IGFBP3 (NM_001013398) Human Untagged Clone

CAT#: SC301776

IGFBP3 (untagged)-Human insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGFBP3
• Synonyms: BP-53; IBP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001013398 edited
  TGGGCGCTGAGGATCAGCCGCTTCCTGCCTGGATTCCACAGCTTCGCGCCGTGTACTGTC
  GCCCCATCCCTGCGCGCCCAGCCTGCCAAGCAGCGTGCCCCGGTTGCAGGCGTCATGCAG
  CGGGCGCGACCCACGCTCTGGGCCGCTGCGCTGACTCTGCTGGTGCTGCTCCGCGGGCCG
  CCGGTGGCGCGGGCTGGCGCGAGCTCGGGGGGCTTGGGTCCCGTGGTGCGCTGCGAGCCG
  TGCGACGCGCGTGCACTGGCCCAGTGCGCGCCTCCGCCCGCCGTGTGCGCGGAGCTGGTG
  CGCGAGCCGGGCTGCGGCTGCTGCCTGACGTGCGCACTGAGCGAGGGCCAGCCGTGCGGC
  ATCTACACCGAGCGCTGTGGCTCCGGCCTTCGCTGCCAGCCGTCGCCCGACGAGGCGCGA
  CCGCTGCAGGCGCTGCTGGACGGCCGCGGGCTCTGCGTCAACGCTAGTGCCGTCAGCCGC
  CTGCGCGCCTACCTGCTGCCAGCGCCGCCAGCTCCAGGTGAGCCGCCCGCGCCAGGAAAT
  GCTAGTGAGTCGGAGGAAGACCGCAGCGCCGGCAGTGTGGAGAGCCCGTCCGTCTCCAGC
  ACGCACCGGGTGTCTGATCCCAAGTTCCACCCCCTCCATTCAAAGATAATCATCATCAAG
  AAAGGGCATGCTAAAGACAGCCAGCGCTACAAAGTTGACTACGAGTCTCAGAGCACAGAT
  ACCCAGAACTTCTCCTCCGAGTCCAAGCGGGAGACAGAATATGGTCCCTGCCGTAGAGAA
  ATGGAAGACACACTGAATCACCTGAAGTTCCTCAATGTGCTGAGTCCCAGGGGTGTACAC
  ATTCCCAACTGTGACAAGAAGGGATTTTATAAGAAAAAGCAGTGTCGCCCTTCCAAAGGC
  AGGAAGCGGGGCTTCTGCTGGTGTGTGGATAAGTATGGGCAGCCTCTCCCAGGCTACACC
  ACCAAGGGGAAGGAGGACGTGCACTGCTACAGCATGCAGAGCAAGTAGACGCCTGCCGCA
  AGGTTAATGTGGAGCTCAAATATGCCTTATTTTGCACAAAAGACTGCCAAGGACATGACC
  AGCAGCTGGCTACAGCCTCGATTTATATTTCTGTTTGTGGTGAACTGATTTTTTTTAAAC
  CAAAGTTTAGAAAGAGGTTTTTGAAATGCCTATGGTTTCTTTGAATGGTAAACTTGAGCA
  TCTTTTCACTTTCCAGTAGTCAGCAAAGAGCAGTTTGAATTTTCTTGTCGCTTCCTATCA
  AAATATTCAGAGACTCGAGCACAGCACCCAGACTTCATGCGCCCGTGGAATGCTCACCAC
  ATGTTGGTCGAAGCGGCCGACCACTGACTTTGTGACTTAGGCGGCTGTGTTGCCTATGTA
  GAGAACACGCTTCACCCCCACTCCCCGTACAGTGCGCACAGGCTTTATCGAGAATAGGAA
  AACCTTTAAACCCCGGTCATCCGGACATCCCAACGCATGCTCCTGGAGCTCACAGCCTTC
  TGTGGTGTCATTTCTGAAACAAGGGCGTGGATCCCTCAACCAAGAAGAATGTTTATGTCT
  TCAAGTGACCTGTACTGCTTGGGGACTATTGGAGAAAATAAGGTGGAGTCCTACTTGTTT
  AAAAAATATGTATCTAAGAATGTTCTAGGGCACTCTGGGAACCTATAAAGGCAGGTATTT
  CGGGCCCTCCTCTTCAGGAATCTTCCTGAAGACATGGCCCAGTCGAAGGCCCAGGATGGC
  TTTTGCTGCGGCCCCGTGGGGTAGGAGGGACAGAGAGACAGGGAGAGTCAGCCTCCACAT
  TCAGAGGCATCACAAGTAATGGCACAATTCTTCGGATGACTGCAGAAAATAGTGTTTTGT
  AGTTCAACAACTCAAGACGAAGCTTATTTCTGAGGATAAGCTCTTTAAAGGCAAAGCTTT
  ATTTTCATCTCTCATCTTTTGTCCTCCTTAGCACAATGTAAAAAAGAATAGTAATATCAG
  AACAGGAAGGAGGAATGGCTTGCTGGGGAGCCCATCCAGGACACTGGGAGCACATAGAG:
  ATTCACCCATGTTTGTTGAACTTAGAGTCATTCTCATGCTTTTCTTTATAATTCACACAT
  ATATGCAGAGAAGATATGTTCTTGTTAACA:TTGTATACAACATAGCCCCAAATATAGTA
  AGATCTATACTAGATAATCCTAGATGAAATGTTAGAGATGCTATATGATACAACTGTGGC
  CATGACTGAGGAAAGGAGCTCACGCCCAGAGACTGGGCTGCTCTCCCGGAGGCCAAACCC
  AAGAAGGTCTGGCAAAGTCAGGCTCAGGGAGACTCTGCCCTGCTGCAGACCTCGGTGTGG
  ACACACGCTGCATAGAGCTCTCCTTGAAAACAGAGGGGTCTCAAGACATTCTGCCTACCT
