTCGAACTCTCCTGCATCATT
  AATAGATGCAAGTTAGATGTACGAACAAGGGGACTCACAGTCATGTTTGAGATCATGAAG
  AGCTATGGCCACACCTTTGAAAAGCACTGGTGGCAGGACCTGTTCAGAATTGTGTTTCGG
  ATTTTTGACAATATGAAACTCCCTGAGCAACTGTCAGAGAAATCTGAGTGGATGACAACA
  ACCTGCAATCACGCACTTTATGCTATTTGTGATGTTTTTACCCAGTTTTATGAAGCTTTG
  AATGAAGTTCTTCTTTCTGATGTATTTGCACAATTGCAGTGGTGTGTCAAACAAGATAAT
  GAACAGTTGGCGCGATCAGGTACAAATTGCTTAGAAAACTTAGTAATATCCAATGGAGAG
  AAATTCAGTCCTGAAGTCTGGGATGAAACCTGCAACTGTATGTTGGATATTTTCAAAACA
  ACCATCCCACATGTTTTGCTGACATGGAGACCTGTAGGAATGGAGGAAGATTCATCAGAA
  AAGCATTTGGATGTGGATCTGGACCGCCAGTCTTTAAGCAGCATAGATAAAAATCCCTCT
  GAGAGGGGACAGAGCCAGCTCTCTAACCCAACAGATGACAGCTGGAAGGGTAGACCATAC
  GCAAATCAGAAACTGTTTGCCAGCCTCCTCATCAAGTGTGTGGTCCAGTTGGAATTGATA
  CAGACCATTGACAACATTGTGTTCTACCCTGCGACGAGCAAAAAGGAGGATGCAGAGCAC
  ATGGTTGCCGCCCAGCAAGACACGCTGGATGCAGATATCCACATAGAGACGGAGGATCAG
  GGCATGTATAAGTACATGTCTTCCCAGCACCTCTTCAAGCTGTTGGACTGTTTGCAGGAA
  TCCCATTCATTCTCAAAGGCCTTCAACTCCAATTACGAGCAGCGGACTGTCCTGTGGCGA
  GCAGGTTTTAAGGGCAAGTCTAAACCCAATCTTCTAAAACAAGAAACCAGCAGCCTGGCC
  TGTTGTTTGAGGATCCTGTTTCGAATGTATGTTGATGAGAACCGCAGGGATTCCTGGGAA
  GAAATACAGCAGAGACTTTTAACTGTTTGCAGTGAAGCTCTTGCCTATTTCATCACTGTG
  AATTCTGAGAGCCATCGGGAGGCCTGGACAAGTCTCTTGTTGTTACTTCTAACTAAAACC
  CTCAAAATAAATGATGAAAAGTTCAAAGCACATGCTTCAATGTACTACCCCTACTTGTGT
  GAAATTATGCAGTTTGACCTGATCCCTGAGCTCCGAGCAGTTCTGCGGAAGTTCTTCCTA
  CGGATAGGTGTTGTGTATAAGATATGGATACCAGAAGAGCCATCACAGGTACCAGCAGCA
  CTGTCACCAGTGTGGTAGCCCTGGCTGCCCAGGCCAGTGCTGCAGCTCTGCAGAATGTTC
  AGCATGCCATTTCTGACTGGCACATCTCGTGAAGTTTCATAGAAACAAGGAGTTGGCATC
  TTGGATCTCAGAATGGCCTGGAAACGGATGGCCTCTACGCTGTTCCATCACAGTCTCCAA
  CTAAGGCTTATGGTATTTCATTAAACTGTTGCATACCCAGTTAGCACAGTAGGTGGGGAG
  TCTGCTTCATTTCTATCATTCCATTTTTCTGATTAAACTGTCAAATCTGTCATTGCATAT
  GCCATCGTTTTCTAGCAAAATCCCATGATTGGCTATAAACGTTTTGTAAGAAGTCACTCT
  CCTTGAAAATACTGAACATAGCTGTATAGGTTTGTGATTATTAGAGAATATGTTAATAAA
  ACTTCTTGTAACGGCTAACTGCCACCTAAAATATGCTGGGTTTTCTGTTGTTTGAGTGTG
  TTAGAGAAATTTGAATGTTTTTGTCAGTTACGAGTCAGCCGTAATTAGATTAGTTTAAGG
  ACAGGTCAGGATTAGAGAAGAGTTGTTTTTGTGTTTTGTTAATGTCTGAGTGATTTTTAA
  AGTATTTTACAAAAAGATATTGAAAATTTGGTTGAAGGCAGAGTTTAGTAATTAAGTTAG
  AATTAAGAGTTTTGCGAGGTTAAAAAAATGTGCCTCGTGGATCTCCCTGTTTTAGTAACA
