TCF3 / E2A (TCF3) (NM_003200) Human Untagged Clone

CAT#: SC303283

TCF3 (untagged)-Human transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF3 / E2A
• Synonyms: AGM8; bHLHb21; E2A; E47; ITF1; p75; TCF-3; VDIR
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003200 edited
  GAGAATGAACCAGCCGCAGAGGATGGCGCCTGTGGGCACAGACAAGGAGCTCAGTGACCT
  CCTGGACTTCAGCATGATGTTCCCGCTGCCTGTCACCAACGGGAAGGGCCGGCCCGCCTC
  CCTGGCCGGGGCGCAGTTCGGAGGTTCAGGTCTTGAGGACCGGCCCAGCTCAGGCTCCTG
  GGGCAGCGGCGACCAGAGCAGCTCCTCCTTTGACCCCAGCCGGACCTTCAGCGAGGGCAC
  CCACTTCACTGAGTCGCACAGCAGCCTCTCTTCATCCACATTCCTGGGACCGGGACTCGG
  AGGCAAGAGCGGTGAGCGGGGCGCCTATGCCTCCTTCGGGAGAGACGCAGGCGTGGGCGG
  CCTGACTCAGGCTGGCTTCCTGTCAGGCGAGCTGGCCCTCAACAGCCCCGGGCCCCTGTC
  CCCTTCGGGCATGAAGGGGACCTCCCAGTACTACCCCTCCTACTCCGGCAGCTCCCGGCG
  GAGAGCGGCAGACGGCAGCCTAGACACGCAGCCCAAGAAGGTCCGGAAGGTCCCGCCGGG
  TCTTCCATCCTCGGTGTACCCACCCAGCTCAGGTGAGGACTACGGCAGGGATGCCACCGC
  CTACCCGTCCGCCAAGACCCCCAGCAGCACCTATCCCGCCCCCTTCTACGTGGCAGATGG
  CAGCCTGCACCCCTCAGCCGAGCTCTGGAGTCCCCCGGGCCAGGCGGGCTTCGGGCCCAT
  GCTGGGTGGGGGCTCATCCCCGCTGCCCCTCCCGCCCGGTAGCGGCCCGGTGGGCAGCAG
  TGGAAGCAGCAGCACGTTTGGTGGCCTGCACCAGCACGAGCGTATGGGCTACCAGCTGCA
  TGGAGCAGAGGTGAACGGTGGGCTCCCATCTGCATCCTCCTTCTCCTCAGCCCCCGGAGC
  CACGTACGGCGGCGTCTCCAGCCACACGCCGCCTGTCAGCGGGGCCGACAGCCTCCTGGG
  CTCCCGAGGGACCACAGCTGGCAGCTCCGGGGATGCCCTCGGCAAAGCACTGGCCTCGAT
  CTACTCCCCGGGTCACTCAAGCAATAACTTCTCGTCCAGCCCTTCTACCCCCGTGGGCTC
  CCCCCAGGGCCTGGCAGGAACGTCACAGTGGCCTCGAGCAGGAGCCCCCGGTGCCTTATC
  GCCCAGCTACGACGGGGGTCTCCACGGCCTGCAGAGTAAGATAGAAGACCACCTGGACGA
  GGCCATCCACGTGCTCCGCAGCCACGCCGTGGGCACAGCCGGCGACATGCACACGCTGCT
  GCCTGGCCACGGGGCGCTGGCCTCAGGTTTCACCAGTCCCATGTCGCTGGGTGGGCGGCA
  CGCAGGCCTGGTTGGAGGCAGCCACCCCGAGGACGGCCTCGCAGGCAGCACCAGCCTCAT
  GCACAACCACGCGGCCCTCCCCAGCCAGCCAGGCACCCTCCCTGACCTGTCTCGGCCTCC
  CGACTCCTACAGTGGGCTAGGGCGAGCAGGTGCCACGGCGGCCGCCAGCGAGATCAAGCG
  GGAGGAGAAGGAGGACGAGGAGAACACGTCAGCGGCTGACCACTCGGAGGAGGAGAAGAA
  GGAGCTGAAGGCCCCCCGGGCCCGGACCAGCCCAGACGAGGACGAGGACGACCTTCTCCC
  CCCAGAGCAGAAGGCCGAGCGGGAGAAGGAGCGCCGGGTGGCCAATAACGCCCGGGAGCG
  GCTGCGGGTCCGTGACATCAACGAGGCCTTTAAGGAGCTGGGGCGCATGTGCCAACTGCA
  CCTCAACAGCGAGAAGCCCCAGACCAAACTGCTCATCCTGCACCAGGCTGTCTCGGTCAT
  CCTGAACTTGGAGCAGCAAGTGCGAGAGCGGAACCTGAATCCCAAAGCAGCCTGTTTGAA
  ACGGCGAGAAGAGGAAAAGGTGTCAGGTGTGGTTGGAGACCCCCAGATGGTGCTTTCAGC
  TCCCCACCCAGGCCTGAGCGAAGCCCACAACCCCGCCGGGCACATGTGAAAGGTATGCCT
  CCGTGGGACGAGCCACCCGCTTTCAGCCCTGTGCTCTGGCCCCAGAAGCCGGACTCGAGA
  CCCCGGGCTTCATCCACATCCACACCTCACACACCTGTTGTCAGCATCGAGCCAACACCA
  ACCTGACAAGGTTCGGAGTGATGGGGGCGGCCAAGGTGACACTGGGTCCAGGAGCTCCCT
  GGGGCCCTGGCCTACCACTCACTGGCCTCGCTCCCCCTGTCCCCGAATCTCAGCCACCGT
  GTCACTCTGTGACCTGTCCCATGGATCCTGAAACTGCATCTTGGCCCTGTTGCCTGGGCT
  GACAGGAGCATTTTTTTTTTTTCCAGTAAACAAAACCTGAAAGCAAGCAACAAAACATAC
