PNMT (NM_002686) Human Untagged Clone

CAT#: SC128283

PNMT (untagged)-Human phenylethanolamine N-methyltransferase (PNMT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PNMT
• Synonyms: PENT; PNMTase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002686 edited
  GGACGATTGCCGCTGCAGCCGCCGCCCCACTCACCTCCGGTGTGTCTGCAGCCCGGACAC
  TAAGGGAGATGGATGAATGGGTGGGGAGGATGCGGCGCACATGGCCCCGGGCGGCTCGGC
  GGTCAGCTGCCGCCCCCACAGCGGACCGGTCGGGGCGGGGGTCGGGCGGTAGAAAAAAGG
  GCCGCGAGGCGAGCGGGGCACTGGGCGGACCGCGGCGGCAGCATGAGCGGCGCAGACCGT
  AGCCCCAATGCGGGCGCAGCCCCTGACTCGGCCCCGGGCCAGGCGGCGGTGGCTTCGGCC
  TACCAGCGCTTCGAGCCGCGCGCCTACCTCCGCAACAACTACGCGCCCCCTCGCGGGGAC
  CTGTGCAACCCGAACGGCGTCGGGCCGTGGAAGCTGCGCTGCTTGGCGCAGACCTTCGCC
  ACCGGTGAAGTGTCCGGACGCACCCTCATCGACATTGGTTCAGGCCCCACCGTGTACCAG
  CTGCTCAGTGCCTGCAGCCACTTTGAGGACATCACCATGACAGATTTCCTGGAGGTCAAC
  CGCCAGGAGCTGGGGCGCTGGCTGCAGGAGGAGCCGGGGGCCTTCAACTGGAGCATGTAC
  AGCCAACATGCCTGCCTCATTGAGGGCAAGGGGGAATGCTGGCAGGATAAGGAGCGCCAG
  CTGCGAGCCAGGGTGAAACGGGTCCTGCCCATCGACGTGCACCAGCCCCAGCCCCTGGGT
  GCTGGGAGCCCAGCTCCCCTGCCTGCTGACGCCCTGGTCTCTGCCTTCTGCTTGGAGGCT
  GTGAGCCCAGATCTTGCCAGCTTTCAGCGGGCCCTGGACCACATCACCACGCTGCTGAGG
  CCTGGGGGGCACCTCCTCCTCATCGGGGCCCTGGAGGAGTCGTGGTACCTGGCTGGGGAG
  GCCAGGCTGACGGTGGTGCCAGTGTCTGAGGAGGAGGTGAGGGAGGCCCTGGTGCGTAGT
  GGCTACAAGGTCCGGGACCTCCGCACCTATATCATGCCTGCCCACCTTCAGACAGGCGTA
  GATGATGTCAAGGGCGTCTTCTTCGCCTGGGCTCAGAAGGTTGGGCTGTGAGGGCTGTAC
  CTGGTGCCCTGTGGCCCCCACCCACCTGGATTCCCTGTTCTTTGAAGTGGCACCTAATAA
  AGAAATAATACCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002686 unedited
  GGTACACATTGTACACGACCTCCTACTAGGGCGGCCGCGAATTCCCGGGATGGACGATTG
  CCGCTGCAGCCGCCGCCCCACTCACCTCCGGTGTGTCTGCAGCCCGGACACTAAGGGAGA
  TGGATGAATGGGTGGGGAGGATGCGGCGCACATGGCCCCGGGCGGCTCGGCGGTCAGCTG
  CCGCCCCCACAGCGGACCGGTCGGGGCGGGGGTCGGGCGGTAGAAAAAAGGGCCGCGAGG
  CGAGCGGGGCACTGGGCGGACCGCGGCGGCAGCATGAGCGGCGCAGACCGTAGCCCCAAT
  GCGGGCGCAGCCCCTGACTCGGCCCCGGGCCAGGCGGCGGTGGCTTCGGCCTACCAGCGC
  TTCGAGCCGCGCGCCTACCTCCGCAACAACTACGCGCCCCCTCGCGGGGACCTGTGCAAC
  CCGAACGGCGTCGGGCCGTGGAAGCTGCGCTGCTTGGCGCAGACCTTCGCCACCGGTGAA
  GTGTCCGGACGCACCCTCATCGACATTGGTTCAGGCCCCACCGTGTACCAGCTGCTCAGT
  GCCTGCAGCCACTTTGAGGACATCACCATGACAGATTTCCTGGAGGTCAACCGCCAGGAG
  CTGGGGCGCTGGCTGCAGGAGGAGCCGGGGGCCTTCAACTGGAGCATGTACAGCCAACAT
  GCCTGCCTCATTGAGGGCAAGGGGGAATGCTGGCAGGATAAGGAGCGCCAGCTGCGAGCC
  AGGGTGAAACGGGTCCTGCCCATCGACGTGCACCAGCCCCAGCCCCTGGGTGCTGGGAGC
  CCAGCTCCCCTGCCTGCTGACGCCCTGGTCTCTGCCTTCTGCTTGGAGGCTGTGAGCCCA
  GATCTTGCCAGCTTTCAGCGGGCCCTGACCACATCACCACGCTGCTGAGCCCTGGGGGGG
  CACTCTCTCATCGGGGCCTGAGAGTCGTGTTACTGCTGGGAGCAGCTGACGTGTGCCCAG
  TTGTTCCTGAGAGAGGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_002686
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002686.3, NP_002677.1
• RefSeq Size: 1177 bp
• RefSeq ORF: 849 bp
• Locus ID: 5409
• UniProt ID: P11086
• Cytogenetics: 17q12
• Domains: NNMT_PNMT_TEMT
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism
• Gene Summary: The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) encodes a functional protein.