  ATTAGCTTTTCTTTATTTTTTTAACTTTTTGGGGGGAAAAGTATTTTTGAGAAGTTTGTC
  TTGCAATGTATTTATAAATAGTAAATAAAGTTTTTACCATTAAAAAAATATCTTTCCCTT
  TGTTATTGACCATCTCTGGGCTTTGTATCACTAATTATTTTATTTTATTATATAATAATT
  ATTTTATTATAATAAAATCCTGAAAGGGGAAAATAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001013398 unedited
  NNAAGTTCACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGTGGGCG
  CTGAGGATCAGCCGCTTCCTGCCTGGATTCCACAGCTTCGCGCCGTGTACTGTCGCCCCA
  TCCCTGCGCGCCCAGCCTGCCAAGCAGCGTGCCCCGGTTGCAGGCGTCATGCAGCGGGCG
  CGACCCACGCTCTGGGCCGCTGCGCTGACTCTGCTGGTGCTGCTCCGCGGGCCGCCGGTG
  GCGCGGGCTGGCGCGAGCTCGGGGGGCTTGGGTCCCGTGGTGCGCTGCGAGCCGTGCGAC
  GCGCGTGCACTGGCCCAGTGCGCGCCTCCGCCCGCCGTGTGCGCGGAGCTGGTGCGCGAG
  CCGGGCTGCGGCTGCTGCCTGACGTGCGCACTGAGCGAGGGCCAGCCGTGCGGCATCTAC
  ACCGAGCGCTGTGGCTCCGGCCTTCGCTGCCAGCCGTCGCCCGACGAGGCGCGACCGCTG
  CAGGCGCTGCTGGACGGCCGCGGGCTCTGCGTCAACGCTAGTGCCGTCAGCCGCCTGCGC
  GCCTACCTGCTGCCAGCGCCGCCAGCTCCAGGTGAGCCGCCCGCGCCAGGAAATGCTAGT
  GAGTCGGAGGAAGACCGCAGCGCCGGCAGTGTGGAGAGCCCGTCCGTCTCCAGCACGCAC
  CGGGTGTCTGATCCCAAGTTCCACCCCCTCCATTCAAAGATAATCATCATCAAGAAAGGG
  CATGCTAAAGACAGCCAGCGCTACAAAGTTGACTACGAGTCTCAGAGCACAGATACCCAG
  AACTTCTCCTCCGAGTCCAAGCGGGAGACAGAATATGGTCCCCTGCGTAGAGAAATGGAA
  GAACACTGAATCACCTGAAGTTCCTCATGTGCTGAGTCCCAGGGTGTACACATTCCCAAC
  TGTGACAAAAAGG
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001013398 unedited
  AAACTTTANTNTTTCCGCAGCGCCGGCGTGTGGNAAGCCCGTCCGTCTCCAGCACGCACC
  GGGTGTCTGATCCCAAGTTCCACCCCCTCCATTCAAAGATAATCATCATCAAGAAAGGGC
  ATGCTAAAGACAGCCAGCGCTACAAAGTTGACTACGAGTCTCAGAGCACAGATACCCAGA
  ACTTCTCCTCCGAGTCCAAGCGGGAGACAGAATATGGTCCCTGCCGTAGAGAAATGGAAG
  ACACACTGAATCACCTGAAGTTCCTCAATGTGCTGAGTCCCAGGGGTGTACACATTCCCA
  ACTGTGACAAGAAGGGATTTTATAAGAAAAAGCAGTGTCGCCCTTCCAAAGGCAGGAAGC
  GGGGCTTCTGCTGGTGTGTGGATAAGTATGGGCAGCCTCTCCCAGGCTACACCACCAAGG
  GGAAGGAGGACGTGCACTGCTACAGCATGCAGAGCAAGTAGACGCCTGCCGCAAGGTTAA
  TGTGGAGCTCAAATATGCCTTATTTTGCACAAAAGACTGCCAAGGACATGACCAGCAGCT
  GGCTACAGCCTCGATTTATATTTCTGTTTGTGGTGAACTGATTTTTTTTAAACCAAAGTT
  TAGAAAGAGGTTTTTGAAATGCCTATGGTTTCTTTGAATGGTAAACTTGAGCATCTTTTC
  ACTTTCCAGTAGTCAGCAAAGAGCAGTTTGAATTTTCTTGTCGCTTCCTATCAAAATATT
  CAGAGACTCGAGCACAGCACCCAGACTTCATGCGCCCGTGGAATGCTCACCACATGTTGG
  GTCGAAGCGGCCGACCACTGGACTTGTGACTTTAGCGGCTGTGNTGCCTATGTAGAAACA
  CGCTTCACCCCCACTCCCGTACGTGCGCACAGGGCTTATCGAAATAGGAAACCTTAAACC
  CCGTCATCCGGACTCN
  
• Restriction Sites: Please inquire
• ACCN: NM_001013398
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and insertion of 1 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013398.1, NP_001013416.1
• RefSeq Size: 2638 bp
• RefSeq ORF: 894 bp
• Locus ID: 3486
• UniProt ID: P17936
• Cytogenetics: 7p12.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: p53 signaling pathway
• Gene Summary: This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).