Leptin Receptor (LEPR) (NM_002303) Human Untagged Clone

CAT#: SC127870

LEPR (untagged)-Human leptin receptor (LEPR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Leptin Receptor
• Synonyms: CD295; LEP-R; LEPRD; OB-R; OBR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127870 sequence for NM_002303 edited (data generated by NextGen Sequencing)
  ATGATTTGTCAAAAATTCTGTGTGGTTTTGTTACATTGGGAATTTATTTATGTGATAACT
  GCGTTTAACTTGTCATATCCAATTACTCCTTGGAGATTTAAGTTGTCTTGCATGCCACCA
  AATTCAACCTATGACTACTTCCTTTTGCCTGCTGGACTCTCAAAGAATACTTCAAATTCG
  AATGGACATTATGAGACAGCTGTTGAACCTAAGTTTAATTCAAGTGGTACTCACTTTTCT
  AACTTATCCAAAACAACTTTCCACTGTTGCTTTCGGAGTGAGCAAGATAGAAACTGCTCC
  TTATGTGCAGACAACATTGAAGGAAGGACATTTGTTTCAACAGTAAATTCTTTAGTTTTT
  CAACAAATAGATGCAAACTGGAACATACAGTGCTGGCTAAAAGGAGACTTAAAATTATTC
  ATCTGTTATGTGGAGTCATTATTTAAGAATCTATTCAGGAATTATAACTATAAGGTCCAT
  CTTTTATATGTTCTGCCTGAAGTGTTAGAAGATTCACCTCTGGTTCCCCAAAAAGGCAGT
  TTTCAGATGGTTCACTGCAATTGCAGTGTTCATGAATGTTGTGAATGTCTTGTGCCTGTG
  CCAACAGCCAAACTCAACGACACTCTCCTTATGTGTTTGAAAATCACATCTGGTGGAGTA
  ATTTTCCGGTCACCTCTAATGTCAGTTCAGCCCATAAATATGGTGAAGCCTGATCCACCA
  TTAGGTTTGCATATGGAAATCACAGATGATGGTAATTTAAAGATTTCTTGGTCCAGCCCA
  CCATTGGTACCATTTCCACTTCAATATCAAGTGAAATATTCAGAGAATTCTACAACAGTT
  ATCAGAGAAGCTGACAAGATTGTCTCAGCTACATCCCTGCTAGTAGACAGTATACTTCCT
  GGGTCTTCGTATGAGGTTCAGGTGAGGGGCAAGAGACTGGATGGCCCAGGAATCTGGAGT
  GACTGGAGTACTCCTCGTGTCTTTACCACACAAGATGTCATATACTTTCCACCTAAAATT
  CTGACAAGTGTTGGGTCTAATGTTTCTTTTCACTGCATCTATAAGAAGGAAAACAAGATT
  GTTCCCTCAAAAGAGATTGTTTGGTGGATGAATTTAGCTGAGAAAATTCCTCAAAGCCAG
  TATGATGTTGTGAGTGATCATGTTAGCAAAGTTACTTTTTTCAATCTGAATGAAACCAAA
  CCTCGAGGAAAGTTTACCTATGATGCAGTGTACTGCTGCAATGAACATGAATGCCATCAT
  CGCTATGCTGAATTATATGTGATTGATGTCAATATCAATATCTCATGTGAAACTGATGGG
  TACTTAACTAAAATGACTTGCAGATGGTCAACCAGTACAATCCAGTCACTTGCGGAAAGC
  ACTTTGCAATTGAGGTATCATAGGAGCAGCCTTTACTGTTCTGATATTCCATCTATTCAT
  CCCATATCTGAGCCCAAAGATTGCTATTTGCAGAGTGATGGTTTTTATGAATGCATTTTC
  CAGCCAATCTTCCTATTATCTGGCTACACAATGTGGATTAGGATCAATCACTCTCTAGGT
  TCACTTGACTCTCCACCAACATGTGTCCTTCCTGATTCTGTGGTGAAGCCACTGCCTCCA
  TCCAGTGTGAAAGCAGAAATTACTATAAACATTGGATTATTGAAAATATCTTGGGAAAAG
  CCAGTCTTTCCAGAGAATAACCTTCAATTCCAGATTCGCTATGGTTTAAGTGGAAAAGAA
  GTACAATGGAAGATGTATGAGGTTTATGATGCAAAATCAAAATCTGTCAGTCTCCCAGTT
  CCAGACTTGTGTGCAGTCTATGCTGTTCAGGTGCGCTGTAAGAGGCTAGATGGACTGGGA
  TATTGGAGTAATTGGAGCAATCCAGCCTACACAGTTGTCATGGATATAAAAGTTCCTATG
  AGAGGACCTGAATTTTGGAGAATAATTAATGGAGATACTATGAAAAAGGAGAAAAATGTC
  ACTTTACTTTGGAAGCCCCTGATGAAAAATGACTCATTGTGCAGTGTTCAGAGATATGTG
  ATAAACCATCATACTTCCTGCAATGGAACATGGTCAGAAGATGTGGGAAATCACACGAAA
  TTCACTTTCCTGTGGACAGAGCAAGCACATACTGTTACGGTTCTGGCCATCAATTCAATT
  GGTGCTTCTGTTGCAAATTTTAATTTAACCTTTTCATGGCCTATGAGCAAAGTAAATATC
  GTGCAGTCACTCAGTGCTTATCCTTTAAACAGCAGTTGTGTGATTGTTTCCTGGATACTA
  TCACCCAGTGATTACAAGCTAATGTATTTTATTATTGAGTGGAAAAATCTTAATGAAGAT
  GGTGAAATAAAATGGCTTAGAATCTCTTCATCTGTTAAGAAGTATTATATCCATGATCAT
  TTTATCCCCATTGAGAAGTACCAGTTCAGTCTTTACCCAATATTTATGGAAGGAGTGGGA
  AAACCAAAGATAATTAATAGTTTCACTCAAGATGATATTGAAAAACACCAGAGTGATGCA
  GGTTTATATGTAATTGTGCCAGTAATTATTTCCTCTTCCATCTTATTGCTTGGAACATTA
  TTAATATCACACCAAAGAATGAAAAAGCTATTTTGGGAAGATGTTCCGAACCCCAAGAAT
  TGTTCCTGGGCACAAGGACTTAATTTTCAGAAGCCAGAAACGTTTGAGCATCTTTTTATC
  AAGCATACAGCATCAGTGACATGTGGTCCTCTTCTTTTGGAGCCTGAAACAATTTCAGAA
  GATATCAGTGTTGATACATCATGGAAAAATAAAGATGAGATGATGCCAACAACTGTGGTC
  TCTCTACTTTCAACAACAGATCTTGAAAAGGGTTCTGTTTGTATTAGTGACCAGTTCAAC
  AGTGTTAACTTCTCTGAGGCTGAGGGTACTGAGGTAACCTATGAGGACGAAAGCCAGAGA
  CAACCCTTTGTTAAATACGCCACGCTGATCAGCAACTCTAAACCAAGTGAAACTGGTGAA
  GAACAAGGGCTTATAAATAGTTCAGTCACCAAGTGCTTCTCTAGCAAAAATTCTCCATTG
  AAGGATTCTTTCTCTAATAGCTCATGGGAGATAGAGGCCCAGGCATTTTTTATATTATCA
  GATCAGCATCCCAACATAATTTCACCACACCTCACATTCTCAGAAGGATTGGATGAACTT
  TTGAAATTGGAGGGAAATTTCCCTGAAGAAAATAATGATAAAAAGTCTATCTATTATTTA
  GGGGTCACCTCAATCAAAAAGAGAGAGAGTGGTGTGCTTTTGACTGACAAGTCAAGGGTA
  TCGTGCCCATTCCCAGCCCCCTGTTTATTCACGGACATCAGAGTTCTCCAGGACAGTTGC
  TCACACTTTGTAGAAAATAATATCAACTTAGGAACTTCTAGTAAGAAGACTTTTGCATCT
  TACATGCCTCAATTCCAAACTTGTTCTACTCAGACTCATAAGATCATGGAAAACAAGATG
  TGTGACCTAACTGTGTAA
  
