VEGFA (NM_001025368) Human Untagged Clone
VEGFA (untagged)-Human vascular endothelial growth factor A (VEGFA), transcript variant 4
|Product Name||VEGFA (NM_001025368) Human Untagged Clone|
|Synonyms||MGC70609; MVCD1; VEGF; VPF|
Fully Sequenced ORF
>OriGene ORF within SC302396 sequence for NM_001025368 edited (data generated by NextGen Sequencing)
Clone variation with respect to NM_001025368.2
|ORF Size||1116 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||The ORF of this clone has been fully sequenced and found to perfectly match with NM_001025368.1. This clone uses an alternative start codon CTG.|
|Product Components||The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.|
|Protein Families||Secreted Protein, Druggable Genome|
|Protein Pathways||Cytokine-cytokine receptor interaction, mTOR signaling pathway, VEGF signaling pathway, Focal adhesion, Pathways in cancer, Renal cell carcinoma, Pancreatic cancer, Bladder cancer|
|Gene Summary||This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015].
Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (d) represented in this RefSeq is derived from the 5'-most CUG start codon, and is shorter than isoform a. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. This transcript variant is supported by the full-length mRNA BC058855.1. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55012.1.
|The use of this cDNA Clones has been cited in the following citations:|
Polylysine-modified polyethylenimine (PEI-PLL) mediated VEGF gene delivery protects dopaminergic neurons in cell culture and in rat models of Parkinson's Disease (PD)
,Abid Sheikh, M;Saeed Malik, Y;Xing, Z;Guo, Z;Tian, H;Zhu, X;Chen, X;,
Acta Biomater 2016,PubMed ID 28025049
|cDNA Clone Resources|