  TGGAGAGAAAAAGTCTACACGAAAAAGTGAACAATTTAATGAAGATGATTAGCCTTCCTT
  GAAATAAGTATTTGTGGATGGGTGTTAAATTAAAATTTCCAGAATACACTGTCCATCTCA
  CACACTCTGAAATCTAATATATGAAGTAGTAATGAAAATGAAGTAGTAATTTAACCAGAG
  TTCATTTATCCTTGAATAAACCTTTTTATTTTCACCTCAGAAAAGTGAGTGTACTGGCAG
  TTAGTGTCACTGCTTTGCACAGTCCCCATTAAAGGACCCTCCAGAGAGGGACAGTAACTG
  TGCATGAGAAGCCGCTCCCATAAGCCTCCTCAGCCAGATGTCATGGGTGGAACTGGAGCT
  GTGTCGGGGCCAGCACAGCTGAACTGTGACAATGGCAGGAGGTGGCATGTGCCCAGCACT
  TCCATTAATCTGAGCCTAGGAGTTGAATTCTTTGGCAAGGTTGGATTCTGAGGTCCTTAT
  TATGTTAATGATGGTGCAATACTCTCACCTGCAGTAGAACTGAGTTCTGCTGCAGCTTGT
  GTAAAAGTGGGCAGTACACAAGTACGTCCCAAAGCCTGTGAGCAGTATACGTGGATGCTC
  ACCCATGAGAAGGAGCACACACGCCTCATTCTCTGCCCTCACCCACTGCTCACCTAGAGC
  ATCGCTGAGCGTTAGACAAAGTGTTACACAGAATGATTAAAACTTTCAGACTTCTACCTA
  TGCTCTTTAGTCCTGTAAATTGGGTTGTATTGATGTCAACTCTGGTGCCTTAGAAGTTAG
  TAGTTTGGGAACCTATCTGTAAAATCAGATGTTTTTTCTTTGTAGAGAAGGATTTCTGGT
  GCTTTTGCTTACTAAGAGACCGATATTCTTAAGTTGTTTTCTTGTTTTAACAGCCTTGAG
  AAATGTTTGGTTTTGGCCAGCAGAATTCTTGTCTACTTTTTTCTTTCCCAAAAAGTGTTT
  TTTAATTTCTCTACCAAAGAAAAAATGAGCAGGTTTAGGTTTTTACATGACTTATATACA
  TTAGATAAAAGGAGCTGTATAATTTAGCAGGAAGGGACTATGGGAGAATACTTTACTGTG
  AGTGGAAAATGTTAGCACATTTGACTGGTTTGCCCTGGAATCCACTGCACCTTTACACTG
  CACCATGAAACCTACACTCCCTGGTATCATAGCGCGTCATCACCTCAACAAGTCAGTCGT
  CTCCATTGATATTTGTACAAAAGGTATACATGGGGAACACGTGTTCATTCATTAAGTCCA
  TCTTGCGTGCAGCTATATCCCTGATTGGTTATTTTTCCTTTCCTTCTGAGGTTCTCATGT
  CATTTTCTTCATCGGATGTGACTAAAATTTTTCTGGTGTCTTCTGCCCTCTCTTTAATTT
  TGCCTCTTGAGGGGTAGCAGATGTGTCAGTGCATTTTATTACTTGCTGAAACATTCAGGC
  TTACATTTCTTATTAGTTTAGTATTTTAAAAGATTTAATTTTCTGAATGAGGCATTTGAA
  TTGTACCAGCAATGGACTTTTAAAAAATTGGATGTAAAACCATTCAGGGTGATTTTTCTT
  GTCAGTGGACAGTGACGAACAGAGATTTGAAATCCCTTACCTCCAATAATAAGCCATTCA
  GCCTAAATTCATTTTTATGAATAAATCTCTTCTTTCTCATGGTAAATGTGGCTTGTGCCA
  CTCAAACACTAGTGAAAGGGTATGTACAACCGCAACATCAGGCCAGGACACCATTTATTT
  AACCAAGTAATGGAGGAGAGTGAAACATTTTCCAAGGCCTTATTTCTTTTTCAGAATGCT
  TTAAGTGTTGATTATGTGTGCTGGGTCTCTAGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006420 unedited
  GGGGGAAGGAGGGGCGACATTTGTATACCATCATATAGGCGGCCGCTGATTCGCCCTTAT
  GCAGAGAGCCGACCAAGACATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCCGACAA
  GGAGGTGAAGCGGCCCCAGCACTCCCAGCTGCGCAGGGCCTGCCAGGTGGCGCTCGATGA
