DVL1 (NM_004421) Human Untagged Clone

CAT#: SC303481

DVL1 (untagged)-Human dishevelled, dsh homolog 1 (Drosophila) (DVL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DVL1
• Synonyms: DRS2; DVL; DVL1L1; DVL1P1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004421 edited
  ATGGCGGAGACCAAGATTATCTACCACATGGACGAGGAGGAGACGCCGTACCTGGTCAAG
  CTGCCCGTGGCCCCCGAGCGCGTCACGCTGGCCGACTTCAAGAACGTGCTCAGCAACCGG
  CCCGTGCACGCCTACAAATTCTTCTTTAAGTCCATGGACCAGGACTTCGGGGTGGTGAAG
  GAGGAGATCTTTGATGACAATGCCAAGCTTCCCTGCTTCAACGGCCGCGTGGTCTCCTGG
  CTGGTCCTGGCTGAGGGTGCTCACTCGGATGCGGGGTCCCAGGGCACGGACAGCCACACA
  GACCTGCCCCCGCCTCTTGAGCGGACAGGCGGCATCGGGGACTCCCGGCCCCCCTCCTTC
  CACCCGAATGTGGCCAGCAGCCGTGACGGGATGGACAACGAGACAGGCACGGAGTCCATG
  GTCAGTCACCGGCGGGAGCGTGCCCGACGCCGGAACCGCGAGGAGGCCGCCCGGACCAAT
  GGGCACCCAAGGGGAGACCGACGGCGGGATGTGGGGCTGCCCCCAGACAGCGCGTCCACC
  GCCCTCAGCAGCGAGCTTGAGTCCAGCAGCTTTGTGGACTCGGACGAGGATGGCAGCACG
  AGCAGGCTCAGCAGCTCCACGGAGCAGAGCACCTCATCCAGACTCATCCGGAAGCACAAA
  CGCCGGCGGAGGAAGCAGCGCCTTCGGCAGGCGGACCGGGCCTCCTCCTTCAGCAGCATA
  ACCGACTCCACCATGTCCCTCAACATCGTCACTGTCACGCTCAACATGGAAAGACATCAC
  TTTCTGGGCATCAGCATCGTGGGGCAGAGCAACGACCGTGGAGACGGCGGCATCTACATT
  GGCTCCATCATGAAGGGCGGGGCTGTGGCCGCTGACGGCCGCATCGAGCCCGGCGACATG
  TTGCTGCAGGTGAATGACGTGAACTTTGAGAACATGAGCAATGACGATGCCGTGCGGGTG
  CTGCGGGAGATCGTTTCCCAGACGGGGCCCATCAGCCTCACTGTGGCCAAGTGCTGGGAC
  CCAACGCCCCGAAGCTACTTCACCGTCCCACGGGCTGACCCGGTGCGGCCCATCGACCCC
  GCCGCCTGGCTGTCCCACACGGCGGCACTGACAGGAGCCCTGCCCCGCTACGAGCTGGAA
  GAGGCGCCGCTGACGGTGAAGAGTGACATGAGCGCCGTCGTCCGGGTCATGCAGCTGCCA
  GACTCGGGACTGGAGATCCGCGACCGCATGTGGCTCAAGATCACCATCGCCAATGCCGTC
  ATCGGGGCGGACGTGGTGGACTGGCTGTACACACACGTGGAGGGCTTCAAGGAGCGGCGG
  GAGGCCCGGAAGTACGCCAGCAGCTTGCTGAAGCACGGCTTCCTGCGGCACACGGTCAAC
  AAGATCACCTTCTCCGAGCAGTGCTACTACGTCTTCGGGGATCTCTGCAGCAATCTCGCC
  ACCCTGAACCTCAACAGTGGCTCCAGTGGGACTTCGGATCAGGACACGCTGGCCCCGCTG
  CCCCACCCGGCTGCCCCCTGGCCTCTGGGTCAGGGCTACCCCTACCAGTACCCGGGACCC
  CCACCCTGCTTCCCGCCTGCCTACCAGGACCCGGGCTTTAGCTATGGCAGCGGCAGCACC
  GGGAGTCAGCAGAGTGAAGGGAGCAAAAGCAGTGGGTCCACCCGGAGCAGCCGCCGGGCC
  CCGGGCCGTGAGAAGGAGCGTCGGGCGGCGGGAGCTGGGGGCAGTGGCAGTGAATCGGAT
  CACACGGCACCGAGTGGGGTGGGGAGCAGCTGGCGAGAGCGTCCGGCCGGCCAGCTCAGC
  CGTGGCAGCAGCCCACGCAGTCAGGCCTCGGCTACCGCCCCGGGGCTCCCCCCGCCCCAC
  CCCACGACCAAGGCCTATACAGTGGTGGGGGGGCCACCCGGGGGACCCCCTGTCCGGGAG
