PLA2G1B (NM_000928) Human Untagged Clone

CAT#: SC300153

PLA2G1B (untagged)-Human phospholipase A2, group IB (pancreas) (PLA2G1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLA2G1B
• Synonyms: PLA2; PLA2A; PPLA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000928 edited
  ATGAAACTCCTTGTGCTAGCTGTGCTGCTCACAGTGGCCGCCGCCGACAGCGGCATCAGC
  CCTCGGGCCGTGTGGCAGTTCCGCAAAATGATCAAGTGCGTGATCCCGGGGAGTGACCCC
  TTCTTGGAATACAACAACTACGGCTGCTACTGTGGCTTGGGGGGCTCAGGCACCCCCGTG
  GATGAACTGGACAAGTGCTGCCAGACACATGACAACTGCTATGACCAGGCCAAGAAGCTG
  GACAGCTGTAAATTTCTGCTGGACAACCCGTACACCCACACCTATTCATACTCGTGCTCT
  GGCTCGGCAATCACCTGTAGCAGCAAAAACAAAGAGTGTGAGGCCTTCATTTGCAACTGC
  GACCGCAACGCTGCCATCTGCTTTTCAAAAGCTCCATATAACAAGGCACACAAGAACCTG
  GACACCAAGAAGTATTGTCAGAGTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000928 unedited
  NNNNGGTTTCGGGGTTCATCATTTGTNATACNACTCACTATAGGCGGCCGCGATTCATTC
  TGGTACCGAGCTCGGNATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCAC
  CTGGTCATCTCAGTTCTTTTCTCACCTTGACTGCAAGATGAAACTCCTTGTGCTAGCTGT
  GCTGCTCACAGTGGCCGCCGCCGACAGCGGCATCAGCCCTCGGGCCGTGTGGCAGTTCCG
  CAAAATGATCAAGTGCGTGATCCCGGNGAGTGACCCCTTCTTGGAATACAACAACTACGG
  CTGCTACTGTGGCTTGGGGGGCTCAGGCACCCNCGTGGATGAACTGGACAAGTGCTGCCA
  GACACATNGACACTGCTATGACCAGGCCAAGAAGCTGGACAGCTGTAAATTTCTGCTGGA
  CAACCCGTACACCCACACCTATTCATACTCGTGCTCTGGCTCGGCAATCACCTGTAGCAG
  CAAAAACAAAGAGTGTGAGGCCTTCATTTGCAACTGCGACCGCAACGCTGCCATCTGCTT
  TTCAAAAGCTCCATATAACAAGGCACACATGAACCCTGGACACCAGAAGTATTGTCAGAG
  TTGATATCACCTCTCAAAAAGCATCACTCTATCTGCCTCATCTCACACTGTACTCTCCAA
  TAAGCACCCTGTTTGAAAGACCTCATTGATGAAGGCCGAATTCTGCAGAATTCCTCACAC
  TGGCGGGCGGTCGAGCATGCATTTAGATTGCGGCCCCGGTCATAGCTGTTTCCTGAACAG
  ATCCCGGGTGGCATCCCTGTGACCCTTCCCCCATGGCTTTTCTGGCCCTGTGGGTTGGCC
  ACTCCAGGGCCAACCACCCCTGTCCCAATAAAATTAATTTGCTCATTT
  
• Restriction Sites: Please inquire
• ACCN: NM_000928
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000928.2, NP_000919.1
• RefSeq Size: 585 bp
• RefSeq ORF: 447 bp
• Locus ID: 5319
• UniProt ID: P04054
• Cytogenetics: 12q24.31
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]