  Clone variation with respect to NM_002303.5
  326 a=>g;668 a=>g;3057 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002303 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGGAGGGCGCGGGGCG
  CACGCGGGCGGCCACCCGACTCTTCCCTCCCTTCTCCCCACCCCAGCCTGCTCCTGCTCA
  GCCCCACCTCCCCACGCACTCGGCTGCCCTCCTCCTCTCCTGAGAGTTGCCCCGCACCTT
  GGGCGAGGAGTTCGGAGCGGCCCCATCGCAGAGCCCACGGCCAGCCGAGCGCGCGCGACG
  CAAGTGCCCGAGCCCCGGCGCCGCCGCCATCTCTGCCTTCGGTCGAGTTGGACCCCCGGA
  TCAAGGTGTACTTCTCTGAAGTAAGATGATTTGTCAAAAATTCTGTGTGGTTTTGTTACA
  TTGGGAATTTATTTATGTGATAACTGCGTTTAACTTGTCATATCCAATTACTCCTTGGAG
  ATTTAAGTTGTCTTGCATGCCACCAAATTCAACCTATGACTACTTCCTTTTGCCTGCTGG
  ACTCTCANAGAATACTTCANATTCGAATGGACATTATGAGACAGCTGTTGAACCTAAGTT
  AATTCAAGTGGTACTCACTTTTCTTACTTATCCAAAAACACTTTCCACTGTTGCTTTTCG
  AGTGAGCAAGATAGAAACTGCTCCTTATGTGCAGACAACATGGANGGAAGGCATTTGTTT
  AACAGTTAATTCTTAAGTTTTCAACAATAGATGCAACTGGAACATCCTTGTTGCTAAAAG
  AAAATAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002303 unedited
  TGGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC
  TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTGGACAATTGGCAATCTAACTTTATCAGAAAGTGACCACTTTAACAT
  CTTTCAGATAGAAGAATTAAAACTGGCATTTTGTTTTCAAAAGTGCTTGTGTTGTCATCA
  AAATACTGATTGTGGGCATCACCATTCTTGCCGGGTATTTCATTCTATGGGACATATCTT
  AGATTTGGTACATAACAATTGGCTCATTAGTCTTCACAAGTATAACCCCTTATGCCAGGG
  CTGAATTCTGTTTATCTTAAACATTTAGGAAAGTCCTACTTCAAATCCTACAGTCTGGTA
  GCGCCAAATATAAATTCCTTATATACTCTCTAAACACACAAGGACATGTGTTAAGAATGT
  GTGTGTGTGTGTGTTGGTTCAAAACAAAAACTTGAGTAAACAACACGCCTCTGAAAAACA
  ATAATCTCCAAAAGATGTGTCTGTTGGCTTATTCTGCTGAACAGGGACNTCCCCTAAGCT
  TGGATAAATAAAAACTGGCTCATTGAGGCTATAAGCTCTAATTTTGCTTGTCTAGGAGAC
  AACGCACATCTTGGACATTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002303
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002303.2, NP_002294.2
• RefSeq Size: 4102 bp
• RefSeq ORF: 3498 bp
• Locus ID: 3953
• UniProt ID: P48357
• Cytogenetics: 1p31.3
• Domains: FN3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1).