PPP2R5D (NM_180976) Human Untagged Clone

CAT#: SC127993

PPP2R5D (untagged)-Human protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5D
• Synonyms: B56D; B56delta; MRD35
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_180976, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCTATAAACTGAAAAAGGAGAAGGAGCCCCCCAAGGTTGCCAAATGCACAGCCAAGCCTAGCAGCT
  CGGGCAAGGATGGTGGAGGCGAGAACACTGAGGAGGCCCAGCCGCAGCCCCAGCCCCAGCCCCAGCCCCA
  AGCCCAGTCTCAGCCACCGTCATCCAACAAGCGTCCCAGCAATAGCACGCCGCCCCCCACGCAGCTCAGC
  AAAATCAAGTACTCAGGGGGGCCCCAGATTGTCAAGAAGGAGCTGTTTATCCAGAAGCTACGCCAGTGCT
  GTGTCCTCTTTGACTTCGTGTCAGACCCACTCAGTGACCTCAAATTCAAGGAGGTGAAGCGGGCAGGACT
  CAACGAGATGGTGGAGTACATCACCCATAGCCGTGATGTTGTCACTGAGGCCATTTACCCTGAGGCTGTC
  ACCATGTTTTCAGTGAACCTCTTCCGGACGCTGCCACCTTCATCGAATCCCACAGGGGCTGAGTTTGACC
  CAGAGGAAGATGAGCCCACCCTGGAAGCTGCTTGGCCACATCTCCAGCTCGTGTATGAGTTCTTCTTACG
  TTTCCTTGAGTCTCCTGATTTCCAGCCAAACATAGCCAAGAAGTACATCGACCAGAAGTTTGTACTTGCT
  CTCCTAGACCTATTTGACAGTGAGGATCCTCGAGAGCGGGACTTCCTCAAGACCATTTTGCATCGCATCT
  ATGGCAAGTTTTTGGGGCTCCGGGCTTATATCCGTAGGCAGATCAACCACATCTTCTACAGGTTCATCTA
  CGAGACGGAGCATCACAACGGGATTGCTGAGCTCCTGGAGATCCTGGGCAGCATCATCAATGGCTTTGCC
  CTGCCCCTTAAAGAAGAGCACAAGATGTTCCTCATCCGTGTCCTACTTCCCCTTCACAAGGTCAAGTCCC
  TGAGTGTCTACCACCCTCAGCTGGCATACTGTGTGGTACAATTCCTGGAGAAGGAGAGCAGTCTGACTGA
  GCCGGTAATTGTGGGACTTCTCAAGTTTTGGCCCAAGACCCACAGCCCCAAGGAGGTGATGTTCTTGAAT
  GAGCTGGAGGAGATTCTGGACGTCATTGAACCTTCTGAGTTCAGCAAAGTGATGGAACCCCTCTTCCGCC
  AGCTGGCCAAGTGTGTCTCTAGCCCCCATTTCCAGGTGGCAGAGCGTGCTCTCTATTACTGGAACAATGA
  GTACATCATGAGCCTGATAAGTGACAATGCTGCCCGAGTCCTCCCCATCATGTTCCCTGCACTCTACAGG
  AACTCCAAGAGCCACTGGAACAAGACAATCCATGGACTGATCTATAATGCCCTGAAGTTGTTTATGGAAA
  TGAATCAGAAGCTGTTTGATGACTGCACACAACAATACAAGGCAGAGAAGCAGAAGGGCCGGTTCCGAAT
  GAAGGAAAGGGAAGAGATGTGGCAAAAAATCGAGGAGCTGGCCCGGCTTAATCCCCAGTATCCCATGTTC
  CGAGCCCCTCCACCACTGCCCCCTGTGTACTCGATGGAGACAGAGACCCCCACAGCTGAGGACATCCAGC
  TTCTGAAGAGGACTGTGGAGACTGAGGCTGTTCAGATGCTAAAAGACATCAAGAAGGAGAAAGTGCTGCT
  GCGGAGGAAGTCGGAGCTGCCCCAGGACGTGTACACCATCAAGGCACTGGAGGCGCACAAGCGGGCGGAA
  GAGTTCCTAACTGCCAGCCAGGAGGCTCTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_180976 unedited
  TCCCGCCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA
  GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT
  TCGGCACGAGGTCGACCCGGGCGCAGCGCGCAGGCGGTGGCGAAGAGACGCCGAGCGGGC
