CXCL14 (NM_004887) Human Untagged Clone

CAT#: SC127695

CXCL14 (untagged)-Human chemokine (C-X-C motif) ligand 14 (CXCL14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CXCL14
• Synonyms: BMAC; BRAK; KEC; KS1; MIP-2g; MIP2G; NJAC; SCYB14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127695 sequence for NM_004887 edited (data generated by NextGen Sequencing)
  ATGTCCCTGCTCCCACGCCGCGCCCCTCCGGTCAGCATGAGGCTCCTGGCGGCCGCGCTG
  CTCCTGCTGCTGCTGGCGCTGTACACCGCGCGTGTGGACGGGTCCAAATGCAAGTGCTCC
  CGGAAGGGACCCAAGATCCGCTACAGCGACGTGAAGAAGCTGGAAATGAAGCCAAAGTAC
  CCGCACTGCGAGGAGAAGATGGTTATCATCACCACCAAGAGCGTGTCCAGGTACCGAGGT
  CAGGAGCACTGCCTGCACCCCAAGCTGCAGAGCACCAAGCGCTTCATCAAGTGGTACAAC
  GCCTGGAACGAGAAGCGCAGGGTCTACGAAGAATAG
  
  Clone variation with respect to NM_004887.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004887 unedited
  GTAATACGACTCACTATAGGGCGGNNNNCGAATTCGGCACGAGNAGAGCGCGGAGACAAG
  CGCAGAGCGCAGCGCACGGNNACAGACAGCCCTGGGCATCCACCGACGGCGCAGCCGGAG
  CCAGCAGAGCCGGTNNNCGCGCCCCGGGCAGAGAAAGCCGAGCAGAGCTGGGTGGCGTCT
  CCGGGCCGNNNCTCCGACGGGCCAGCGCCCTCCCCATGTCCCTGCTCCCACGCCGCGCCC
  CTNNNGTCAGCATGAGGCTCCTGGCGGCCGCGCTGCTCCTGCTGCTGCTGGCGNNNTACA
  CCGCGCGTGTGGACGGGTCCAAATGCAAGTGCTCCCGGAAGGGACCCNNNATCCGCTACA
  GCGACGTGAAGAAGCTGGAAATGAAGCCAAAGTACCCGCACTNNNAGGAGAAGATGGTTA
  TCATCACCACCAAGAGCGTGTCCAGGTACCGAGGTCAGGNNNACTGCCTGCACCCCAAGC
  TGCAGAGCACCAAGCGCTTCATCAAGTGGTACAACGNNNNGAACGAGAAGCGCAGGGTCT
  ACGAAGAATAGGGTGAAAAACCTCAGAAGGGAAAACNNNNAACCAGTTGGGAGACTTGTG
  CANAGGACTTTGCAGATTAAAAAAAAAAAAAAAAAACTAAATNNNNATATTGCGGCNCGC
  GGTCATAGNCTGTTTCCTGAACAGATCCCCGGGTGGCATCCCTTGTGACCCCTNNNNNNA
  AACCTCTCCCTGCGCCCCTGGAGTTGCCACCTCAGTGCCCCACAGCCCTTGCCTAATAAA
  ATAAGTTGCCTTNNNTTTTCTGACTANGNGTCCTTCTATAAATATTGGGTGGAGGGGGGT
  GGTATGGAGCAAGGGCAAGTTGGAAAAACACTNNNNAATCTGCTGGGTTATGGGGACCAA
  GCTGGATGCAGGGCACATTTTGGTTCCTGCATTTCCGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004887 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATCTGCAAAGTCCTT
  TGCACAAGTCTCCCAACTGGTTTGGAGTTTTCCCTTCTGAGGTTTTTCACCCTATTCTTC
  GTAGACCCTGCGCTTCTCGTTCCAGGCGTTGTACCACTTGATGAAGCGCTTGGTGCTCTG
  CAGCTTGGGGTGCAGGCAGTGCTCCTGACCTCGGTACCTGGACACGCTCTTGGTGGTGAT
  GATAACCATCTTCTCCTCGCAGTGCGGGTACTTTGGCTTCATTTCCAGCTTCTTCACGTC
  GCTGTAGCGGATCTTGGGTCCCTTCCGGGAGCACTTGCATTTGGACCCGTCCACACGCGC
  GGTGTACAGCGCCAGCAGCAGCAGGAGCAGCGCGGCCGCCAGGAGCCTCATGCTGACCGG
  AGGGGCGCGGCGTGGGAGCAGGGACATGGGGAGGGCGCTGGCCCGTCGGAGCGGCGGCCC
  GGAGACGCCACCCAGCTCTGCTCGGCTTTCTCTGCCCGGGGCGCGCCTTCCGGCTCTGCT
  GGCTCCGGCTGCGCCGTCGGTGGATGCCCAGGGCTGTCTGTGGCCGTGCGCTGCGCTCTG
  CGCTTGTCTCCGCGCTCTCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTA
  CAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCC
  TACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGAT
  TNTGGTGCCAAAACAAACTCCCATTNGACGTCATGGGGTGGAGACTTGGAAATCCCCGTG
  AGTCAAACCGCTATCCCACGCCATTGGTGTACTGCCAAAACCGCATCACATGGTAATACC
  GATGACTAATACCTAAATGTACTGCCAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004887
• Insert Size: 380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004887.3, NP_004878.2
• RefSeq Size: 1968 bp
• RefSeq ORF: 336 bp
• Locus ID: 9547
• UniProt ID: O95715
• Cytogenetics: 5q31.1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: This antimicrobial gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Sep 2014]