  ACTTTGTCAGAGAAGAAAAAAATGCCTTAACTATAAAAAGCGGAGAAATGGAAACATATC
  ACTCAAGGGGGATGCTGTGGAAACCTGGCTTATTCTTCTAAAGCCACCAGCAAATTGTGC
  CTAAGCGAAATATTTTTTTTAAGGAAAATAAAAACATTAGTTACAAGATTTTTTTTTTTC
  TTAATGTAGATGAAAATTAGCAAGGATGCTGCCTTTGGTCTCTGGTTTTTTTAAGCTTTT
  TTTGCATATGTTTTGTAAGCAACAAATTTTTTTGTATAAAAGTCCCGTGTCTCTCGCTAA
  AAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003200 unedited
  TTCAAGTCACAATTGTATACGACTCATATAGGCGGCCGCGNAATCGCCCTTGAGAATGAA
  CCAGCCGCAGAGGAGGCGCCTGTGGGCACAGACAAGGAGCTCAGTGACCTCCTGGACTTC
  AGCATGATGTTCCCGCTGCCTGTCACCAACGGGAAGGGCCGGCCCGCCTCCCTGGCCGGG
  GCGCAGTTCGGAGGTTCAGGTCTTGAGGACCGGCCCAGCTCAGGCTCCTGGGGCAGCGGC
  GACCAGAGCAGCTCCTCCTTTGACCCCAGCCGGACCTTCAGCGAGGGCACCCACTTCACT
  GAGTCGCACAGCAGCCTCTCTTCATCCACATTCCTGGGACCGGGACTCGGAGGCAAGAGC
  GGTGAGCGGGGCGCCTATGCCTCCTTCGGGAGAGACGCAGGCGTGGGCGGCCTGACTCAG
  GCTGGCTTCCTGTCAGGCGAGCTGGCCCTCAACAGCCCCGGGCCCCTGTCCCCTTCGGGC
  ATGAAGGGGACCTCCCAGTACTACCCCTCCTACTCCGGCAGCTCCCGGCGGAGAGCGGCA
  GACGGCAGCCTAGACACGCAGCCCAAGAAGGTCCGGAAGGTCCCGCCGGGTCTTCCATCC
  TCGGTGTACCCACCCAGCTCAGGTGAGGACTACGGCAGGGATGCCACCGCCTACCCGTCC
  GCCAAGACCCCCAGCAGCACCTATCCCGCCCCCTTCTACGTGGCAGATGGCAGCCTGCAC
  CCCTCAGCCGAGCTCTGGAGTCCCCCGGGCCAAGCGGGCTTCGGGCCCATGCTGGGTGGG
  GGCTCAATCCCGCTGGCCCTCCCGCCCGGTACGGCCCCGGGGGGCAGCAGTGGAAGCCAC
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003200 unedited
  AGNAAAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGCTCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAGCGAGAGA
  CACGGGACTTTTATACAAAAAAATTTGTTGCTTACAAAACATATGCAAAAAAAGCTTAAA
  AAAACCAGAGACCAAAGGCAGCATCCTTGCTAATTTTCATCTACATTAAGAAAAAAAAAA
  ATCTTGTAACTAATGTTTTTATTTTCCTTAAAAAAAATATTTCGCTTAGGCACAATTTGC
  TGGTGGCTTTAGAAGAATAAGCCAGGTTTCCACAGCATCCCCCTTGAGTGATATGTTTCC
  ATTTCTCCGCTTTTTATAGTTAAGGCATTTTTTTCTTCTCTGACAAAGTGTATGTTTTGT
  TGCTTGCTTTCAGGTTTTGTTTACTGGAAAAAAAAAAAATGCTCCTGTCAGCCCAGGCAA
  CAGGGCCAAGATGCAGTTTCAGGATCCATGGGACAGGTCACAGAGTGACACGGTGGCTGA
  GATTCGGGGACAGGGGGAGCGAGGCCAGTGAGTGGTAGGCCAGGGCCCCAGGGAGCTCCT
  GGACCCAGTGTCACCTTGGCCGCCCCCATCACTCCGAACCTTGTCAGGTTGGTGTTGGCT
  CGATGCTGACAACAGGTGTGTGAGGTGTGGATGTGGATGAAGCCCGGGGTCTCGAGTGGC
  CGTTCTGGGGCCAGAGCCAGGGCTGAAAGCGGGTGGCTCGTCCCACGAGGCATACCTTTC
  AATGTGCCCGGCGGGGTTGTGGGCTTCGCTCAGCCTGGGTGGGGAGCTGAAAGCCCATCT
  GGGGGTCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_003200
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There are 3 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 2 amino acid.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003200.1, NP_003191.1
• RefSeq Size: 4396 bp
• RefSeq ORF: 1965 bp
• Locus ID: 6929
• UniProt ID: P15923
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Gene Summary: This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (E12). This isoform is also known as Pan-2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.