MLH3 (NM_014381) Human Untagged Clone

CAT#: SC127828

MLH3 (untagged)-Human mutL homolog 3 (E. coli) (MLH3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MLH3
• Synonyms: HNPCC7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127828 sequence for NM_014381 edited (data generated by NextGen Sequencing)
  ATGATCAAGTGCTTGTCAGTTGAAGTACAAGCCAAATTGCGTTCTGGTTTGGCCATAAGC
  TCCTTGGGCCAATGTGTTGAGGAACTTGCCCTCAACAGTATTGATGCTGAAGCAAAATGT
  GTGGCTGTCAGGGTGAATATGGAAACCTTCCAAGTTCAAGTGATAGACAATGGATTTGGG
  ATGGGGAGTGATGATGTAGAGAAAGTGGGAAATCGTTATTTCACCAGTAAATGCCACTCG
  GTACAGGACTTGGAGAATCCAAGGTTTTATGGTTTCCGAGGAGAGGCCTTGGCAAATATT
  GCTGACATGGCCAGTGCTGTGGAAATTTCGTCCAAGAAAAACAGGACAATGAAAACTTTT
  GTGAAACTGTTTCAGAGTGGAAAAGCCCTGAAAGCTTGTGAAGCTGATGTGACTAGAGCA
  AGCGCTGGGACTACTGTAACAGTGTATAACCTATTTTACCAGCTTCCTGTAAGGAGGAAA
  TGCATGGACCCTAGACTGGAGTTTGAGAAGGTTAGGCAGAGAATAGAAGCTCTCTCACTC
  ATGCACCCTTCCATTTCTTTCTCTTTGAGAAATGATGTTTCTGGTTCCATGGTTCTTCAG
  CTCCCTAAAACCAAAGACGTATGTTCCCGATTTTGTCAAATTTATGGATTGGGAAAGTCC
  CAAAAGCTAAGAGAAATAAGTTTTAAATATAAAGAGTTTGAGCTTAGTGGCTATATCAGC
  TCTGAAGCACATTACAACAAGAATATGCAGTTTTTGTTTGTGAACAAAAGACTAGTTTTA
  AGGACAAAGCTACATAAACTCATTGACTTTTTATTAAGGAAAGAAAGTATTATATGCAAG
  CCAAAGAATGGTCCCACCAGTAGGCAAATGAATTCAAGTCTTCGGCACCGGTCTACCCCA
  GAACTCTATGGCATATATGTAATTAATGTGCAGTGCCAATTCTGTGAGTATGATGTGTGC
  ATGGAGCCAGCCAAAACTCTGATTGAATTTCAGAACTGGGACACTCTCTTGTTTTGCATT
  CAGGAAGGAGTGAAAATGTTTTTAAAGCAAGAAAAATTATTTGTGGAATTATCAGGTGAG
  GATATTAAGGAATTTAGTGAAGATAATGGTTTTAGTTTATTTGATGCTACTCTTCAGAAG
  CGTGTGACTTCCGATGAGAGGAGCAATTTCCAGGAAGCATGTAATAATATTTTAGATTCC
  TATGAGATGTTTAATTTGCAGTCAAAAGCTGTGAAAAGAAAAACTACTGCAGAAAACGTA
  AACACACAGAGTTCTAGGGATTCAGAAGCTACCAGAAAAAATACAAATGATGCATTTTTG
  TACATTTATGAATCAGGTGGTCCAGGCCATAGCAAAATGACAGAGCCATCTTTACAAAAC
  AAAGACAGCTCTTGCTCAGAATCAAAGATGTTAGAACAAGAGACAATTGTAGCATCAGAA
  GCTGGAGAAAATGAGAAACATAAAAAATCTTTCCTGGAACATAGCTCTTTAGAAAATCCG
  TGTGGAACCAGTTTAGAAATGTTTTTAAGCCCTTTTCAGACACCATGTCACTTTGAGGAG
  AGTGGGCAGGATCTAGAAATATGGAAAGAAAGTACTACTGTTAATGGCATGGCTGCCAAC
  ATCTTGAAAAATAATAGAATTCAGAATCAACCAAAGAGATTTAAAGATGCTACTGAAGTG
  GGATGCTAGCCTCTGCCTTTTGCAACAACATTATGGGGAGTACATAGTGCTCAGACAGAG
  AAAGAGAAAAAAAAAGAATCTAGCAATTGTGGAAGAAGAAATGTTTTTAGTTATGGGCGA
  GTTAAATTATGTTCCACTGGCTTTATAACTCATGTAGTACAAAATGAAAAAACTAAATCA
  ACTGAAACAGAACATTCATTTAAAAATTATGTTAGACCTGGTCCCACACGTGCCCAAGAA
  ACATTTGGAAATAGAACACGTCATTCAGTTGAAACTCCAGACATCAAAGATTTAGCCAGC
  ACTTTAAGTAAAGAATCTGGTCAATTGCCCAACAAAAAAAATTGCAGAACGAATATAAGT
  TATGGGCTAGAGAATGAACCTACAGCAACTTATACAATGTTTTCTGCTTTTCAGGAAGGT
