valyl tRNA synthetase (VARS) (NM_006295) Human Untagged Clone

CAT#: SC303765

VARS (untagged)-Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: valyl tRNA synthetase
• Synonyms: G7A; NDMSCA; VARS; VARS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303765 representing NM_006295.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCACCCTCTACGTCTCCCCTCACCCAGATGCCTTCCCCAGCCTCCGAGCCCTCATAGCCGCTCGC
  TATGGGGAGGCTGGGGAGGGTCCCGGATGGGGAGGAGCCCACCCCCGCATCTGTCTCCAGCCACCCCCG
  ACTAGCAGGACTCCCTTTCCCCCACCCCGCCTGCCGGCCCTGGAGCAGGGGCCCGGTGGGCTCTGGGTG
  TGGGGGGCCACGGCTGTGGCCCAGCTGCTGTGGCCAGCAGGCCTGGGGGGCCCAGGGGGCAGCCGGGCG
  GCTGTCCTTGTCCAACAGTGGGTCAGTTACGCCGACACGGAGTTAATACCAGCTGCCTGTGGAGCAACG
  CTGCCGGCCCTGGGACTCCGAAGCTCGGCCCAGGACCCCCAGGCTGTGCTGGGGGCCCTGGGCAGGGCC
  CTGAGCCCCTTGGAGGAGTGGCTTCGGCTGCACACCTACTTGGCCGGGGAGGCCCCCACTCTGGCTGAC
  CTGGCGGCTGTCACAGCCTTGCTGCTGCCTTTCCGATACGTCCTAGACCCACCTGCCCGCCGGATCTGG
  AATAATGTGACTCGCTGGTTTGTCACGTGTGTCCGGCAGCCAGAATTCCGAGCCGTGCTAGGAGAAGTG
  GTTCTATACTCAGGAGCCAGGCCTCTCTCTCATCAGCCAGGCCCCGAGGCTCCTGCCCTCCCAAAGACA
  GCTGCTCAGCTCAAGAAAGAGGCAAAGAAACGGGAGAAGCTAGAGAAATTCCAACAGAAGCAGAAGATC
  CAACAGCAGCAGCCACCTCCAGGGGAGAAGAAACCAAAACCAGAGAAGAGGGAGAAACGGGATCCTGGG
  GTCATTACCTATGACCTCCCAACCCCACCCGGGGAAAAGAAAGATGTCAGTGGCCCCATGCCCGACTCC
  TACAGCCCTCGGTATGTGGAGGCTGCCTGGTACCCTTGGTGGGAGCAGCAGGGCTTCTTCAAGCCAGAG
  TATGGGCGTCCTAATGTGTCAGCAGCAAATCCCCGAGGTGTCTTCATGATGTGCATCCCACCCCCCAAT
  GTGACAGGCTCCCTGCACCTGGGCCATGCACTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCAC
  CGCATGCGTGGGGAGACCACCCTGTGGAACCCTGGCTGTGACCATGCAGGTATTGCCACCCAGGTGGTG
  GTGGAGAAGAAGCTATGGCGTGAGCAGGGACTGAGCCGGCACCAGCTGGGCCGCGAGGCCTTTCTACAG
  GAAGTCTGGAAGTGGAAGGAGGAGAAAGGTGACCGGATTTACCACCAGTTGAAGAAGCTTGGCAGCTCC
  TTGGACTGGGATCGAGCCTGTTTCACCATGGACCCTAAACTCTCAGCAGCTGTGACAGAGGCCTTTGTC
  CGGCTTCACGAGGAAGGCATCATCTATCGCAGTACCCGCCTTGTTAACTGGTCCTGCACCCTCAACTCC
  GCCATCTCTGACATTGAGGTGGATAAGAAGGAGCTGACAGGTCGCACCCTGCTCTCCGTGCCTGGCTAC
  AAGGAGAAGGTGGAGTTCGGGGTCCTCGTGTCCTTTGCCTATAAGGTCCAAGGCTCAGATAGCGACGAG
  GAGGTGGTGGTGGCAACAACTCGGATCGAGACAATGCTGGGAGATGTGGCTGTAGCTGTGCACCCCAAA
  GATACCAGATACCAGCACCTGAAGGGGAAGAACGTGATCCACCCATTCCTGTCTCGGAGCCTTCCCATT
  GTCTTCGATGAATTTGTGGACATGGACTTTGGCACAGGTGCTGTGAAGATCACCCCCGCACATGACCAA
  AATGACTATGAAGTTGGGCAGCGGCACGGGCTGGAGGCCATCAGCATCATGGACTCCCGGGGGGCCCTC
  ATCAATGTGCCTCCGCCTTTCCTGGGCCTGCCCAGGTTTGAGGCCAGGAAAGCGGTGCTGGTGGCGCTG