  CGAGTGCGGCCGAGCAAAGCCGGAGCCGGAGCGGGGCCGCAGGAGACGGGCCGGGTCCGG
  ACGGGCCGAGATGCCCTATAAACTGAAAAAGGAGAAGGAGCCCCCCAAGGTTGCCAAATG
  CACAGCCAAGCCTAGCAGCTCGGGCAAGGATGGTGGAGGCGAGAACACTGAGGAGGCCCA
  GCCGCAGCCCCAGCCCCAGCCCCAGCCCCAAGCCCAGTCTCAGCCACCGTCATCCAACAA
  GCGTCCCAGCAATAGCACGCCGCCCCCCACGCAGCTCAGCAAAATCAAGTACTCAGGGGG
  GCCCCAGATTGTCAAGAAGGAGCTGTTTATCCAGAAGCTACGCCAGTGCTGTGTCCTCTT
  TGACTTCGTGTCAGACCCACTCAGTGACCTCAAATTCAAGGAGGTGAAGCGGGCAGGACT
  CAACGAGATGGTGGAGTACATCACCCATAGCCGTGATGTTGTCACTGAGGCCATTTACCC
  TGAGGCTGTCACCATGTTTTCAGTGAACCTCTTCCGGACGCTGCCACCTTCATCGAATCC
  CACAGGGGCTGAGTTTGACCCAGAGGAAGATGAGCCCACCCTGGNAGCTGCTTGCCACAT
  CTNCAGCTCGTGTATGAGTTCTTCTTACGTTTCCTTGAGTCTNCTGATTTTCAGCCAACA
  TAGCCCAGAAGTACATCGACCAGAAGTTTGTACTTGCTCTTCTAGACCTATTTGACAGTG
  AGGNATTCTCGAGAGCGGNACTTTCTTCAGACCATTTTGCATCGCATCTTTGGNCAGTTT
  TTTGGGCTTCGGNCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_180976 unedited
  NCCCATCCGATGGGTCCATTGGCGATGGTCAACTTCCCAGGNCCAGNGNGAGCACTGGGG
  NAGTGGGTCACAGGGCATGCCACCCGGGCATCTGTTCAGGAAAAGCTATGACCGCGGCCG
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTCAGAGACCCACTTTATTCAGTTCTG
  TACATATGGGGACATCGGTCCAAGCCCAACCCACCTTAGCATGTATCACTCTGTGGAGAA
  TAAAGCACCCTATGTACACAGCCAAAAGCCGCACTGCCTGCGCCCCTGGAACCTGGGTCC
  GGCCTTCCTCAGCCCCTGGCCCCTCCAAGGTACCTATAAGGGACAAGAGAAGGCCTGCCC
  AGCATAGCACAAACAAGGAAATAAATAGGTGTTGGCAGGAGCCATGCTTGGGGCACCGCT
  CCCTGCTTCCTTCTTTCTAGTTTTGGGAACCAACAGCACAGAAAAGCAGTCAGCCCCAGG
  GTGGTCCCTTCCATGTCTGTGACCCCAGGGCTGCTGCTCACCAAAGACAGGGCACGGGGT
  AGGGTGTTTATTCATCCCTCCCCGGCCCCACTTGGTCCATCTGGGCCCTATATTCATCAG
  TTATTGTCCACAGCCTATACTTTTCTCCTTTTGACCTCCCTTGGTCAGGCATGGGGTAAG
  AAGAAATAATACAACCCTATCTGCCAGCACACGGCTGGGACTGGGGGGCCTATTTGTGCT
  GCAGGGGGGTTGGCTTCCCTTCAGCTACTCTGGGACCAATGGGGCTTGACATAACTTTGG
  TGAGAATAATCTCCTGCTGTTCCCTACATGCCTCTGCAGGGGAAAAGAGAGGAAGGGNAG
  ATACCTGGCGTTCAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_180976
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_180976.1, NP_851307.1
• RefSeq Size: 2871 bp
• RefSeq ORF: 1713 bp
• Locus ID: 5528
• UniProt ID: Q14738
• Cytogenetics: 6p21.1
• Protein Families: Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• Gene Summary: The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (2), that is missing an internal segment compared to isoform 1.