MAP3K19 (NM_001018046) Human Untagged Clone

CAT#: SC302138

MAP3K19 (untagged)-Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP3K19
• Synonyms: RCK; YSK4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302138 representing NM_001018046.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTTCTATGCCAAAACCAGAAAGACATGCTGAGTCATTGCTTGACATTTGTCATGATACAAACTCT
  TCTCCAACTGATTTGATGACAGTTACCAAAAATCAAAACATCATCTTGCAAAGCATCAGCAGAAGTGAG
  GAGTTCGACCAAGATGGTGACTGCAGTCATTCCACACTGGTTAATGAAGAAGAAGATCCCAGTGGTGGT
  AGACAGGACTGGCAACCCAGGACAGAAGGTGTTGAGATCACTGTAACTTTTCCAAGAGATGTCAGTCCT
  CCCCAAGAAATGAGCCAAGAAGACTTAAAAGAAAAGAATCTGATAAACTCATCGCTTCAAGAATGGGCA
  CAAGCACATGCAGTTTCTCATCCAAATGAAATAGAAACGGTGGAGCTCAGGAAAAAGAAGCTGACCATG
  CGGCCCTTAGTTTTGCAAAAAGAGGAAAGTTCCAGGGAGCTCTGCAATGTGAACTTGGGCTTTTTGCTA
  CCAAGATCTTGTTTAGAACTGAACATTTCCAAGTCTGTAACCAGAGAAGATGCTCCTCATTTTCTGAAG
  GAGCAGCAAAGAAAATCTGAAGAGTTTTCGACCTCTCATATGAAGTACAGTGGCCGAAGCATCAAGAGG
  CATAGTAGTGGGCTCAGGATATATGACAGGGAGGAGAAATTTCTCATCTCAAATGAAAAGAAGATATTT
  TCTGAAAATAGTTTAAAGTCTGAAGAACCTATCCTATGGACCAAGGGTGAGATTCTTGGAAAGGGAGCC
  TACGGCACAGTATACTGTGGTCTCACTAGTCAAGGACAGCTAATAGCTGTAAAACAGGTGGCTTTGGAT
  ACCTCTAATAAATTAGCTGCTGAAAAGGAATACCGGAAACTACAGGAAGAAGTAGATTTGCTCAAAGCA
  CTGAAACATGTCAACATTGTGGCCTATTTGGGGACATGCTTGCAAGAGAACACTGTGAGCATTTTCATG
  GAGTTTGTTCCTGGTGGCTCAATCTCTAGTATTATAAACCGTTTTGGGCCATTGCCTGAGATGGTGTTC
  TGTAAATATACGAAACAAATACTTCAAGGTGTTGCTTATCTCCATGAGAACTGTGTGGTACATCGCGAT
  ATCAAAGGAAATAATGTTATGCTCATGCCAACTGGAATAATAAAGCTGATTGACTTTGGCTGTGCCAGG
  CGTTTGGCCTGGGCAGGTTTAAATGGCACCCACAGTGACATGCTTAAGTCCATGCATGGGACTCCATAT
  TGGATGGCCCCAGAAGTCATCAATGAGTCTGGCTATGGACGGAAATCAGATATCTGGAGCATTGGTTGT
  ACTGTGTTTGAGATGGCTACAGGGAAGCCTCCACTGGCTTCCATGGACAGGATGGCCGCCATGTTTTAC
  ATCGGAGCACACCGAGGGCTGATGCCTCCTTTACCAGACCACTTCTCAGAAAATGCAGCAGACTTTGTG
  CGCATGTGCCTGACCAGGGACCAGCATGAGCGACCTTCTGCTCTCCAGCTCCTGAAGCACTCCTTCTTG
  GAGAGAAGTCACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001018046
• Insert Size: 1533 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001018046.2
• RefSeq Size: 1933 bp
• RefSeq ORF: 1533 bp
• Locus ID: 80122
• UniProt ID: Q56UN5
• Cytogenetics: 2q21.3
• Protein Families: Druggable Genome, Protein Kinase
• MW: 57.7 kDa