  AGCAAAAAATCACAAACAGATTGCATATTATCTGATACATCCCCCTCTTTCCCCTGGTAT
  AGACACGTTTCCAATGATAGTAGGAAAACAGATAAATTAATTGGTTTCTCCAAACCAATC
  GTCCGTAAGAAGCTAAGCTTGAGTTCACAGCTAGGATCTTTAGAGAAGTTTAAGAGGCAA
  TATGGGAAGGTTGAAAATCCTCTGGATACAGAAGTAGAGGAAAGTAATGGAGTCACTACC
  AATCTCAGTCTTCAAGTTGAACCTGACATTCTGCTGAAGGACAAGAACCGCTTAGAGAAC
  TCTGATGTTTGTAAAATCACTACTATGGAGCATAGTGATTCAGATAGTAGTTGTCAACCA
  GCAAGCCACATCCTTGACTCAGAGAAGTTTCCATTCTCCAAGGATGAAGATTGTTTAGAA
  CAACAGATGCCTAGTTTGAGAGAAAGTCCTATGACCCTGAAGGAGTTATCTCTCTTTAAT
  AGAAAACCTTTGGACCTTGAGAAGTCATCTGAATCACTAGCCTCTAAATTATCCAGACTG
  AAGGGTTCCGAAAGAGAAACTCAAACAATGGGGATGATGAGTCGTTTTAATGAACTTCCA
  AATTCAGATTCCAGTAGGAAAGACAGCAAGTTGTGCAGTGTGTTAACACAAGATTTTTGT
  ATGTTATTTAACAACAAGCATGAAAAAACAGAGAATGGTGTCATCCCAACATCAGATTCT
  GCCACACAGGATAATTCCTTTAATAAAAATAGTAAAACACATTCTAACAGCAATACAACA
  GAGAACTGTGTGATATCAGAAACTCCTTTGGTATTGCCCTATAATAATTCTAAAGTTACC
  GGTAAAGATTCAGATGTTCTTATCAGAGCCTCAGAACAACAGATAGGAAGTCTTGACTCT
  CCCAGTGGAATGTTAATGAATCCGGTAGAAGATGCCACAGGTGACCAAAATGGAATTTGT
  TTTCAGAGTGAGGAATCTAAAGCAAGAGCTTGTTCTGAAACTGAAGAGTCAAACACGTGT
  TGTTCAGATTGGCAGCGGCATTTCGATGTAGCCCTGGGAAGAATGGTTTATGTCAACAAA
  ATGACTGGACTCAGCACATTCATTGCCCCAACTGAGGACATTCAGGCTGCTTGTACTAAA
  GACCTGACAACTGTGGCTGTGGATGTTGTACTTGAGAATGGGTCTCAGTACAGGTGTCAA
  CCTTTTAGAAGCGACCTTGTTCTTCCTTTCCTTCCGAGAGCTCGAGCAGAGAGGACTGTG
  ATGAGACAGGATAACAGAGATACTGTGGATGATACTGTTAGTAGCGAATCGCTTCAGTCT
  TTGTTCTCAGAATGGGACAATCCAGTATTTGCCCGTTATCCAGAGGTTGCTGTTGATGTA
  AGCAGTGGCCAGGCTGAGAGCTTAGCAGTTAAAATTCACAACATCTTGTATCCCTATCGT
  TTCACCAAAGGAATGATTCATTCAATGCAGGTTCTCCAGCAAGTAGATAACAAGTTTATT
  GCCTGTTTGATGAGCACTAAGACTGAAGAGAATGGCGAGGCAGATTCCTACGAGAAGCAA
  CAGGCACAAGGCTCTGGTCGGAAAAAATTACTGTCTTCTACTCTAATTCCTCCGCTAGAG
  ATAACAGTGACAGAGGAACAAAGGAGACTCTTATGGTGTTACCACAAAAATCTGGAAGAT
  CTGGGCCTTGAATTTGTATTTCCAGACACTAGTGATTCTCTGGTCCTTGTGGGAAAAGTA
  CCACTATGTTTTGTGGAAAGAGAAGCCAATGAACTTCGGAGAGGAAGATCTACTGTGACC
  AAGAGTATTGTGGAGGAATTTATCCGAGAACAACTGGAGCTACTCCAGACCACCGGAGGC
  ATCCAAGGGACATTGCCACTGACTGTCCAGAAGGTGTTGGCATCCCAAGCCTGCCATGGG
  GCCATTAAGTTTAATGATGGCCTGAGCTTACAGGAAAGTTGCCGCCTTATTGAAGCTCTG
  TCCTCATGCCAGCTGCCATTCCAGTGTGCTCACGGGAGACCTTCTATGCTGCCGTTAGCT
  GACATAGACCACTTGGAACAGGAAAAACAGATTAAACCCAACCTCACTAAACTTCGCAAA
  ATGGCCCAGGCCTGGCGTCTCTTTGGAAAAGCAGAGTGTGATACAAGGCAGAGCCTGCAG
  CAGTCCATGCCTCCCTGTGAGCCACCATGA
  
  Clone variation with respect to NM_014381.2
  1687 c=>t;2476 a=>g;4263 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014381 unedited
  NGGTTCACAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCGCACGAGC
