IREB2 (NM_004136) Human Untagged Clone

CAT#: SC128101

IREB2 (untagged)-Human iron-responsive element binding protein 2 (IREB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IREB2
• Synonyms: ACO3; IRE-BP 2; IRE-BP2; IRP2; IRP2AD; NDCAMA
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128101 sequence for NM_004136 edited (data generated by NextGen Sequencing)
  ATGGACGCCCCAAAAGCAGGATACGCCTTTGAGTACCTTATTGAAACATTAAATGACAGT
  TCACATAAGAAGTTCTTCGATGTATCTAAACTTGGCACCAAGTATGATGTTCTGCCTTAC
  TCAATACGGGTCTTGTTGGAAGCTGCTGTACGAAATTGTGATGGCTTTTTAATGAAGAAG
  GAAGATGTTATGAACATTTTAGACTGGAAAACCAAACAAAGCAATGTTGAAGTGCCCTTT
  TTCCCTGCCCGTGTTCTTCTTCAAGATTTTACTGGAATACCAGCAATGGTGGATTTTGCT
  GCTATGAGGGAGGCAGTGAAAACTCTTGGAGGTGATCCTGAGAAAGTCCATCCTGCTTGT
  CCGACAGATCTTACAGTTGACCATTCTTTACAAATTGACTTCAGTAAATGTGCAATACAG
  AATGCACCAAATCCTGGAGGTGGTGACCTGCAGAAAGCAGGAAAGCTCTCTCCACTTAAA
  GTGCAGCCTAAGAAGCTTCCCTGCAGAGGCCAGACTACCTGCCGAGGATCTTGTGATTCT
  GGAGAACTAGGCCGAAACTCAGGAACATTTTCTTCGCAGATTGAGAATACACCCATCCTG
  TGTCCTTTTCATTTGCAACCAGTGCCTGAACCTGAAACAGTGTTAAAAAATCAAGAAGTA
  GAATTCGGCAGAAATCGAGAGAGGCTTCAGTTTTTTAAGTGGAGTTCAAGAGTTTTTAAG
  AATGTGGCAGTGATCCCTCCTGGAACTGGAATGGCTCATCAAATAAACTTAGAATATTTG
  TCAAGAGTGGTTTTTGAAGAAAAAGACCTCCTCTTCCCAGACAGTGTAGTCGGCACAGAT
  TCACACATAACGATGGTGAATGGTTTAGGGATTCTGGGGTGGGGGGTTGGAGGCATTGAA
  ACAGAAGCAGTTATGCTTGGTCTGCCAGTTTCTCTTACTTTACCAGAGGTGGTTGGATGT
  GAGTTAACTGGGTCATCAAACCCTTTTGTTACATCCATAGATGTTGTTCTTGGTATTACA
  AAGCACCTCAGGCAAGTAGGAGTGGCTGGAAAGTTTGTTGAGTTTTTTGGAAGTGGAGTT
  TCACAATTATCTATAGTTGATCGAACTACAATAGCAAACATGTGTCCGGAATATGGTGCT
  ATCCTCAGCTTTTTCCCTGTTGACAATGTGACATTAAAACATTTAGAACATACAGGTTTT
  AGCAAAGCCAAACTCGAATCAATGGAAACATACCTTAAAGCTGTGAAATTGTTTCGAAAT
  GACCAGAATTCTTCAGGAGAACCTGAATACTCCCAGGTGATCCAGATTAATCTGAATTCA
  ATAGTTCCATCTGTTAGTGGTCCAAAAAGACCTCAGGATAGAGTTGCTGTGACAGATATG
  AAAAGCGATTTCCAGGCTTGCTTAAATGAAAAGGTTGGATTTAAAGGCTTCCAAATTGCA
  GCTGAAAAACAAAAGGATATTGTCTCCATTCATTATGAAGGAAGTGAATATAAGCTGTCT
  CATGGATCAGTGGTCATTGCTGCAGTTATCAGTTGTACCAATAATTGCAATCCATCTGTC
  ATGCTTGCTGCAGGTCTTTTGGCTAAAAAGGCTGTTGAAGCTGGTCTGCGTGTTAAACCT
  TATATAAGAACAAGTTTATCTCCAGGCAGTGGGATGGTTACACATTACCTCAGTTCAAGT
  GGAGTATTACCATATCTAAGTAAGCTTGGATTTGAAATCGTTGGCTATGGATGTTCAACT
  TGTGTGGGAAATACAGCACCCTTATCAGACGCAGTTTTAAATGCAGTAAAACAGGGTGAT
  TTGGTTACCTGTGGAATTTTATCTGGAAACAAAAATTTTGAAGGTCGTCTTTGTGATTGT
  GTTCGTGCCAATTATCTTGCCTCTCCACCCTTAGTGGTAGCTTATGCCATAGCAGGCACA
  GTGAATATAGATTTCCAGACAGAACCTTTAGGTACTGACCCCACCGGCAAGAACATTTAC
  CTGCATGATATTTGGCCTAGTCGAGAAGAAGTTCATCGAGTAGAGGAAGAACATGTTATA
  CTATCCATGTTTAAAGCATTAAAAGATAAAATAGAAATGGGGAATAAACGGTGGAATTCC
  TTAGAAGCACCGGATTCAGTTTTGTTTCCATGGGACTTAAAGTCTACTTATATCAGATGC
  CCTTCATTTTTTGATAAACTTACCAAAGAGCCAATTGCACTCCAGGCTATTGAAAATGCC
  CATGTCTTATTATATTTGGGAGACTCTGTCACAACAGATCATATATCACCTGCAGGAAGT
  ATCGCTAGGAATAGTGCTGCCGCTAAGTATTTGACAAACAGAGGCCTTACCCCTCGTGAA
  TTCAACTCTTACGGAGCTCGAAGAGGTAATGATGCTGTAATGACAAGAGGCACTTTTGCA
  AATATCAAGCTTTTTAATAAGTTTATTGGAAAACCAGCTCCTAAAACAATTCATTTTCCA
  TCAGGACAGACGCTAGATGTATTTGAGGCTGCAGAGCTGTACCAGAAAGAAGGTATCCCA
  CTGATTATTTTAGCAGGAAAGAAATATGGTTCAGGAAACTCCAGAGACTGGGCTGCCAAA
  GGACCGTATTTACTGGGTGTGAAAGCTGTTTTGGCTGAAAGTTATGAAAAAATACACAAA
  GATCATTTGATTGGAATTGGCATAGCTCCACTTCAGTTCCTTCCAGGAGAAAATGCAGAT
  TCCTTGGGCCTCTCCGGTAGAGAAACATTTTCTTTAACATTTCCTGAAGAACTGTCTCCT
  GGAATTACATTGAATATACAGACAAGCACTGGAAAAGTATTCAGCGTGATTGCTTCGTTT
  GAAGATGATGTGGAAATAACATTATACAAACATGGAGGATTATTAAACTTTGTGGCACGA
  AAATTCTCATAG
  