  AAGGAGCGGGGACTGTTCCGTGGCATTGAGGACAACCCCATGGTGGTGCCACTTTGCAACCGGTCGAAG
  GACGTGGTAGAGCCTCTGCTGCGGCCGCAGTGGTACGTTCGCTGCGGGGAGATGGCCCAGGCTGCCAGC
  GCCGCTGTGACTCGGGGTGACCTCCGCATCCTGCCTGAGGCCCATCAGCGCACATGGCATGCCTGGATG
  GACAACATCCGGGAGTGGTGCATTTCCAGGCAGCTGTGGTGGGGCCATCGCATCCCAGCCTACTTTGTC
  ACTGTCAGTGACCCAGCGGTGCCCCCTGGGGAGGACCCTGATGGGCGGTACTGGGTGAGTGGACGCAAT
  GAGGCGGAGGCCCGGGAGAAGGCAGCCAAGGAGTTCGGAGTGTCCCCTGACAAGATCAGTCTCCAGCAA
  GATGAGGATGTATTGGATACCTGGTTCTCCTCTGGCCTCTTCCCCTTATCCATTTTGGGCTGGCCCAAC
  CAGTCAGAAGACCTGAGTGTGTTCTACCCCGGGACACTGCTGGAGACCGGTCATGACATCCTCTTCTTC
  TGGGTGGCCCGGATGGTCATGCTGGGCCTGAAGCTCACGGGCAGGCTGCCCTTTAGAGAGGTCTACCTC
  CATGCCATCGTGCGAGATGCTCACGGCCGGAAGATGAGCAAGTCTCTAGGCAATGTCATCGATCCCCTG
  GACGTCATCTATGGAATCTCCCTGCAGGGCCTCCACAACCAGCTGCTGAACAGCAACCTGGATCCCAGC
  GAGGTGGAGAAGGCCAAAGAAGGGCAGAAAGCTGACTTCCCAGCGGGGATTCCTGAATGTGGCACCGAT
  GCTCTCCGGTTTGGATTATGTGCCTACATGTCCCAGGGTCGTGACATCAACCTGGATGTGAACCGGATA
  CTGGGTTACCGCCACTTCTGCAACAAGCTCTGGAATGCCACCAAGTTTGCCCTTCGTGGCCTTGGGAAG
  GGTTTTGTGCCCTCACCCACCTCCCAGCCCGGAGGCCATGAGAGCCTGGTGGACCGCTGGATCCGCAGC
  CGCCTGACAGAGGCTGTGAGGCTCAGCAATCAAGGCTTCCAGGCCTACGACTTCCCGGCCGTCACCACT
  GCCCAGTACAGCTTCTGGCTCTATGAGCTCTGTGATGTCTACTTGGAGTGCCTGAAACCTGTACTGAAT
  GGGGTGGACCAGGTGGCAGCTGAGTGTGCCCGCCAGACCCTGTACACTTGCCTGGACGTTGGCCTGCGG
  CTGCTCTCACCCTTCATGCCCTTCGTGACGGAGGAGCTGTTCCAGAGGCTGCCCCGGAGGATGCCGCAA
  GCTCCCCCTAGCCTCTGTGTTACCCCCTACCCGGAGCCCTCAGAGTGCTCCTGGAAGGACCCCGAGGCA
  GAAGCCGCCCTTGAGCTGGCGCTAAGCATCACGCGAGCCGTGCGCTCCCTGCGGGCCGACTACAACCTC
  ACCCGGATCCGGCCTGACTGTTTCCTGGAAGTGGCGGATGAGGCCACGGGCGCCCTGGCATCGGCGGTG
  TCGGGCTACGTGCAGGCCCTGGCCAGCGCAGGTGTGGTGGCTGTTCTGGCCCTGGGGGCTCCCGCCCCC
  CAGGGTTGCGCTGTGGCTCTGGCTTCTGATCGCTGCTCCATCCACCTGCAGCTTCAGGGGCTGGTGGAC
  CCTGCACGGGAGCTGGGCAAGCTGCAAGCCAAGCGAGTTGAGGCCCAGCGGCAGGCCCAGCGTCTGCGG
  GAACGCCGTGCTGCCTCGGGCTATCCTGTCAAGGTGCCGCTCGAAGTCCAGGAGGCAGATGAAGCCAAG
  CTCCAACAGACAGAAGCAGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTATTCCAGAAGATGCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006295
• Insert Size: 3795 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006295.2
• RefSeq Size: 4308 bp
• RefSeq ORF: 3795 bp
• Locus ID: 7407
• UniProt ID: P26640
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• MW: 140.5 kDa
• Gene Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]