GDF6 (NM_001001557) Human Untagged Clone

CAT#: SC300216

GDF6 (untagged)-Human growth differentiation factor 6 (GDF6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GDF6
• Synonyms: BMP-13; BMP13; CDMP2; KFM; KFS; KFS1; KFSL; SGM1; SYNS4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001001557 edited
  ATGGATACTCCCAGGGTCCTGCTCTCGGCCGTCTTCCTCATCAGTTTTCTGTGGGATTTG
  CCCGGTTTCCAGCAGGCTTCCATCTCATCCTCCTCGTCGTCCGCCGAGCTGGGTTCCACC
  AAGGGCATGCGAAGCCGCAAGGAAGGCAAGATGCAGCGGGCGCCGCGCGACAGTGACGCG
  GGCCGGGAGGGCCAGGAACCACAGCCGCGGCCTCAGGACGAACCCCGGGCTCAGCAGCCC
  CGGGCGCAGGAGCCGCCAGGCAGGGGTCCGCGCGTGGTGCCCCACGAGTACATGCTGTCA
  ATCTACAGGACTTACTCCATCGCTGAGAAGCTGGGCATCAATGCCAGCTTTTTCCAGTCT
  TCCAAGTCGGCTAATACGATCACCAGCTTTGTAGACAGGGGACTAGACGATCTCTCGCAC
  ACTCCTCTCCGGAGACAGAAGTATTTGTTTGATGTGTCCATGCTCTCAGACAAAGAAGAG
  CTGGTGGGCGCGGAGCTGCGGCTCTTTCGCCAGGCGCCCTCAGCGCCCTGGGGGCCACCA
  GCCGGGCCGCTCCACGTGCAGCTCTTCCCTTGCCTTTCGCCCCTACTGCTGGACGCGCGG
  ACCCTGGACCCGCAGGGGGCGCCGCCGGCCGGCTGGGAAGTCTTCGACGTGTGGCAGGGC
  CTGCGCCACCAGCCCTGGAAGCAGCTGTGCTTGGAGCTGCGGGCCGCATGGGGCGAGCTG
  GACGCCGGGGAGGCCGAGGCGCGCGCGCGGGGACCCCAGCAACCGCCGCCCCCGGACCTG
  CGGAGTCTGGGCTTCGGCCGGAGGGTGCGGCCTCCCCAGGAGCGGGCCCTGCTGGTGGTA
  TTCACCAGATCCCAGCGCAAGAACCTGTTCGCAGAGATGCGCGAGCAGCTGGGCTCGGCC
  GAGGCTGCGGGCCCGGGCGCGGGCGCCGAGGGGTCGTGGCCGCCGCCGTCGGGCGCCCCG
  GATGCCAGGCCTTGGCTGCCCTCGCCCGGCCGCCGGCGGCGGCGCACGGCCTTCGCCAGT
  CGCCATGGCAAGCGGCACGGCAAGAAGTCCAGGCTACGCTGCAGCAAGAAGCCCCTGCAC
  GTGAACTTCAAGGAGCTGGGCTGGGACGACTGGATTATCGCGCCCCTGGAGTACGAGGCC
  TATCACTGCGAGGGTGTATGCGACTTCCCGCTGCGCTCGCACCTGGAGCCCACCAACCAC
  GCCATCATCCAGACGCTGATGAACTCCATGGACCCCGGCTCCACCCCGCCCAGCTGCTGC
  GTGCCCACCAAATTGACTCCCATCAGCATTCTATACATCGACGCGGGCAATAATGTGGTC
  TACAAGCAGTACGAGGACATGGTGGTGGAGTCGTGCGGCTGCAGGTAGCGGTGCCTTTCC
  CGCCGCCTTGGCCCGGAACCAAGGTGGGCCAAGGTCCGCCTTGCAGGGGAGGCCTGGCTG
  CAGAGAGGCGGAGGAGGAAGCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001001557 unedited
  GGGCGATTGTATACGACTCCTATAGGGCGGCGCGGGAATTCGCCCTTATGGATACTCCCA
  GGGTCCTGCTCTCGGCCGTCTTCCTCATCAGTTTTCTGTGGGATTTGCCCGGTTTCCAGC
  AGGCTTCCATCTCATCCTCCTCGTCGTCCGCCGAGCTGGGTTCCACCAAGGGCATGCGAA
  GCCGCAAGGAAGGCAAGATGCAGCGGGCGCCGCGCGACAGTGACGCGGGCCGGGAGGGCC
  AGGAACCACAGCCGCGGCCTCAGGACGAACCCCGGGCTCAGCAGCCCCGGGCGCAGGAGC
  CGCCAGGCAGGGGTCCGCGCGTGGTGCCCCACGAGTACATGCTGTCAATCTACAGGACTT
  ACTCCATCGCTGAGAAGCTGGGCATCAATGCCAGCTTTTTCCAGTCTTCCAAGTCGGCTA
  ATACGATCACCAGCTTTGTAGACAGGGGACTAGACGATCTCTCGCACACTCCTCTCCGGA
  GACAGAAGTATTTGTTTGATGTGTCCATGCTCTCAGACAAAGAAGAGCTGGTGGGCGCGG
  AGCTGCGGCTCTTTCGCCAGGCGCCCTCAGCGCCCTGGGGGCCACCAGCCGGGCCGCTCC
  ACGTGCAGCTCTTCCCTTGCCTTTCGCCCCTACTGCTGGACGCGCGGACCCTGGACCCGC
  AGGGGGCGCCGCCGGCCGGCTGGGAAGTCTTCGACGTGTGGCAGGGCCTGCGCCACCAGC
  CCTGGAAGCAGCTGTGCTTGGAGCTGCGGGCCGCATGGGGCGAGCTGGACGCCGGGGAGG
  CCGAGGCGCGCGCGCGGGGACCCCAGCAACCGCCGCCCCCGGACCTGCGGAGTCTGGGCT
  TCGGCCGGAGGGTGCGGCCTCCCCAGGAGCGGGCCCTGCTGGTGGTATTCACCAGATCCC
  AGCGCAAGACCTTGTTCGCAGAGAATGCCGCGAAGCAAGGCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_001001557
• Insert Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001557.1, NP_001001557.1
• RefSeq Size: 3716 bp
• RefSeq ORF: 1368 bp
• Locus ID: 392255
• UniProt ID: Q6KF10
• Cytogenetics: 8q22.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene are associated with Klippel-Feil syndrome, microphthalmia, and Leber congenital amaurosis. [provided by RefSeq, Sep 2016]