Steroidogenic Factor 1 (NR5A1) (NM_004959) Human Untagged Clone

CAT#: SC303555

NR5A1 (untagged)-Human nuclear receptor subfamily 5, group A, member 1 (NR5A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Steroidogenic Factor 1
• Synonyms: AD4BP; ELP; FTZ1; FTZF1; hSF-1; POF7; SF-1; SF1; SPGF8; SRXX4; SRXY3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004959 edited
  GGAGGACGGACGGACAGGGCCAGCCTGCTGTCCGGCTGCCGCCCGCCGTGGTGTGAGGGG
  GTTTCTGCGCACCCACAGTCGCCACCGTCCCACCTGGGCTGCCGGAGCCTCCCCCTGGAC
  CCCTGGTGCCCACTGCCACCCTCATCCGGTGTGAGAGCGCTGCTTCCGCTTCGCGGACGC
  CGCGGGCATGGACTATTCGTACGACGAGGACCTGGACGAGCTGTGCCCCGTGTGCGGGGA
  CAAGGTGTCCGGCTACCACTACGGACTGCTCACGTGTGAGAGCTGCAAGGGCTTCTTCAA
  GCGCACGGTGCAGAACAACAAGCACTACACGTGCACCGAGAGCCAGAGCTGCAAGATCGA
  CAAGACGCAGCGCAAGCGCTGTCCCTTCTGCCGCTTCCAGAAATGCCTGACGGTGGGGAT
  GCGCCTGGAAGCCGTGCGCGCTGACCGTATGAGGGGTGGCCGGAACAAGTTTGGGCCGAT
  GTACAAGCGGGACCGGGCCCTGAAACAGCAGAAGAAGGCACAGATTCGGGCCAATGGCTT
  CAAGCTGGAGACAGGGCCCCCGATGGGGGTGCCCCCGCCGCCCCCTCCCGCACCGGACTA
  CGTGCTGCCTCCCAGCCTGCATGGGCCTGAGCCCAAGGGCCTGGCCGCCGGTCCACCTGC
  TGGGCCACTGGGCGACTTTGGGGCCCCAGCACTGCCCATGGCCGTGCCCGGTGCCCACGG
  GCCACTGGCTGGCTACCTCTACCCTGCCTTTCCTGGCCGTGCCATCAAGTCTGAGTACCC
  GGAGCCTTATGCCAGCCCCCCACAGCCTGGGCTGCCGTACGGCTACCCAGAGCCCTTCTC
  TGGAGGGCCCAACGTGCCTGAGCTCATCCTGCAGCTGCTGCAGCTGGAGCCGGATGAGGA
  CCAGGTGCGGGCCCGCATCTTGGGCTGCCTGCAGGAGCCCACCAAAAGCCGCCCCGACCA
  GCCGGCGGCCTTCGGCCTCCTGTGCAGAATGGCCGACCAGACCTTCATCTCCATCGTGGA
  CTGGGCACGCAGGTGCATGGTCTTCAAGGAGCTGGAGGTGGCCGACCAGATGACGCTGCT
  GCAGAACTGCTGGAGCGAGCTGCTGGTGTTCGACCACATCTACCGCCAGGTCCAGCACGG
  CAAGGAGGGCAGCATCCTGCTGGTCACCGGGCAGGAGGTGGAGCTGACCACAGTGGCCAC
  CCAGGCGGGCTCGCTGCTGCACAGCCTGGTGTTGCGGGCGCAGGAGCTGGTGCTGCAGCT
  GCTTGCGCTGCAGCTGGACCGGCAGGAGTTTGTCTGCCTCAAGTTCATCATCCTCTTCAG
  CCTGGATTTGAAGTTCCTGAATAACCACATCCTGGTGAAAGACGCTCAGGAGAAGGCCAA
  CGCCGCCCTGCTTGACTACACCCTGTGCCACTACCCGCACTGCGGGGACAAATTCCAGCA
  GCTGCTGCTGTGCCTGGTGGAGGTGCGGGCCCTGAGCATGCAGGCCAAGGAGTACCTGTA
  CCACAAGCACCTGGGCAACGAGATGCCCCGCAACAACCTGCTCATCGAAATGCTGCAAGC
  CAAGCAGACTTGAGCCTGGGCCGGGGGCGGGGCCGGGACTGGGGGCGGGACTGGGGGCGG
  GGCCTGGGCGGGGCCGCAGCCACACCGCTGGCTCTGCATGGTTCATTTTCTGATGCCCAC
  CGAGGAGCCCCAGCCCCGTCCCAGAGGCCGCTGCCCCTGAGTTCTGACACTGTGTGTTTG
  GGAAGTGGGTGAGGCTGGGCAGGGCCTGGCGGAGGTGGAGTGGCCACTGGCACTTGCCTG
  CTGCTTGGAGTGCCCCAAGGAGGTGGCTGTTAACCACCCGCCCCGCCCCCTCCCTGCTCC
  CAGCTCTCTCTCCTGGAGTCTGAAGCCTGCAGGTCCGGGGAGGAGGTTCGGGATTCCCTG
  GTGGGCCTCGACGTCCCTTGGATCAGAGGTCATCCCTTCCTCCTCTCCTGGAAACAGACA
