Malignant fibrous histiocytoma (MFHAS1) (NM_004225) Human Untagged Clone

SKU SC303456

MFHAS1 (untagged)-Human malignant fibrous histiocytoma amplified sequence 1 (MFHAS1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Malignant fibrous histiocytoma
• Synonyms: LRRC65; MASL1; ROCO4
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004225 edited; ATGGCTGGGATGGACAGTGGCAACCTGAAGACCGCGAGGCTGTGGCGGGACGCCGCCCTG; CGTGCCAGGAAGCTGCGGAGCAACCTGCGCCAGCTCACGCTTACCGCCGCCGGGGCCTGC; CCCGGGGCCGGGGCCGACGCGCTCGAGTCCCCCGCCTCCCCCCAGCTCGTGCTGCCGGCC; AACCTCGGGGACATTGAGGCACTGAACCTGGGGAACAACGGCCTGGAGGAGGTACCCGAG; GGGCTGGGGTCGGCGCTGGGCAGCCTGCGCGTCCTGGTCCTGCGCAGGAACCGCTTCGCC; CGGCTGCCCCCGGCGGTGGCCGAGCTCGGCCACCACCTCACCGAGCTGGACGTGAGCCAC; AACCGGCTGACCGCCCTGGGCGCGGAGGTGGTGAGTGCTCTGAGGGAGCTGCGGAAGCTC; AACCTCAGCCACAACCAGCTGCCCGCCCTGCCCGCCCAGCTGGGCGCTCTCGCTCACCTG; GAGGAGCTGGATGTCAGCTTTAACCGGCTGGCGCACCTGCCTGACTCCCTCTCCTGCCTC; TCCCGCCTGCGCACCCTGGACGTGGATCACAACCAGCTCACTGCCTTCCCCCGGCAGCTG; CTGCAGCTGGTGGCCCTGGAGGAGCTGGACGTGTCCAGCAACCGGCTGCGGGGCCTGCCT; GAGGATATCAGTGCCCTGCGTGCCCTCAAGATCCTCTGGCTGAGTGGGGCCGAGCTTGGC; ACGCTGCCCGCCGGCTTCTGCGAGCTGGCCAGTTTGGAGAGCCTCATGCTAGACAACAAC; GGGCTGCAGGCTCTGCCCGCCCAGTTCAGCTGCCTGCAGCGGCTCAAAATGCTCAACCTC; TCCTCCAACCTCTTCGAGGAGTTCCCTGCCGCGCTGCTGCCCCTGGCTGGTCTGGAGGAG; CTCTACCTTAGTCGCAACCAGCTCACCTCGGTGCCATCCCTTATCTCGGGCCTGGGCCGG; CTTCTCACCTTGTGGCTGGATAATAACCGCATCCGCTACCTGCCGGACTCCATCGTGGAG; CTGACCGGCCTGGAGGAGCTCGTGCTGCAGGGGAACCAGATCGCGGTGCTGCCCGACCAC; TTTGGCCAGCTCTCCCGGGTGGGTTTGTGGAAGATCAAAGACAACCCACTGATCCAGCCC; CCCTACGAGGTCTGCATGAAGGGGATCCCCTACATCGCAGCCTACCAGAAGGAACTGGCT; CATTCCCAGCCGGCGGTGCAGCCCCGGCTCAAGCTGCTCCTGATGGGGCATAAGGCTGCA; GGAAAGACTTTGCTGCGCCACTGCCTCACCGAGGAGAGAGTGGAGGGATGCCCAGGAGGA; GGGGACAAGGAGAAGTGCTACCCACCGTCACCTCCCCCTGTGAGCAAGGGCATCGAGGTG; ACCAGCTGGACGGCCGATGCCTCCCGGGGCCTGCGGTTCATCGTGTATGACTTAGCTGGG; GATGAAAGTTATGAGGTGATCCAGCCCTTCTTCCTGTCCCCAGGGGCCCTATACGTGCTG; GTGGTCAACTTGGCCACCTATGAGCCTCGCCACTTTCCTACCACCGTGGGCTCCTTCTTG; CATCGGGTCGGGGCGAGAGTGCCCCACGCGGTGGTGTGCATCGTGGGCACCCACGCAGAC; CTGTGCGGAGAGCGTGAGCTGGAGGAGAAATGTCTGGACATTCACCGCCAGATCGCCCTG; CAGGAGAAGCACGACGCGGAGGGACTGAGCCGCTTGGCCAAGGTGGTGGACGAGGCACTG; GCCCGGGACTTCGAGCTGCGCTCTGCCAGCCCCCACGCAGCCTACTATGGCGTTTCGGAC; AAGAACCTTCGACGGCGCAAGGCCCATTTTCAATACCTGCTCAACCACCGGCTGCAGATC; CTCTCCCCCGTGTTGCCTGTTAGCTGCAGGGACCCGCGCCACTTACGACGCCTTCGGGAC; AAGTTGCTGTCAGTTGCTGAGCACCGAGAGATCTTCCCCAACTTACACAGAGTACTGCCT; CGATCCTGGCAGGTGCTGGAGGAACTGCATTTCCAGCCACCTCAGGCCCAGCGACTGTGG; CTAAGCTGGTGGGACTCGGCGCGCTTGGGCCTGCAGGCGGGTCTGACCGAGGACCGACTG; CAGAGTGCCCTCTCCTACCTGCATGAGAGCGGCAAGCTACTCTACTTTGAGGACAGTCCG; GCTCTCAAGGAGCACGTCTTCCACAACCTCACCCGCCTCATCGACATCCTCAATGTCTTC; TTCCAGAGGGATCCCTCTTTGCTGCTGCATAAGCTGCTCCTAGGGACCAGTGGAGAGGGC; AAGGCGGAGGGGGAAAGCTCCCCGCCCATGGCGCGGTCCACCCCCAGCCAGGAACTGCTC; CGGGCCACCCAGCTCCATCAGTATGTGGAGGGCTTTCTGTTGCATGGGCTCTTGCCAGCT; CATGTCATTCGGTTGCTGCTTAAGCCTCATGTCCAGGCCCAGCAGGACTTGCAGCTGTTG; CTGGAGCTGCTGGAGAAGATGGGACTCTGTTACTGCCTCAATAAACCCAAGGGCAAGCCT; TTGAATGGGTCCACAGCTTGGTACAAGTTCCCATGCTATGTGCAGAACGAGGTGCCCCAT; GCAGAAGCCTGGATTAATGGGACCAACCTAGCTGGGCAGTCTTTTGTGGCTGAGCAGTTG; CAGATTGAATATAGTTTTCCTTTTACTTTTCCACCTGGGTTGTTTGCACGCTACAGTGTC; CAGATCAACAGCCATGTGGTGCACAGGTCGGATGGTAAATTTCAGATCTTTGCCTATAGA; GGGAAAGTTCCTGTGGTTGTGAGTTACAGACCTGCCAGGGGAGTCCTGCAGCCAGACACC; CTGTCCATTGCTAGCCATGCATCATTACCAAATATATGGACCGCATGGCAAGCCATAACC; CCCTTGGTGGAGGAACTGAATGTCCTACTTCAGGAATGGCCTGGACTGCACTACACCGTG; CACATTCTCTGTTCTAAGTGCCTTAAGAGAGGATCGCCCAATCCACATGCTTTTCCAGGG; GAGTTGCTGAGTCAGCCCAGACCGGAAGGAGTGGCAGAGATCATTTGCCCCAAGAACGGC; AGCGAGCGAGTAAATGTTGCCTTGGTTTACCCACCCACGCCGACTGTGATCAGCCCCTGT; TCCAAGAAGAATGTTGGTGAAAAGCACAGAAACCAGTGA;
• Restriction Sites: Please inquire
• ACCN: NM_004225
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: ORF matches with reference except there are 2 SNPs in the ORF.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004225.1, NP_004216.1
• RefSeq Size: 3159 bp
• RefSeq ORF: 3159 bp
• Locus ID: 9258
• UniProt ID: Q9Y4C4
• Cytogenetics: 8p23.1
• Summary: Identified in a human 8p amplicon, this gene is a potential oncogene whose expression is enhanced in some malignant fibrous histiocytomas (MFH). The primary structure of its product includes an ATP/GTP-binding site, three leucine zipper domains, and a leucine-rich tandem repeat, which are structural or functional elements for interactions among proteins related to the cell cycle, and which suggest that overexpression might be oncogenic with respect to MFH. [provided by RefSeq, Jul 2008]