SDF1 (CXCL12) (NM_199168) Human Untagged Clone

CAT#: SC127614

CXCL12 (untagged)-Human chemokine (C-X-C motif) ligand 12 (CXCL12), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SDF1
• Synonyms: IRH; PBSF; SCYB12; SDF1; TLSF; TPAR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127614 sequence for NM_199168 edited (data generated by NextGen Sequencing)
  ATGAACGCCAAGGTCGTGGTCGTGCTGGTCCTCGTGCTGACCGCGCTCTGCCTCAGCGAC
  GGGAAGCCCGTCAGCCTGAGCTACAGATGCCCATGCCGATTCTTCGAAAGCCATGTTGCC
  AGAGCCAACGTCAAGCATCTCAAAATTCTCAACACTCCAAACTGTGCCCTTCAGATTGTA
  GCCCGGCTGAAGAACAACAACAGACAAGTGTGCATTGACCCGAAGCTAAAGTGGATTCAG
  GAGTACCTGGAGAAAGCTTTAAACAAGTAA
  
  Clone variation with respect to NM_199168.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199168 unedited
  NGGGCCCCCCAAACNNCTTCCCCCCCCCGGTTCAGAATTGTATACGACTCACTATAGGCG
  GCCGCGAATTCGCACGAGTCAGCCGCATTGCCCGCTCGGCGTCCGGCCCCCGACCCGCGC
  TCGTCCGCCCGCCCGCCCGCCCGCCCGCGCCATGAACGCCAAGGTCGTGGTCGTGCTGGT
  CCTCGTGCTGACCGCGCTCTGCCTCAGCGACGGGAAGCCCGTCAGCCTGAGCTACAGATG
  CCCATGCCGATTCTTCGAAAGCCATGTTGCCAGAGCCAACGTCAAGCATCTCAAAATTCT
  CAACACTCCAAACTGTGCCCTTCAGATTGTAGCCCGGCTGAAGAACAACAACAGACAAGT
  GTGCATTGACCCGAAGCTAAAGTGGATTCAGGAGTACCTGGAGAAAGCTTTAAACAAGTA
  AGCACAACAGCCAAAAAGGACTTTCCGCTAGACCCACTCGAGGAAAACTAAAACCTTGTG
  AGAGATGAAAGGGCAAAGACGTGGGGGAGGGGGCCTTAACCATGAGGACCANGTGTGTGT
  GTGGGGTGGGCACATTGATCTGGGATCGGGCCTGAGGTTTGCCAGCATTTAGACCCTGCA
  TTTATAGCATACGGTATGATATTGCAGCTTATATTCATCCATGCCCTGTACCTGTGCACG
  TGGNAACTTTNATTACTGGNGGTTTTTCTAAGAAAGAAAATTGTATTATCAACAGCATTT
  TCAAAGCAGTAGTTCCCNCATGATCATCACAATCATCATCATTCTCATTCTCATTTTTTT
  AATCAACGAGTACTTAAAGATCTGAATTTGGGCTTGTTTGGAGCATCTTCTTTGCTCCCC
  TGGGGAGTCTGGCCCAGTCAGGTGTGGGTTAACAGGNAGCTGAAAAAAGGGCCTTTCTTA
  AAAACTGAGGGTCCCGAAAAAGGCCCCTTCCAAAGGAAGCCTTTTGGGCCTACAAACNAC
  TTGGGCTTGCGCCCCCCTTGCCTTACCTTCCTTTTAAACTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199168
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199168.1, NP_954637.1
• RefSeq Size: 1940 bp
• RefSeq ORF: 270 bp
• Locus ID: 6387
• UniProt ID: P48061
• Cytogenetics: 10q11.21
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration
• Gene Summary: This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (1) encodes the shortest protein (isoform alpha).