  CTCAAGACCAGGTGCGCGCGTCGGCGTCCGAGGCGGTTGGTGTCGGAGAATTTGTTAAGC
  GGGACTCCAGGCAATTATTTCCAGTCAGAGAAGGAAACCAGTGCCTGGCATTCTCACCAT
  CTTTCTACCTACCATGATCAAGTGCTTGTCAGTTGAAGTACAAGCCAAATTGCGTTCTGG
  TTTGGCCATAAGCTCCTTGGGCCAATGTGTTGAGGAACTTGCCCTCAACAGTATTGATGC
  TGAAGCAAAATGTGTGGCTGTCAGGGTGAATATGGAAACCTTCCAAGTTCAAGTGATAGA
  CAATGGATTTGGGATGGGGAGTGATGATGTAGAGAAAGTGGGAAATCGTTATTTCACCAG
  TAAATGCCACTCGGTACAGGACTTGGAGAATCCAAGGTTTTATGGTTTCCGAGGAGAGGC
  CTTGGCAAATATTGCTGACATGGCCAGTGCTGTGGAAATTTCGTCCAAGAAAAACAGGAC
  AATGAAAACTTTTGTGAAACTGTTTCAGAGTGGAAAAGCCCTGAAAGCTTGTGAAGCTGA
  TGTGACTAGAGCAAGCGCTGGGACTACTGTAACAGTGTATAACCTATTTTACCAGCTNTC
  CTGTAGGAGGAAATGCATGGACCCTAGACTGGAGTTTGAGAAGGTTAGGCAGAGAATAGA
  AGCTCTCTCACTCATGCACCCTTCCATTTCTTTCTCTTTGAGAAATGATGTTTCTGGTTC
  CATGGTTCCTCAGCTCCCCTAAACCAAAGACGTATGTTCCCGATTTTGTCAATTTATGGA
  TGGNGAAAGTCCCAAAGCTAAGAGAATAAGTTTAAATTTAAAGAGTTGAGCTTATGGCTT
  ATCAGCTCTGAGCCCTTACACAAGAATTGCAGTTTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014381 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCAAACTGTCATTAAAT
  TTTATCATTAAATAAGTAGCAAAAGGTAGATACTATATAGCAACCTTCTGTGCCCATGGA
  TGCAAATGAATGAATTGTCTTTAGGCTTGAATTTCATCTGGCTACTCAACTAGGGGAATC
  ATCTGCTCAAGAAAGACTGATACAGAGAGCCCTGCTGTCTAAGCTGCTCAGGGACACTGG
  GCTGATTCAGTCAGGGCCGTGCTGGTACCTGCTGCTGCTGCTCTCTGCTCAGAGGCATAC
  AGTGAACATCCCTTTGTTCCTTTTAGACCAGTGATTCTGTCTTCATGGTGGCTCACAGGG
  AGGCATGGACTGTTGCAGGCCTCTGCCTTGTATCACACTCTGCTTTCTCCAAGCACACGC
  CAGTCCTGGGCCCCTTCGCTCAATTCCCTGCGGCCCCCCTTATCCCCCTTTTCCTGTCCC
  CTCCCCCCCCCCTCTCCCTCCCCTCTCTCCCTCCTCCCCTCTCCCCCTTTTCCCCTTTCC
  CCTCCCCCCATTCCCTCCATTCCTCCTCCTCTCTCCTCTCCCTTTTTCCCCTCCCCTCCC
  CCTTCCTTTCTCTTCCCCCTCCCTTCCCCCTTTTTTTTCTCCCCCCCCTCCCCCCCCCTT
  TCCCCCCTTCCCCCCATCTCTCTCCTCCCCTACCTCATTCTGCCTCCCCCCACCCCTCTT
  TCATTTCCTTCCCACCCCCCATCTCCCTCTTCTCCCCCCTCTCCCCCCTTTCCCCTTTTC
  CTCCCTCCCTTTTCCCTTCTTTCCCTCCTCCCCCCTCCCCTTTCTCCCCCCCCCCCTTTC
  CCCTTCCTCCTCTTCCTCCCCTCCCCCCCTCTATCCCCCCTCCTCTCCCCTTTTTTTTCC
  CCATCCCCCCTCTTTCTTTCCTTTTACTCCCCCCCCCCCCCTCTTCCTTTCACCTCCCCC
  CTTCTTACTCCTCATTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014381
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014381.1, NP_055196.1
• RefSeq Size: 4895 bp
• RefSeq ORF: 4290 bp
• Locus ID: 27030
• Cytogenetics: 14q24.3
• Domains: HATPase_c
• Protein Families: Druggable Genome
• Protein Pathways: Mismatch repair
• Gene Summary: This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2), also known as delta7, lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.