EXOC8 (NM_175876) Human Untagged Clone

SKU SC128095

EXOC8 (untagged)-Human exocyst complex component 8 (EXOC8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EXOC8
• Synonyms: EXO84; Exo84p; NEDMISB; SEC84
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_175876, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGATGGCGATGTCGGACAGTGGGGCGAGCCGCCTGCGTCGGCAGCTGGAGTCAGGGGGTTTTGAGG; CGCGGCTGTACGTGAAGCAGCTCTCGCAGCAGTCGGATGGGGACCGGGACCTCCAGGAGCACCGGCAGCG; CATCCAGGCGCTGGCGGAGGAGACGGCGCAGAACCTGAAGCGCAACGTCTACCAGAACTACCGGCAGTTC; ATAGAGACGGCCCGCGAGATCTCCTACCTGGAGAGCGAGATGTACCAGCTCAGCCATTTGCTGACCGAGC; AGAAAAGCAGCCTGGAGAGCATCCCGCTTACGTTGCTGCCTGCCGCTGCTGCCGCCGGAGCCGCCGCCGC; CTCTGGAGGGGAGGAGGGAGTCGGTGGGGCGGGGGGCCGAGACCACCTCCGAGGCCAGGCCGGCTTTTTC; TCCACCCCCGGGGGTGCCTCCCGCGACGGCTCCGGTCCAGGCGAGGAAGGAAAGCAGCGCACTCTCACCA; CCCTGCTTGAGAAGGTGGAAGGCTGCAGGCATCTGCTGGAGACGCCGGGACAGTACTTGGTGTACAATGG; GGACCTAGTGGAATACGATGCGGACCACATGGCCCAACTGCAGCGGGTGCACGGCTTTCTCATGAACGAT; TGCTTGTTGGTGGCTACCTGGCTGCCTCAGCGGCGTGGGATGTATCGCTACAACGCTCTCTATTCCCTAG; ATGGTTTGGCCGTAGTCAATGTCAAGGACAACCCGCCCATGAAGGACATGTTCAAGCTGCTTATGTTCCC; CGAGAGCCGTATTTTCCAGGCCGAAAATGCTAAAATCAAACGAGAGTGGCTGGAAGTGCTGGAGGACACC; AAGAGGGCCCTCAGTGAGAAAAGGCGAAGGGAGCAGGAGGAGGCAGCGGCCCCTCGAGGGCCACCCCAAG; TGACTTCCAAGGCCACTAACCCATTTGAGGATGACGAAGAAGAAGAACCAGCTGTTCCTGAGGTAGAGGA; AGAGAAGGTGGACCTCTCCATGGAATGGATCCAGGAGTTACCTGAAGACCTGGATGTCTGCATTGCGCAG; AGAGACTTTGAAGGGGCGGTTGACCTGCTGGATAAATTGAACCATTACCTGGAAGATAAACCTAGCCCAC; CTCCTGTAAAAGAACTAAGGGCCAAAGTGGAGGAGCGAGTTCGACAGCTCACTGAGGTGCTAGTTTTCGA; ACTCTCCCCAGATCGTTCCCTGAGAGGTGGTCCGAAGGCTACTCGCAGAGCAGTTTCGCAACTGATCCGG; CTGGGCCAGTGCACGAAGGCCTGTGAGCTATTTTTGAGAAACAGGGCAGCCGCTGTTCATACTGCAATTC; GTCAGCTTCGCATCGAAGGTGCCACTTTACTCTATATTCATAAGCTGTGCCATGTCTTCTTTACCAGCCT; TCTCGAGACTGCAAGAGAATTTGAGATCGATTTTGCAGGCACTGACAGCGGCTGCTACTCTGCCTTTGTG; GTCTGGGCAAGATCAGCCATGGGCATGTTCGTGGATGCTTTTAGCAAGCAGGTGTTTGATAGTAAGGAGA; GCCTCTCTACAGCAGCTGAGTGTGTAAAAGTGGCTAAGGAGCATTGCCAGCAACTGGGTGATATCGGACT; GGATCTCACCTTCATCATCCATGCCCTTCTGGTGAAAGACATCCAAGGGGCCTTGCACAGTTACAAAGAA; ATCATCATTGAAGCCACTAAACATCGCAACTCTGAAGAGATGTGGAGGAGGATGAACTTGATGACGCCAG; AAGCCCTGGGTAAGCTCAAAGAAGAGATGAAAAGTTGTGGGGTAAGTAACTTTGAGCAGTACACAGGGGA; TGACTGCTGGGTGAACCTAAGTTACACAGTGGTTGCTTTCACCAAACAGACCATGGGCTTCTTGGAAGAG; GCCCTGAAGCTGTATTTCCCAGAGCTGCACATGGTACTTTTGGAGAGCCTGGTGGAAATCATTTTGGTTG; CTGTTCAGCATGTGGATTATAGTCTTCGATGTGAGCAGGATCCAGAGAAGAAAGCTTTTATCAGACAGAA; TGCATCCTTTTTATATGAAACAGTCCTCCCTGTGGTGGAGAAAAGGTTTGAAGAAGGTGTGGGGAAACCT; GCCAAGCAACTCCAAGATCTGAGGAATGCATCTAGACTTATTCGTGTGAATCCTGAAAGTACAACATCAG; TGGTCTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175876 