  GGGAGAAGTTGAGCAGGTATCAACTAGGGGAGGAGAGAGGGTCTCCAGTGTTCCCCCCAT
  AGAGACCAGGAGGGAGAGCCTCTGTTTTGTAAACTAAGGATAACCGAGTTTGCTAAATTG
  AGAGGGGCTATTGGGCCCTAGAGGACACTAGGAGACTGGTTAGGACAAAAAGACCTTCTC
  CCTAGCCCTTCTACCCCACCTGACCTCTGCAAGAGGGGGCATTGATACATCATCGGGAAA
  AAACTTTGCTCCAGGCATCACTGATTCCCTCTCCCACCCAAGGAGAACGTTTGGTACAAT
  CGACATCCTAGCCCCACCCAGAGGTGGCCCTCCCAGGCTGGTATTTATCTGCAAGGTTGT
  AGTCAAGAGGTTTTTCTCCCCGCTTTTTGTTTTTAAGCTTCTAGACACTCCTTGAAATGT
  GTGTGTGATGGAGGGAAGGGGACAGATTTGAGGACTGAAGCTGGGGCTTGGGGATTGCCA
  CTAAGTACAGCTGATGGTTTCTCCCCGGACACTCGCCTACTAAGTACCCTTGGGGTGGTG
  CTGGGTCATTACTTCTGAGCCCCAGCCCCAATCCAGAGAAGCGCTGTTGCCCGCCCTCCA
  CCCACTAGGTGAACAGCAGGATGCCCTGTTGGGGGCTTCAGGTCTCTGTGGGTGGGAATG
  CAAGTGAACTTGGGAGGGGGCACGGGCCTGTAGATCAGGGATAGCGCTGTTGATCCCCTC
  TCTGTGGCTCCAACCCGTTGGGTCCCTTGCTGCAAACCCATGAAGCTGGCCCTCAGCTCC
  CTGACCCCCTGTCCTAGGTCATGAAGGACACTCTGCAGGGTGAAGCACCAGGGAGAGGCC
  TCGGCTGTCTCCTGTCCCCGGCGGGGTGCCTGCTGTCCGTCCCGCTTTCATGTTACTGTT
  GCAGCTTGTGCTGAGCCTGCCCAGTTGGAGGAGACTGGGCACCCCTGCCTCCTGCCTCCC
  GCCTCCCGCCACCCTGTCTCAGTACCTCCCCCCCCCGCCCCCTGAAACATGTGCCCCTGC
  CAAGGCCGGAGACCCACAGCCCTGAAACGAGAAGTGCCCTTAAGGATCACCCCAGCCCCC
  ACAGCCCTGGAATAAATTTCGCAATTAGTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004959 unedited
  NNGGTCCAGTTCAGATATTTGTATACGACTCATATAGGGCGGCCGCGAATTCCCGGATAG
  GAGGACGGACGGACAGGGCCAGCCTGCTGTCCGGCTGCCGCCCGCCGTGGTGTGAGGGGG
  TTTCTGCGCACCCACAGTCGCCACCGTCCCACCTGGGCTGCCGGAGCCTCCCCCTGGACC
  CCTGGTGCCCACTGCCACCCTCATCCGGTGTGAGAGCGCTGCTTCCGCTTCGCGGACGCC
  GCGGGCATGGACTATTCGTACGACGAGGACCTGGACGAGCTGTGCCCCGTGTGCGGGGAC
  AAGGTGTCCGGCTACCACTACGGACTGCTCACGTGTGAGAGCTGCAAGGGCTTCTTCAAG
  CGCACGGTGCAGAACAACAAGCACTACACGTGCACCGAGAGCCAGAGCTGCAAGATCGAC
  AAGACGCAGCGCAAGCGCTGTCCCTTCTGCCGCTTCCAGAAATGCCTGACGGTGGGGATG
  CGCCTGGAAGCCGTGCGCGCTGACCGTATGAGGGGTGGCCGGAACAAGTTTGGGCCGATG
  TACAAGCGGGACCGGGCCCTGAAACAGCAGAAGAAGGCACAGATTCGGGCCAATGGCTTC
  AAGCTGGAGACAGGGCCCCCGATGGGGGTGCCCCCGCCGCCCCCTCCCGCACCGGACTAC
  GTGCTGCCTCCCAGCCTGCATGGGCCTGAGCCCAAGGGCCTGGCCGCCGGTCCACCTGCT
  GGGCCACTGGGCGACTTTGGGGCCCCAGCACTGCCCATGGCCGTGCCCGGTGCCCACGGG
  CCACTGGCTGGCTACCTCTACCCCTGCCTTTCTGGCCGTGCCATCAAGTCTGAGTACCCG
  GAGCCTTATGCCAGCCCCCCACAGCCTGGGCTGCCGTACAGCTACCCAGACC
  
• Restriction Sites: Please inquire
• ACCN: NM_004959
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004959.3, NP_004950.2
• RefSeq Size: 3119 bp
• RefSeq ORF: 1386 bp
• Locus ID: 2516
• UniProt ID: Q13285
• Cytogenetics: 9q33.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]