  Clone variation with respect to NM_004136.2
  2616 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004136 unedited
  CAATTCCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGCCCTTCTTCCCCCGCTGGCCCCCTCCCCGGAGGGATAATATGGT
  CTCCGGCGATGGACGCCCCAAAAGCAGGATACGCCTTTGAGTACCTTATTGAAACATTAA
  ATGACAGTTCACATAAGAAGTTCTTCGATGTATCTAAACTTGGCACCAAGTATGATGTTC
  TGCCTTACTCAATACGGGTCTTGTTGGAAGCTGCTGTACGAAATTGTGATGGCTTTTTAA
  TGAAGAAGGAAGATGTTATGAACATTTTAGACTGGAAAACCAAACAAAGCAATGTTGAAG
  TGCCCTTTTTCCCTGCCCGTGTTCTTCTTCAAGAGTTTACTGGAATACCAGCAATGGTGG
  ATTTTGCTGCTATGAGGGAGGCAGTGAAAACTCTTGGAGGTGATCCTGAGAAAGTCCATC
  CTGCTTGTCCGACAGATCTTACAGTTGACCATTCTTTACAAATTGACTTCAGTAAATGTG
  CAATACAGAATGCACCAAATCCTGGAGGTGGTGACCTGCAGAAAGCAGGAAAGCTCTCTC
  CACTTAAAGTGCAGCCTAAGAAGCTTCCCTGCAGAGGCCAGACTACCTGCCGAGGATCTT
  GTGATTCTGGAGACTAGGCCGAAACTCAGGACATTTTCTTCGCAGATTGAGAGACACCCA
  TNCTGTGTCCTTTTCATTTGCAACCAGTGCCCTGACCTGAAACAGTGTTAAANCATCAAG
  AAGTAGTATTCTGCTGACATCGAGAGAGGCTTCAGTTNTTTCAAGTGGAGTTCAAGACGT
  TTTCGAGTGTGGCAGTGATCCTTCTGNAAACTGCATGGCTCATCCAATAACTTAGATAAT
  TGTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_004136 unedited
  TAGGATCGAGTTTTTTTTTTTTTTTTTTGGTAACACAAAATTACCCAAGGTATAGTAAAC
  AAAGAAGACACAGACAATAAAAACTAACAGATGCCACATTTTGCAAACATAGCATTTGGC
  TAGAGGCAGAATTATAAGGTATACATATTAAAACATACATCCNAGACTCTCCTCCTCAAT
  TTGAACCAATGTTATCAAGTTTTACTTCATGNNTATAGCCAAAAAAATTAGAGAACTTGA
  TTTGTCCTGAAATCAGAATTGCTTGATTATTACTGACCAGTNAACTGGAATGGGAAGTTT
  AATCAAAGTTGCCAGGTTTACACCACCCCCGAAAACCTGCGGGGAGACCGTGGGCCGGCC
  CAAAAAATTATAAAGATGCTTTTAAAAACATAGCCGCCGGTGGGGGCCCACCCGGATCCC
  ACCTTTGGGGGCCCAGGGGGGGGTTCCAGGGCGAGAAATTTTATTTGGCGCCCCGGGGAA
  ACCCCCCCTCCAAAAAACAAAAAAAAATGGGCGGGGGGGGCACCCCTTTTCCCCCCCCCC
  CGGAGGGGGGGGGGGGGAACCCCCCCCCCCCGGGGCGGGGTTTTTTTAAAAAAAAATACC
  CTTTTTTTTCCCCCCCCCCCGGGGGAACAACCCTCTAAAAAAAACCACCCAAATTTTTTT
  TTTTCCCCCCCAACAGGGGGAAAATTTTTTTTAAAAAAACAAAGGGTTTTTTCTTCTCCG
  GGTGTTTCCCCCCAACGGTTTTGGAGGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004136
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004136.1, NP_004127.1
• RefSeq Size: 3928 bp
• RefSeq ORF: 2892 bp
• Locus ID: 3658
• UniProt ID: P48200
• Cytogenetics: 15q25.1
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]