BMP8B (NM_001720) Human Untagged Clone

CAT#: SC128142

BMP8B (untagged)-Human bone morphogenetic protein 8b (BMP8B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BMP8B
• Synonyms: BMP8; OP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001720, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCGCGCTCCCCGGCCCGCTCTGGCTCCTGGGCCTGGCGCTATGCGCGCTGGGCGGGGGCGGCCCCG
  GCCTGCGACCCCCGCCCGGCTGTCCCCAGCGACGTCTGGGCGCGCGCGAGCGCCGGGACGTGCAGCGCGA
  GATCCTGGCGGTGCTCGGGCTGCCTGGGCGGCCCCGGCCCCGCGCGCCACCCGCCGCCTCCCGGCTGCCC
  GCGTCCGCGCCGCTCTTCATGCTGGACCTGTACCACGCCATGGCCGGCGACGACGACGAGGACGGCGCGC
  CCGCGGAGCGGCGCCTGGGCCGCGCCGACCTGGTCATGAGCTTCGTTAACATGGTGGAGCGAGACCGTGC
  CCTGGGCCACCAGGAGCCCCATTGGAAGGAGTTCCGCTTTGACCTGACCCAGATCCCGGCTGGGGAGGCG
  GTCACAGCTGCGGAGTTCCGGATTTACAAGGTGCCCAGCATCCACCTGCTCAACAGGACCCTCCACGTCA
  GCATGTTCCAGGTGGTCCAGGAGCAGTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCT
  CCGAGCTGGAGACGAGGGCTGGCTGGTGCTGGATGTCACAGCAGCCAGTGACTGCTGGTTGCTGAAGCGT
  CACAAGGACCTGGGACTCCGCCTCTATGTGGAGACTGAGGACGGGCACAGCGTGGATCCTGGCCTGGCCG
  GCCTGCTGGGTCAACGGGCCCCACGCTCCCAACAGCCTTTCGTGGTCACTTTCTTCAGGGCCAGTCCGAG
  TCCCATCCGCACCCCTCGGGCAGTGAGGCCACTGAGGAGGAGGCAGCCGAAGAAAAGCAACGAGCTGCCG
  CAGGCCAACCGACTCCCAGGGATCTTTGATGACGTCCACGGCTCCCACGGCCGGCAGGTCTGCCGTCGGC
  ACGAGCTCTACGTCAGCTTCCAGGACCTCGGCTGGCTGGACTGGGTCATCGCTCCCCAAGGCTACTCGGC
  CTATTACTGTGAGGGGGAGTGCTCCTTCCCACTGGACTCCTGCATGAATGCCACCAACCACGCCATCCTG
  CAGTCCCTGGTGCACCTGATGATGCCAGACGCAGTCCCCAAGGCGTGCTGTGCACCCACCAAGCTGAGCG
  CCACCTCTGTGCTCTACTATGACAGCAGCAACAATGTCATCCTGCGCAAGCACCGCAACATGGTGGTCAA
  GGCCTGCGGCTGCCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001720 unedited
  GGCTCTCTTGGNNGGAGTATANAATTTGTNATACNACTCACTATAGGCGGCCGCGATTCC
  GAGCCCAGCCTCCTTGCCGTCGGGGCGTCCCCAGGCCCTGGGTCGGCCGCGGAGCCGATG
  CGCGCCCGCTGAGCGCCCCAGCTGAGCGCCCCCGGCCTGCCATGACCGCGCTCCCCGGCC
  CGCTCTGGCTCCTGGGCCTGGCGCTATGCGCGCTGGGCGGGGGCGGCCCCGGCCTGCGAC
  CCCCGCCCGGCTGTCCCCAGCGACGTCTGGGCGCGCGCGAGCGCCGGGACGTGCAGCGCG
  AGATCCTGGCGGTGCTNCGGCTGCCTGGGCGGGCCCGGNNCCGCGCGCCACCCGNCGNCT
  NCCGGCTGCCCGCGTNCGCGCCGCTCTTCATGCTGGACCTGTACCACGCCATGGCCCGCG
  ACNACCACNAGGACCGCGCGGCCGCGGAAGCGGGCCTGGGCCCGGCCCCACTGGTCATGA
  ACTTCGTCACTGGTGGGACGAGACCGTGCCTGGGGCCCCAGAGCCCATTGGGAAGAATTT
  CGCTTTGACCTGGCCAAATCCCGCTTGGGAGGCGGTACACGTTCGGATTCCGGATTACAA
  GGGGCCAAGATTCACCGGTTTAAAGGGCCCTCCAGTTAGATGTTCCAAGGGGGCCCGGAG
  CCATCCCAAGGGGGGTTGGCTGGTCCTTTTGGATTTTAAAAACCCCCAACCTGGAAACAA
  ACCTCCCTTGGCCCCGTTTTCAAAACACCCCACTGTCCGTGGTTTTTTAAAAAGCTCAAA
  GACCCGGGGGACCCCCCCTTTTTTGGAACCTAGGTGTGGGCCCCCGTGGTTTTCCGTTCG
  GCCCCCCCCTTTGTTTA
  
• Restriction Sites: Please inquire
• ACCN: NM_001720
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001720.3, NP_001711.2
• RefSeq Size: 3773 bp
• RefSeq ORF: 1209 bp
• Locus ID: 656
• UniProt ID: P34820
• Cytogenetics: 1p34.2
• Domains: TGFb_propeptide, TGF-beta
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway
• Protein Pathways: Hedgehog signaling pathway, TGF-beta signaling pathway
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The encoded protein stimulates thermogenesis in brown adipose tissue. Expression of this gene may be downregulated in pancreatic cancer. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1. [provided by RefSeq, Jul 2016]