  CTGGCTGCCGTCCCCCCGGAATTGACAGGCAGCCGCCAGTCCTTCCAGAAGGCTATGGGG
  AACCCCTGCGAGTTCTTCGTGGACATCATGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004421 unedited
  CGTCTGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGCCCCGCG
  TCCGAGCCTCGGGCCGGGCCCTGAGCGCGCGGGCTCCGCGCCGCCGCCGCGCCATGGCGG
  AGACCAAGATTATCTACCACATGGACGAGGAGGAGACGCCGTACCTGGTCAAGCTGCCCG
  TGGCCCCCGAGCGCGTCACGCTGGCCGACTTCAAGAACGTGCTCAGCAACCGGCCCGTGC
  ACGCCTACAAATTCTTCTTTAAGTCCATGGACCAGGACTTCGGGGTGGTGAAGGAGGAGA
  TCTTTGATGACAATGCCAAGCTTCCCTGCTTCAACGGCCGCGTGGTCTCCTGGCTGGTCC
  TGGCTGAGGGTGCTCACTCGGATGCGGGGTCCCAGGGCACGGACAGCCACACAGACCTGC
  CCCCGCCTCTTGAGCGGACAGGCGGCATCGGGGACTCCCGGCCCCCCTCCTTCCACCCGA
  ATGTGGCCAGCAGCCGTGACGGGATGGACAACGAGACAGGCACGGAGTCCATGGTCAGTC
  ACCGGCGGGAGCGTGCCCGACGCCGGAACCGCGAGGAGGCCGCCCGGACCAATGGGCACC
  CAAGGGGAGACCGACGGCGGGATGTGGGGCTGCCCCCAGACAGCGCGTCCACCGCCCTCA
  GCAGCGAGCTTGAGTCCAGCAGCTTTGTGGNACTCGACGAGGATGGCAGCACGAGCAGGC
  TCAGCAGCNTCACGGAGCAGAGCACCTCATCCAGACTCATCCGGAAGCACAAACGCCCGG
  CGGNAGAAGCAGCGCCTTTTCGCAGGCGACCGGGCCTCCTNCTTCAGCAGCATAACCGAC
  TTCACCATGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004421 unedited
  NCGCCGCCAGTGGAGGCAATTCGAGCCAGGAAGCATGGGGAGGGTCAAGGGTGCCCCCGG
  GCTCGTTCAGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTAAAATTAAACGCTTTTTAGTGTTTAAAATAAGCAGCATTTTACACAGAAGCAGCTTC
  TTTGTTTAACCATCTAAACGCTGGGACTTTGATACAGTATCTACAGCACAGACACGTTGG
  GGGCCAGAGAAGCCAGGAAGGCCGCGATGTGTGCGCGCAGTGTGTGCACTCACCAAGGAC
  GGGCCACCTGACTGCCCATCTCCCCAAGACCTCCCTCCCTGTGGCAGCTGTGCACATCGG
  GGCCCCTTGACCCTGCGGGCCATGGTCCCTCCCTGCCCTGGCTGGGACACGGTGGGCAGG
  ATGTCCAGCCCTCTCTCGTCTTCCGGTCCCGTCCTCCACGTACTTTCAGACTGTTGCCGG
  ATGGGAGGAGAGAAGGTGCAGGCTGCTCCAAGGGGCAGCAGCAGGTGGGACAGATGACAG
  GGTCGCCTCCTCCCCCGAGTCACCGGCCAGGCAATAAATAAATAAATTAGATCCCTACTC
  CAGACAGGGGGCCTGTGCACCGCAGGGGGTTGCCCCGCGTGGACCACCCTGGGGGCCTGG
  GCACAGCTGTGCAGGAGGTGGGGGTCAGCCGAGAGCCCGAGGGGGTCTTCCTCATCCCAG
  GAGGGATCCCCACCAGACACAAGGGGTTTTGGGAGGTCCGAGGCTTCTCGCTGAGGGGCA
  GAGAGGGAGCGCCCCCAACACGGCTGCTCAGACACAGGTGCTGTCAGGAGCTGGAGCAGC
  CAGGCTGCCAGGGCAGATGGTGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004421
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004421.2, NP_004412.2
• RefSeq Size: 2941 bp
• RefSeq ORF: 2013 bp
• Locus ID: 1855
• UniProt ID: O14640
• Cytogenetics: 1p36.33
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.