Thyroid Peroxidase (TPO) (NM_000547) Human Untagged Clone

CAT#: SC300094

TPO (untagged)-Human thyroid peroxidase (TPO), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thyroid Peroxidase
• Synonyms: MSA; TDH2A; TPX
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000547 edited
  AAGGCAATTAAGGCGCCCATTTCAGAAGAGTTACAGCCGTGAAAATTACTCAGCAGTGCA
  GTTGGCTGAGAAGAGGAAAAAAGAATGAGAGCGCTGGCTGTGCTGTCTGTCACGCTGGTT
  ATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCTCGAGAGGGAAAGAACTCCTTTGGGGA
  AAGCCTGAGGAGTCTCGTGTCTCTAGCGTCTTGGAGGAAAGCAAGCGCCTGGTGGACACC
  GCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTGCAGCTCAG
  CTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAG
  ATAATGGAAACATCAATACAAGCGATGAAAAGAAAAGTCAACCTGAAAACTCAACAATCA
  CAGCATCCAACGGATGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGA
  TGTCTCCCTTACATGCTGCCCCCAAAATGCCCAAACACTTGCCTGGCGAACAAATACAGG
  CCCATCACAGGAGCTTGCAACAACAGAGACCACCCCAGATGGGGCGCCTCCAACACGGCC
  CTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCAGCCCCGAGGCTGGAAC
  CCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCCGGGAGGTGACAAGACATGTC
  ATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCA
  TGGGGACAATACATCGACCACGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCC
  TTCGGGGGAGGGGCTGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATA
  CAACTCCCGGAGGAGGCCCGGCCGGCCGCGGGCACCGCCTGTCTGCCCTTCTACCGCTCT
  TCGGCCGCCTGCGGCACCGGGGACCAAGGCGCGCTCTTTGGGAACCTGTCCACGGCCAAC
  CCGCGGCAGCAGATGAACGGGTTGACCTCGTTCCTGGACGCGTCCACCGTGTATGGCAGC
  TCCCCGGCCCTAGAGAGGCAGCTGCGGAACTGGACCAGTGCCGAAGGGCTGCTCCGCGTC
  CACGCGCGCCTCCGGGACTCCGGCCGCGCCTACCTGCCCTTCGTGCCGCCACGCGCGCCT
  GCGGCCTGTGCGCCCGAGCCCGGCATCCCCGGAGAGACCCGCGGGCCCTGCTTCCTGGCC
  GGAGACGGCCGCGCCAGCGAGGTCCCCTCCCTGACGGCACTGCACACGCTGTGGCTGCGC
  GAGCACAACCGCCTGGCCGCGGCGCTCAAGGCCCTCAATGCGCACTGGAGCGCGGACGCC
  GTGTACCAGGAGGCGCGCAAGGTCGTGGGCGCTCTGCACCAGATCATCACCCTGAGGGAT
  TACATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGTGGGTCCCTATGAAGGC
  TATGACTCCACCGCCAACCCCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTCCGCTTC
  GGCCATGCCACGATCCACCCGCTGGTGAGGAGGCTGGACGCCAGCTTCCAGGAGCACCCC
  GACCTGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTACTCCGTGGA
  GGTGGTTTGGACCCACTAATACGAGGCCTTCTTGCAAGACCAGCCAAACTGCAGGTGCAG
  GATCAGCTGATGAACGAGGAGCTGACGGAAAGGCTCTTTGTGCTGTCCAATTCCAGCACC
  TTGGATCTGGCGTCCATCAACCTGCAGAGGGGCCGGGACCACGGGCTGCCAGGTTACAAT
  GAGTGGAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCCCCGCTGACCTGAGCACAGCC
  ATCGCCAGCAGGAGCGTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATC
  GATGTCTGGCTGGGAGGCTTAGCTGAAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTG
  TTTGCCTGTCTCATTGGGAAGCAGATGAAGGCTCTGCGGGACGGTGACTGGTTTTGGTGG
  GAGAACAGCCACGTCTTCACGGATGCACAGAGGCGTGAGCTGGAGAAGCACTCCCTGTCT
  CGGGTCATCTGTGACAACACTGGCCTCACCAGGGTGCCCATGGATGCCTTCCAAGTCGGC
  AAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGAACCTGGAGGCCTGG
  AGGGAAACCTTTCCTCAAGACGACAAGTGTGGCTTCCCAGAGAGCGTGGAGAATGGGGAC
  TTTGTGCACTGTGAGGAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCACGGGTAT
  GAGCTCCAAGGCCGGGAGCAGCTCACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCC
  CTCTGCAAAGATGTGAACGAGTGTGCAGACGGTGCCCACCCCCCCTGCCACGCCTCTGCG
  AGGTGCAGAAACACCAAAGGCGGCTTCCAGTGTCTCTGCGCGGACCCCTACGAGTTAGGA
  GACGATGGGAGAACCTGCGTAGACTCCGGGAGGCTCCCTCGGGTGACTTGGATCTCCATG
  TCGCTGGCTGCTCTGCTGATCGGAGGCTTCGCAGGTCTCACCTCGACGGTGATTTGCAGG
  TGGACACGCACTGGCACTAAATCCACACTGCCCATCTCGGAGACAGGCGGAGGAACTCCC
  GAGCTGAGATGCGGAAAGCACCAGGCCGTAGGGACCTCACCGCAGCGGGCCGCAGCTCAG
  GACTCGGAGCAGGAGAGTGCTGGGATGGAAGGCCGGGATACTCACAGGCTGCCGAGAGCC
  CTCTGAGGGCAAAGTGGCAGGACACTGCAGAACAGCTTCATGTTCCCAAAATCACCGTAC
  GACTCTTTTCCAAACACAGGCAAATCGGAAATCAGCAGGACGACTGTTTTCCCAACACGG
  GTAAATCTAGTACCATGTCGTAGTTACTCTCAGGCATGGATGAATAAATGTTATAGCTGT
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000547
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_000547.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000547.3, NP_000538.3
• RefSeq Size: 3135 bp
• RefSeq ORF: 2802 bp
• Locus ID: 7173
• UniProt ID: P07202
• Cytogenetics: 2p25.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Metabolic pathways, Tyrosine metabolism
• Gene Summary: This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a, also known as TPO1).