  AATTAAAGCAGAAATAGAAAAGCAGAGGCTTGGCACTGCTGCACCACCAAAGGCAAACTT
  CATTGAAGCTGACAAGTATTTTCTTCCATTCGAGCTAGCTTGCCAGTCCAAGTCCCCAAG
  GGTAGTCAGCACATCCCTTGACTGCTTGCAGAAACTCATCGCATACGGGCACATCACTGG
  CAACGCCCCTGACAGTGGAGCCCCTGGGAAGCGGCTGATCGACAGAATTGTTGAAACCAT
  TTGCAGTTGTTTTCAGGGCCCTCAGACTGATGAAGGGGTTCAGTTACAAATAATTAAGGC
  TCTTCTGACTGCAGTGACTTCCCCACACATTGAAATTCATGAGGGTACTATCCTGCAGAC
  AGTGAGAACATGTTACAATATCTATTTGGCCAGCAAAAATCTCATCAATCAAACCACTGC
  CAAGGCTACCCTTACTCAGATGCTGAACGTCATTTTCACCCGCCTGGAAAACCAAGTGTT
  GCACGATGCCAGAGAACTGGAAAAACCAATCCAGTCAAAACCCCCATTCCCTGTGATCCA
  AGCTGCAACAGTATCCCCAAAGTTCGTTCGTTTGAAGCACAGTCAGGCCCAAAGCAAACC
  AACAACCTCCCGAAAAAACAGATTTAACCCACGGTGGAACATGCCCGGAGTTGATTCTGG
  GAAAAGTAAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_006420 unedited
  NAACACGNGCCTCTAGGAGATGGCACTTNCCAGGNCCAGNGAGAGCACTGGNGGNAGGGG
  TCACAGGGNATGCCACCCGGGNATCTGTTCAGAAACGCTATGACCGCGGCCGCAATCTAG
  AGACCCAGCACACATAATCAACACTTAAGCATTCTGAAAAAAAATAAGCCTTGGAAAAGT
  TTCACTCTCCTCCATTACTTGGTTAAATAAATGGTGTCCTGGCCTGATGTTGCGGTTGTA
  CATACCCTTTCACTAGTGTTTGAGTGGCACAAGCCACATTTACCATGAGAAAGAAGAGAT
  TTATTCATAAAAATGAATTTAGGCTGAATGGCTTATTATTGGAGGTAAGGGATTTCAAAT
  CTCTGTTCGTCACTGTCCACTGACAAGAAAAATCACCCTGAATGGTTTTACATCCAATTT
  TTTAAAAGTCCATTGCTGGTACAATTCAAATGCCTCATTCAGAAAATTAAATCTTTTAAA
  ATACTAAACTAATAAGAAATGTAAGCCTGAATGTTTCAGCAAGTAATAAAATGCACTGAC
  ACATCTGCTACCCCTCAAGAGGCAAAATTAAAGAGAGGGCAGAAGACACCAGANAAATTT
  TAGTCACATCCGATGAAGAAAATGACATGAGAACCTCAGAAGGAAAAGGAAAAATAACCA
  ATCAGGGATATAGCTGCACGCAAGATGGACTTAATGAATGAACACGTGTTCCCCATGTAT
  ACCTTTTGTACAAATATCAATGGAAACGACTGACTTGTTTGAGTGATGACGCGCTATGAT
  ACCAGGGAGTGTAGGTTTCATGGTGCAGTGTAAAAGTGCAATGGATTCCAGGGCAAACCA
  GTCAAATGTGCTAACATTTTCCCATCACAGTAAAGTTTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_006420
• Insert Size: 7900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There are 7 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 4 amino acid.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006420.1, NP_006411.1
• RefSeq Size: 5860 bp
• RefSeq ORF: 5358 bp
• Locus ID: 10564
• UniProt ID: Q9Y6D5
• Cytogenetics: 20q13.13
• Gene Summary: ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008]