RPL29 (AF116627) Human Untagged Clone

CAT#: SC127782

(untagged)-Human PRO1181 mRNA, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL29
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AF116627, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCCGGAAGATCACCGAGGTGCTAGTACGAAAGGTCCCTGACAACCAACAGTTCCTAGACCTCCGTG
  TGGCCGTCCTGGGGAATGTGGACTCTGGGAAGTCAACTCTGCTTGGAGTCCTGACCCAGGGAGAGCTGGA
  CAATGGGCGGGGCCGGGCTCGGCTCAACCTTTTCCGCCACCTGCATGAGATTCAGTCTGGCCGAACCTCC
  AGCATCAGCTTCGAGATCCTGGGCTTTAACAGCAAGGGAGAGGTGCATGGGATCAATGGGACCCAATGGG
  GCCAGACTCTGAGGATGGGATGGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AF116627 unedited
  GCACGAGGGCGCTTCGGGAGCCGCGGCTTATGGTGCAGACATGGCCAAGTCCAAGAACCA
  CACCACACACAACCAGTCCCGAAAATGGCACAGAAATGGTATCAAGAAACCCCGATCACA
  AAGATACGAATCTCTTAAGGGGGTGGACCCCAAGTTCCTGAGGAACATGCGCTTTGCCAA
  GAAGCACAACAAAAAGGGCCTAAAGAAGATGCAGGCCAACAATGCCAAGGCCATGAGTGC
  ACGTGCCGAGGCTATCAAGGCCCTCGTAAAGCCCAAGGAGGTTAAGCCCAAGATCCCAAA
  GGGTGTCAGCCGCAAGCTCGATCGACTTGCCTACATTGCCCACCCCAAGCTTGGGAAGCG
  TGCTCGTGCCCGTATTGCCAAGGGGCTCAGGCTGTGCCGGCCAAAGGCCAAGGCCAAGGC
  CAAGGCCAAGGATCAAACCAAGGCCCAGGCTGCAGCCCCAGCTTCAGTTCCAGCTCAGGC
  TCCCAAACGTACCCAGGCCCCTACAAAGGCTTCAGAGTAGATATCTCTGCCAACATGAGG
  ACAGAAGGACTGGTGCGACCCCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AF116627 unedited
  CCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTCCCGGCCTAAGGTTTATTTGTACAAATAGCACAGGA
  GGACCCCACCCCCATGCAAATGGTACCCCAGGGGCGGGGGGGGGGGGTCAAACCAATCCT
  TTTGTCTTAATGTTGGAAAAAATATTTACTTTAAACCCTTTGAAGGGCCCTGGGTACTTT
  TGGAACCCTAACCTGAAACTAAACCTGGGGCTGAACCCTGGCCCTTGGTTAAATCCTTGG
  CCTTGGCCTTGGCCTTGGCCTTTGGCCGGAACAACCTGACCCCTTTGGAAAAACGGGCAA
  AAACACCCTTCCAAAGTTTGGGGGGGGCAATGTAGGCAAGTCAATCAACTTTGGGGTTAA
  CACCCTTTGGAATCTTGGGCTAAACCTCCTTGGGCTTAACAAGGGCCTTAATAACTTTGG
  CACGGGCACTCATGGCCTTGGAATTGTGGGCCCCGTATTTTTTTAGGCCCTTTTTGTTGC
  CCTTCTTGGAAAGACCCATTGTCTCTAAAGAACTTGGGGCCCCCCCCCTTAAGAAAATCC
  ATTTTTTGGAACCAGGGTTTTTTGAAACCATTTTTGGGCCATTTTAGGGACTGGTTGGGT
  GCGGGGGGGTCTTTGAACTTGGCCACTTTTCACAATAAAACCCGGGTTCCCAAAACGCCT
  TTTGCCAAATTCGGGGCCCCCTAAATGAATTCCTATAAAAAATTTGAAAGGTCAAAAAAA
  AAAATTTTTTATTATAAACATTCAATGTAAACCCTTTATGCCATTTTGGTAAACGGGGGC
  GGGTATTAAAAACATTGGGAAAACTCCCTTATTTTGGCCCAAAAAAAATCCTCATTGTTA
  AAGTGGTGGAAAACTTGAAACCCGGTGAACAAACCTTTCCCCCCTTTTGGTC
  
• Restriction Sites: NotI-NotI
• ACCN: AF116627
• Insert Size: 750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AF116627.1, AAF71050.1
• RefSeq Size: 1487 bp
• RefSeq ORF: 1487 bp
• Locus ID: 6159
• Cytogenetics: 3p21.2
• Domains: Ribosomal_L29e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]