unedited; AATTCGGCACGAGGGACAGCCGCGGCGGCTGCGGCGCCGATAGTGACAGTGAGTGCGTGA; GACCCGGAGAGAAATGGCGATGGCGATGTCGGACAGTGGGGCGAGCCGCCTGCGTCGGCA; GCTGGAGTCAGGGGGTTTTGAGGCGCGGCTGTACGTGAAGCAGCTCTCGCAGCAGTCGGA; TGGGGACCGGGCTCTCCAGGAGCACCGGCAGCGCATCCAGGCGCTGGCGGAGGAGACGGC; GCAGAACCTGAAGCGCAACGTCTACCAGAACTACCGGCAGTTCATAGAGACGGCCCGCGA; GATCTCCTACCTGGAGAGCGAGATGTACCAGCTCAGCCATTTGCTGACCGAGCAGAAAAG; CAGCCTGGAGAGCATCCCGCTTACGTTGCTGCCTGCCGCTGCTGCCGCCGGAGCCGCCGC; CGCCTCTGGAGGGGAGGAGGGAGTCGGTGGGGCGGNGGGCCGAGACCACCTCCGAGGCCA; GGCCGGCTTTTTCTCCACCCCCGGNGGTGCCTCCCGCGACGGCTCCGGTCCAGGCGAGGA; AGGANAGCAGCGCACTCTCACCACCCTGCTTGAGAAGGTGGAAGGCTGCAGGCATCTGCT; GGAGACGCCGGGACAGTACTTGGTGTACAATGGGGACCTAGTGGAATACGATGCGGACCA; CATGGCCCAACTGCAGCGGGTGCACGGCTTTCTCATGAACGATTGCTTGTTGGTGGCTAC; CTGGCTGCCTCAGCGGCGTGGGATGTAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_175876 unedited; GGAATTCTAGGACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT; TTAAAACAATTTTTAAGCTTTATTTTTATGAAACATTTCAGATTCCCTCATATCACAGCA; CATCAATAAGCAGTATGTACATAGACTGACTTTTATAGTACGTGTCATGTCCCAAATTCC; CAATCCTAGGTAAGATATCAAGTTACAAAATACAAGTGCCGATAATTAAACTATAGGTAG; TATATTAAACAAAAATGAGTTTTTAGCAATTATGTGAAATAAGGCTTTAACCAAAGCAAG; ACTTAATGCCACAAGGTGTCTTTTTTCCCCAAGAGTAATTAATTCAATCAAGCCCATGAT; TCCAAGCTCAAGTAACAAGTTTTATATTCTTGAAGCAAACAAGAAAAGGCAATCAATGCT; TAAGTGGTATATTTTAGTATATATTACATTTCTGGACAATCTCTCATTGCATTCAATTTT; TAGAACTTGTCTTGGGTCTAATAAAACAAAAGAATTAGAGAAGAAAGCCCATGCCACTTT; GAGGAATGTAACATAATTGTTTTGTCTGGGTACAAAGGTACGTTACAACACTGGGGGTCT; TTAGTCAAGTTCCCATACTAATTGACTCACCAAAGCATACTTCCTTCAGCTACAACTGTA; ATTGGAACACACAGATTTTACCTGAAGAAAATCCTGTACTTCCAGTTTTAAAAAGCAGAC; AATTCTATGAAGAAGCCACCATTTAAATATCTAACTATACACATTCAAGTTTTTACAACT; TCCTGCCTTATGAGGAATTTTGATTTCAGAAGTATCATTCACAGACTCAACATCATATAT; TTATACATATTTCAAACAGTTTTAGTTTAAGAAACTGTAAAGGGCCCAGAAAAATCCTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_175876
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_175876.3, NP_787072.2
• RefSeq Size: 5099 bp
• RefSeq ORF: 2178 bp
• Locus ID: 149371
• UniProt ID: Q8IYI6
• Cytogenetics: 1q42.2
• Summary: This gene encodes a component of the exocyst complex, an evolutionarily conserved multi-protein complex that plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. This protein is a target of activated Ral subfamily of GTPases and thereby regulates exocytosis by tethering vesicles to the plasma membrane. Mutations in this gene may be related to Joubert syndrome. [provided by RefSeq, Sep 2016]